| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
To reveal the core brain mechanism of fever development, I have performed multifaceted analyses of prostaglandin EP3 receptor-expressing neurons in the preoptic area (EP3 neurons), a center for fever development. By creating a novel transgenic rat and viruses, I established a system that allows us to specifically transduce EP3 neurons with a reporter gene. Using this system, I transduced EP3 neurons with a membrane-targeted form of GFP to visualize their axon fibers, and thereby, successfully identified all the brain regions where EP3 neurons project their axons. Also, by illuminating EP3 neurons transduced with channelrhodopsin, I was able to change thermogenic activity of brown adipose tissue in anesthetized rats. I am currently doing electrophysiological analyses of EP3 neurons in brain slices.
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