Molecular mechanisms underlying the glia-mediated osmo-regulation

• Project/Area Number: 23790256
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: KAGE-NADAKAI Eriko 東京女子医科大学, 医学部, 講師 (40453790)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 浸透圧 / グリア細胞 / 線虫 / 生理学 / 神経科学

Research Abstract

We characterized three Caenorhabditis elegansHMIT genes, hmit-1.1, hmit-1.2, and hmit-1.3. hmit-1.1 was expressed in the intestine, and hmit-1.2 was expressed in the glia and the excretory canal, which is an osmotic regulatory organ that is functionally analogous to the kidney. hmit-1.3 was expressed in the intestine and the glia. The expression of hmit-1.1 and hmit-1.2 but not hmit-1.3, was markedly induced under hyperosmotic conditions. Animals with mutant hmit-1.1 and hmit-1.2 were hypersensitive to osmotic stress. The defects of hmit-1.1 and hmit-1.2 mutants were rescued by hmit-1.1 and hmit-1.2 transgenes, respectively, and by modified human HMIT. In human cell lines, HMIT expression was induced in hyperosmotic conditions. These findings indicate that the C. elegans HMIT family has a crucial role in the osmoprotective response.

Research Products

• [Journal Article] Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans (2014)
  • Author(s): Kage-Nakadai E, Imae R, Yoshina S, Mitani S
  • Journal Title: Methods Volume: (Epub ahead of print)
• [Journal Article] The homologous carboxyl-terminal domains of microtubule-associated protein 2 and TAU induce neuronal dysfunction and have differential fates in the evolution of neurofibrillary tangles (2014)
  • Author(s): Xie C, Miyasaka T, Yoshimura S, Hatsuta H, Yoshina S, Kage-Nakadai E, Mitani S, Murayama S, Ihara Y
  • Journal Title: PLoS One Volume: 9
• [Journal Article] Influence of oral supplementation with sesamin on longevity of Caenorhabditis elegans and the host defense (2014)
  • Author(s): Yaguchi Y, Komura T, Kashima N, Tamura M, Kage-Nakadai E, Saeki S, Terao K, Nishikawa Y
  • Journal Title: Eur J Nutr Volume: (Epub ahead of print)
• [Journal Article] Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans. (2014)
  • Author(s): Kage-Nakadai E, Imae R, Yoshina S, Mitani S
  • Journal Title: Methods Volume: Mar 12 Issue: 3 Pages: 91-7
  • DOI: 10.1016/j.ymeth.2014.02.036
  • Peer Reviewed / Open Access
• [Journal Article] The homologous carboxyl-terminal domains of microtubule-associated protein 2 and TAU induce neuronal dysfunction and have differential fates in the evolution of neurofibrillary tangles. (2014)
  • Author(s): Xie, C., T. Miyasaka, S. Yoshimura, H. Hatsuta, S. Yoshina, E. Kage-Nakadai, S. Mitani, Murayama, S., Y. Ihara
  • Journal Title: PLoS One Volume: 9 Issue: 2 Pages: e89796-e89796
  • DOI: 10.1371/journal.pone.0089796
  • Peer Reviewed
• [Journal Article] Mitochondria-type GPAT is required for mitochondrial fusion (2013)
  • Author(s): Ohba Y, Sakuragi T, Kage-Nakadai E, Tomioka NH, Kono N, Imae R, Inoue A, Aoki J, Ishihara N, Inoue T, Mitani S, Arai H
  • Journal Title: EMBO Journal Volume: 32 Pages: 1265-1279
• [Journal Article] Mitochondria-type GPAT is required for mitochondrial fusion (2013)
  • Author(s): Yohsuke Ohba, Takeshi Sakuragi, Eriko Kage-Nakadai, Naoko H Tomioka, Nozomu Kono, Rieko Imae, Asuka Inoue, Junken Aoki, Naotada Ishihara, Takao Inoue, Shohei Mitani and Hiroyuki Arai
  • Journal Title: The EMBO Journal Volume: (in press) Issue: 9 Pages: 1265-1279
  • DOI: 10.1038/emboj.2013.77
  • Peer Reviewed / Open Access
• [Journal Article] large-scale screening for targeted knockouts in the Caenorhabditis elegans genome (2012)
  • Author(s): C. elegans Deletion Mutant Consortium
  • Journal Title: G3 (Bethesda) Volume: 2 Pages: 1415-1425
• [Journal Article] NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane (2012)
  • Author(s): Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y
  • Journal Title: PLoS One Volume: 7
• [Journal Article] Depletion of mboa-7, an enzyme that incorporates polyunsaturated fatty acids into phosphatidylinositol (PI), impairs PI 3-phosphate signaling in Caenorhabditis elegans (2012)
  • Author(s): Lee HC, Kubo T, Kono N, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Inoue T, Arai H
  • Journal Title: Genes to Cells Volume: 17 Pages: 748-757
• [Journal Article] elegans secreted lipid-binding protein NRF-5 mediates PS appearance on phagocytes for cell corpse engulfment (2012)
  • Author(s): Zhang Y, Wang H, Kage-Nakadai E, Mitani S, Wang X. C
  • Journal Title: Current Biology Volume: 22 Pages: 1276-1284
• [Journal Article] Reduced expression of BTBD10, an Akt activator, leads to motor neuron death (2012)
  • Author(s): Nawa M, Kage-Nakadai E, Aiso S, Okamoto K, Mitani S, Matsuoka M
  • Journal Title: Cell Death & Differentiation Volume: 19 Pages: 1398-1407
• [Journal Article] GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans (2012)
  • Author(s): Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
  • Journal Title: Molecular Biology of the Cell Volume: 23 Pages: 982-995
• [Journal Article] Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method (2012)
  • Author(s): Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S, Mitani S
  • Journal Title: BMC Biotechnology Volume: 12 Pages: 1-1
• [Journal Article] C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on phagocytes for cell corpse engulfment. (2012)
  • Author(s): Zhang Y, Wang H, Kage-Nakadai E, Mitani S, Wang X
  • Journal Title: Curr Biol Volume: 22 Issue: 14 Pages: 1276-1284
  • DOI: 10.1016/j.cub.2012.06.004
  • Peer Reviewed
• [Journal Article] Depletion of mboa-7, anenzyme that incorporates polyunsaturated fatty acids into phosphatidylinositol (PI),impairs PI3-phosphate signaling in Caenorhabditis elegans. (2012)
  • Author(s): Lee HC, Kubo T, Kono N, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Inoue T, Arai H
  • Journal Title: Genes Cells Volume: 17(9) Issue: 9 Pages: 748-57
  • DOI: 10.1111/j.1365-2443.2012.01624.x
  • Peer Reviewed
• [Journal Article] NRFL-1, the C. elegans NHERF Orthologue, Interacts with Amino Acid Transporter 6 (AAT-6) for Age-Dependent Maintenance of AAT-6 on the Membrane. (2012)
  • Author(s): Kohei Hagiwara
  • Journal Title: Plos one Volume: 7(8) Issue: 8 Pages: e43050-e43050
  • DOI: 10.1371/journal.pone.0043050
  • Peer Reviewed
• [Journal Article] large-scale screening for targeted knockouts in the Caenorhabditis elegans genome. (2012)
  • Author(s): C. elegans Deletion Mutant Consortium
  • Journal Title: G3 (Bethesda) Volume: 2 Issue: 11 Pages: 1415-25
  • DOI: 10.1534/g3.112.003830
  • Peer Reviewed
• [Journal Article] Reduced Expression of BTBD10, an Akt activator, Drives Impairment of Motor Function (2012)
  • Author(s): Nawa M, et al
  • Journal Title: Cell Death and Differentiation Volume: (in press) Issue: 8 Pages: 1398-1407
  • DOI: 10.1038/cdd.2012.19
  • Peer Reviewed
• [Journal Article] GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans. (2012)
  • Author(s): Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
  • Journal Title: Mol Biol Cell Volume: 23 Pages: 982-995
  • Peer Reviewed
• [Journal Article] Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method. (2012)
  • Author(s): Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S, Mitani S
  • Journal Title: BMC Biotechnol Volume: 12 Pages: 1-1
  • Peer Reviewed
• [Journal Article] H+/myo-inositol transporter genes, hmit-1.1 and hmit-1.2, have roles in the osmoprotective response in Caenorhabditis elegans (2011)
  • Author(s): Kage-Nakadai E, Uehara T, Mitani S
  • Journal Title: Biochemical Biophysical Research Communications Volume: 410 Pages: 471-477
• [Journal Article] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division (2011)
  • Author(s): Nomura, K.H., Murata, D., Hayashi, Y., Dejima, K., Mizuguchi, S., Kage-Nakadai, E., Gengyo-Ando, K., Mitani, S., Hirabayashi, Y., Ito, M., Nomura, K
  • Journal Title: Glycobiology Volume: 21 Pages: 834-848
• [Journal Article] H+/myo-inositol transporter genes, hmit-1.1 and hmit-1.2, have roles in the osmoprotective response in Caenorhabditis elegans. (2011)
  • Author(s): Kage-Nakadai E, Uehara T, Mitani S
  • Journal Title: Biochem Biophys Res Commun Volume: 410 Pages: 471-477
  • Peer Reviewed
• [Journal Article] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division. (2011)
  • Author(s): Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K
  • Journal Title: Glycobiology Volume: 21 Pages: 834-848
  • Peer Reviewed
• [Presentation] A conditional knockout system based on the single/low-copy integration of transgenes in C. elegans (2013)
  • Author(s): Kage-Nakadai E, Imae R, Funatsu O, Hori S, Suehiro Y, Yoshina S
  • Organizer: 19^<th> International C.elegans meeting
  • Place of Presentation: LA, USA(Poster)
• [Presentation] Single / low-copy integration of transgenes by UV/TMP methods (2012)
  • Author(s): Kage-Nakadai E, Imae R, Funatsu O, Hori S, Suehiro Y, Yoshina S, Mitani
  • Organizer: 5th East Asia C.elegans meeting
  • Place of Presentation: Taipei, Taiwan(Poster)
• [Presentation] Single / low-copy integration of transgenes by UV/TMP methods (2012)
  • Author(s): Kage-Nakadai E, Imae R, Funatsu O, Hori S, Suehiro Y, Yoshina S, Mitani
  • Organizer: 5th East Asia C. elegans meeting
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 線虫C.elegans におけるグリア細胞による感覚ニューロンの発生機能の制御 (2012)
  • Author(s): 中台（鹿毛）枝里子、三谷昌平
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
• [Presentation] Low-copy integration of transgenes by TMP/UV methods (2011)
  • Author(s): Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, Yoshina S, Hori S, Mitani
  • Organizer: 18th International C.elegans meeting
  • Place of Presentation: LA, USA(Poster)
• [Presentation] 線虫C. elegansにおけるグリア運命決定因子prospero/prox1の役割 (2011)
  • Author(s): 中台枝里子、三谷昌平
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜(口演)
• [Presentation] 線虫C. elegansにおけるH^+/myo-イノシトールトランスポーターの浸透圧応答への関与 (2011)
  • Author(s): 中台枝里子、三谷昌平
  • Organizer: 第88回日本生理学会
  • Place of Presentation: 盛岡(シンポジウム)
• [Presentation] 線虫C. elegansにおけるグリア運命決定因子prospero/prox1の役割 (2011)
  • Author(s): 中台（鹿毛）枝里子、三谷昌平
  • Organizer: 第３４回日本神経科学大会
  • Place of Presentation: 横浜
• [Book] Developmental Genetics of Caenorhabditis elegans . In : Brenner's Encyclopedia of Genetics, 2nd edn. vol 2 (2013)
  • Author(s): Kage-Nakadai, E., Mitani, S(Maloy, S. and Hughes, K. (eds.))
  • Publisher: London : Academic press
• [Book] Academic press (2012)
  • Author(s): Kage-Nakadai E, Mitani S
  • Total Pages: 4368
  • Publisher: Brenner’s Online Encyclopedia of Genetics, 2nd edn.
• [Book] 第3節哺乳類以外のモデル動物第4項-線虫-モデル動物利用マニュアル<生物機能モデルの作製/新しいリソース・リサーチツール> (2011)
  • Author(s): 中台-鹿毛枝里子、三谷昌平(小幡裕一、城石俊彦、芹川忠夫、田中啓二、米川博通編集)
  • Publisher: エル・アイ・シー出版