Visualization of circadian rhythm in suprachiasmatic nucleus usinggreen fluorescent protein transgenic rats

• Project/Area Number: 23790280
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: The University of Tokushima
• Principal Investigator: FUJIHARA Hiroaki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (10369051)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: eGFP / 視交叉上核 / バゾプレッシン / サーカディアンリズム / 視交叉娘核

Research Abstract

I examined the effect of LED light stimulus to the suprachiasmatic nucleus (SCN) and the paraventricular nucleus (PVN) in mouse. The Fos-ir cells were increased in the SCN and the PVN after blue LED light stimulus. I investigated the gene expression in jet lag model using arginine vasopressin (AVP)-eGFP transgenic rats. The Per3gene is increased in the SCN in the jet lag AVP-eGFP transgenic rats.

Research Products

• [Journal Article] Expression of the c-fos-monomeric red fluorescent protein l fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after formalin injection (2012)
  • Author(s): Ishikura, T. Suzuki, H. Yoshimura, M. Ohkubo, J. Katoh, A. Ohbuchi, T. Ohno, M.Fujihara, H. Kawasaki, M. Ohnishi,H. Nakamura, T. & Ueta, Y
  • Journal Title: Brain Research Volume: 1479 Pages: 52-61
  • DOI: 10.1016/j.brainres.2012.08.033
  • Peer Reviewed
• [Journal Article] Water deprivation induces appetite and alters metabolic strategy in Notomys alexis: unique mechanisms for water production in the desert (2012)
  • Author(s): Takei, Y. Bartolo, R. Fujihara, H. Ueta, Y. & Donald, J
  • Journal Title: Proceedings of the Royal Society Volume: B24(4) Issue: 1738 Pages: 539-553
  • DOI: 10.1098/rspb.2011.2627
  • Peer Reviewed
• [Journal Article] Molecular characterization and biological function of neuroendocrine regulatory peptide-3 in the rat (2012)
  • Author(s): Fujihara, H. Sasaki, K. Mishiro-Sato, E.Ohbuchi, T. Dayanithi, G. Yamasaki, M. Ueta, Y. & Minamino, N
  • Journal Title: Endocrinology Volume: 153(3) Issue: 3 Pages: 1377-1386
  • DOI: 10.1210/en.2011-1539
  • Peer Reviewed
• [Journal Article] Induction of arginine vasopressin-enhanced green fluorescent protein expression in the locus coeruleus following kainic acid-induced seizures in rats (2012)
  • Author(s): Ohno, M. Fujihara, H. Iwanaga, M. Todoroki, M. Katoh, A. Ohbuchi, T. Ishikura, T. Hamamura, A. Hachisuka, K. & Ueta, Y
  • Journal Title: Stress Volume: 15(4) Issue: 4 Pages: 435-442
  • DOI: 10.3109/10253890.2011.637185
  • Peer Reviewed
• [Journal Article] Upregulation of arginine vasopressin synthesis in the rat hypothalamus after kainic acid-induced seizures (2011)
  • Author(s): Iwanaga, M.Ohno, M.Katoh, A.Ohbuchi, T.Ishikura, T.Fujihara, H.Nomura, M.Hachisuka, K., Ueta, Y.
  • Journal Title: Brain Research Volume: 1424 Pages: 1-8
  • DOI: 10.1016/j.brainres.2011.09.030
  • Peer Reviewed
• [Journal Article] Highly visible expression of an oxytocin-monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh, A. Eujihara, H. Ohbuchi, T. Onaka, T. ashimoto, T. Kawata, M. Suzuki, H. & Ueta, Y
  • Journal Title: Endocrinology, (Cover article of the issue) Volume: 152(7) Issue: 7 Pages: 2768-2774
  • DOI: 10.1210/en.2011-0006
  • Peer Reviewed
• [Journal Article] Possible contribution of pannexin channel to ATP-induced currents in vitro in vasopressin neurons isolated from the rat supraoptic nucleus (2011)
  • Author(s): Ohbuchi, T. Yokoyama, T. Saito, T. Ohkubo, J. Suzuki, H. Ishikura, T. Katoh, A. Fujihara, H. Hashimoto, H. Suzuki, H. & Ueta, Y
  • Journal Title: Brain Research Volume: 1394 Pages: 71-78
  • DOI: 10.1016/j.brainres.2011.04.017
  • Peer Reviewed
• [Journal Article] Angiotensin II type 1 receptor signaling regulates feeding behavior through anorexigenic corticotropin-releasing hormone in hypothalamus (2011)
  • Author(s): Yamamoto, R. Akazawa, H. Fujihara, H. Ozasa, Y. Yasuda, N. Ito, K. Kudo, Y. Qin, Y. Ueta, Y. & Komuro, I
  • Journal Title: Journal of Biological Chemistry Volume: 286(24) Issue: 24 Pages: 21458-21465
  • DOI: 10.1074/jbc.m110.192260
  • Peer Reviewed
• [Presentation] Neuroendocrine regulatory peptide-3 のラット視床下部における分布と生理機能 (2013)
  • Author(s): 藤原広明
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本市総合体育館(長野県)
  • Year and Date: 2013-03-31
• [Presentation] 摂食リズムが視床下部性代謝調節に及ぼす影響 (2013)
  • Author(s): 志内哲也
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 2013-03-29
• [Presentation] 摂食リズムが視床下部性代謝調節に及ぼす影響 (2013)
  • Author(s): 志内哲也
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀（東京都）
• [Presentation] Neuroendocrine regulatory peptide!3 のラット視床下部における分布と生理機能 (2012)
  • Author(s): 藤原広明
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本市総合体育館（長野県）