Analysis into terminal erythroid differentiation and trial for establishing human erythroid progenitor cell lines.

• Project/Area Number: 23790263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HIROYAMA Takashi 独立行政法人理化学研究所, 細胞材料開発室, 専任研究員 (50373361)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ES / iPS / 臍帯血 / 赤血球前駆細胞株 / 赤血球分化 / ＥＳ / ｉＰＳ

Research Abstract

In this study, some genes that seem to be regulators for erythroid terminal differentiation were identified. Culture method that can produce hematopoietic cells, especially erythroid cells, was also developed. In addition to those, human erythroid progenitor cell lines were established by introducing virus oncogenes by inducible expression vector. These cell lines can produce enucleated erythrocyte.

Research Products

• [Journal Article] Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. (2013)
  • Author(s): Kurita R, Suda N, Sudo K, Miharada K, Hiroyama T, Miyoshi H, Tani K, Nakamura Y.
  • Journal Title: PLoS One. Volume: 8(3)
  • Peer Reviewed
• [Journal Article] Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. (2013)
  • Author(s): Kurita R
  • Journal Title: PLoS One. Volume: 8(3):e59890. Issue: 3 Pages: e59890-e59890
  • DOI: 10.1371/journal.pone.0059890
  • Peer Reviewed
• [Journal Article] Plasticity of Cells and Ex Vivo Production of Red Blood Cells. (2011)
  • Author(s): Hiroyama T, Miharada K, Kurita R, Nakamura Y.
  • Journal Title: Stem Cells Int. Volume: 2011 Pages: 195780-195780
  • Peer Reviewed
• [Journal Article] Plasticity of Cells and Ex Vivo Production of Red Blood Cells (2011)
  • Author(s): Hiroyama T, Miharada K, Kurita R, Nakamura Y
  • Journal Title: Stem Cells International Volume: 2011 Pages: 1-8
  • DOI: 10.4061/2011/195780
  • Peer Reviewed
• [Presentation] A useful method able to identify the genes required for terminal erythroid differentiation (2012)
  • Author(s): Hiroyama T, Okada N, Kurita R, Nakamura Y.
  • Organizer: 41st Annual Scientific Meeting of the ISEH.
  • Place of Presentation: Amsterdam
• [Presentation] A USEFUL METHOD ABLE TO IDENTIFY THE GENES REQUIRED FOR TERMINAL ERYTHROID DIFFERENTIATION (2012)
  • Author(s): Hiroyama T
  • Organizer: 41st Annual Scientific Meeting of the ISEH
  • Place of Presentation: Amsterdam
• [Book] 目的別で選べる細胞培養プロトコール (2012)
  • Author(s): 中村幸夫、西條薫、寛山隆、他
  • Publisher: 羊土社