The involvement of astrocytes in the synaptic formation

• Project/Area Number: 23790257
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: OGAWA YASUHIRO 明治薬科大学, 薬学部, 講師 (00531948)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 分子・細胞生理学 / ニューロン―グリア相互作用 / 神経科学 / 脳・神経 / シナプス / グリア細胞 / アストロサイト / イオンチャネル

Research Abstract

The purpose of this study was to analyze the involvement of astrocytes in the synaptic formation in brain. We established with success a visualization of synaptic formation and a mRNA collection from the target cells in vivo. Using analysis of microarray gene expression data, it was also found that Foxh1 gene, which interacts with SMAD signaling, and its target molecules were over-expressed in the astrocytes derived from the model mice.

Research Products

• [Journal Article] Three mechanisms assemble central nervous system nodes of Ranvier (2013)
  • Author(s): Susuki K, Chang KJ, Zollinger DR, Liu Y, Ogawa Y, Eshed-Eisenbach Y, Dours-Zimmermann MT, Oses-Prieto JA, Burlingame AL, Seidenbecher CI, Zimmermann DR, Oohashi T, Peles E, Rasband MN
  • Journal Title: Neuron Volume: 78 Pages: 469-482
  • Peer Reviewed
• [Journal Article] Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease (2013)
  • Author(s): Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K
  • Journal Title: PLoS ONE Volume: 8
  • Peer Reviewed
• [Journal Article] Three mechanisms assemble central nervous system nodes of Ranvier. (2013)
  • Author(s): Susuki K, Chang KJ, Zollinger DR, Liu Y, Ogawa Y, Eshed-Eisenbach Y, Dours-Zimmermann MT, Oses-Prieto JA, Burlingame AL, Seidenbecher CI, Zimmermann DR, Oohashi T, Peles E, Rasband MN.
  • Journal Title: Neuron Volume: 78 Issue: 3 Pages: 469-482
  • DOI: 10.1016/j.neuron.2013.03.005
  • Peer Reviewed
• [Journal Article] Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease. (2013)
  • Author(s): Ogawa Y, Tanaka M, Tanabe M, Suzuki T, Togawa T, Fukushige T, Kanekura T, Sakuraba H, Oishi K.
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: e55856-e55856
  • DOI: 10.1371/journal.pone.0055856
  • Peer Reviewed
• [Journal Article] A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly (2012)
  • Author(s): Galiano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN
  • Journal Title: Cell Volume: 149 Pages: 1125-1139
  • Peer Reviewed
• [Journal Article] A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly. (2012)
  • Author(s): Galiano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN.
  • Journal Title: Cell Volume: 149 Issue: 5 Pages: 1125-1139
  • DOI: 10.1016/j.cell.2012.03.039
  • Peer Reviewed
• [Journal Article] Schwann cell spectrins modulate peripheral nerve myelination (2011)
  • Author(s): Susuki K, Raphael AR, Ogawa Y, Stankewich MC, Peles E, Talbot WS, Rasband MN
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Pages: 8009-8014
  • Peer Reviewed
• [Presentation] ザンドホッフ病モデルマウス脳の早期異常を見つけました (2014)
  • Author(s): 小川泰弘、大石一彦
  • Organizer: 臨床遺伝学公開シンポジウム
  • Place of Presentation: 明治薬科大学総合教育研究棟フロネシス
  • Year and Date: 2014-03-14
• [Presentation] P0-Cre x Z/EGトランスジェニックマウスから作製したiPS細胞 (2014)
  • Author(s): 江東洗、小川泰弘、大石一彦
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター
• [Presentation] ザンドホッフ病モデルマウスにおいてグリオーシスが発症前に起きている (2014)
  • Author(s): 佐野貴文、小川泰弘、棲庭均、大石一彦
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター
• [Presentation] ザンドホッフ病モデルマウスから作製したiPS細胞 (2014)
  • Author(s): 田中真、小川泰弘、鈴木俊宏、兎川忠靖、棲庭均、大石一彦
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館
• [Presentation] Induced pluripotent stem cells generated from P0-Cre x Z/EG transgenic mice (2014)
  • Author(s): Eto A, Ogawa Y, Oishi K.
  • Organizer: 薬理学会
  • Place of Presentation: 仙台国際センター
• [Presentation] Astrogliosis begins at presymptomatic stages in a mouse model of Sandhoff di sease (2014)
  • Author(s): Sano T, Ogawa Y, Sakuraba H, Oishi K.
  • Organizer: 薬理学会
  • Place of Presentation: 仙台国際センター
• [Presentation] ザンドホッフ病モデルマウス由来iPS細胞はニューロンに分化しにくいことを見つけました (2013)
  • Author(s): 高田昂、小川泰弘、大石一彦
  • Organizer: 臨床遺伝学公開シンポジウム
  • Place of Presentation: 明治薬科大学総合教育研究棟フロネシス
  • Year and Date: 2013-03-05
• [Presentation] ザンドホッフ病モデルマウス由来iPS細胞から神経系細胞を作りました (2012)
  • Author(s): 小川泰弘、大石一彦
  • Place of Presentation: 明治薬科大学総合教育研究棟フロネシス
  • Year and Date: 2012-03-10