Molecular analysis of immune regulation by estrogen signaling through the G-coupled receptor
Project/Area Number |
23790273
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
|
Research Institution | Azabu University |
Principal Investigator |
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | GPR30 / 自然免疫 / IL-6 / シグナル伝達 / NFkB / GPER / 炎症性サイトカイン |
Research Abstract |
Estrogen effects the immune response through ERalpha or ERbeta, but recently the involvement of GPR30 (G protein Coupled Receptor 30), a membrane-type estrogen receptor, in anti inflammatory response is reported. This study investigated the molecular mechanism about the regulation of inflammatory cytokines by GPR30. We found that the treatment of GPR30 by G-1, GPR30 specific agonist, suppressed the expression of inflammatory cytokine IL-6 in macrophage cell line stimulated with LPS. G-1 also decreased NFkB promoter activation by LPS, and phosphorylation of kinases which involved in the translocation of transcription factor NFkB from cytosol to nucleus.
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Report
(4 results)
Research Products
(8 results)