Functional analysis of the zinc transporter involved in male fertility.

• Project/Area Number: 23790284
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: FUKUMURA Ryutaro 独立行政法人理化学研究所, 新規変異マウス研究開発チーム, 開発研究員 (90392240)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 亜鉛トランスポーター / 雄性不妊 / ENU / 男性不妊

Research Abstract

This study revealed that murine zinc transporter Slc39a6 gene was essential for male fertility. The ENU mutant mice that carried a nonsense mutation on Slc39a6 gene exhibited sterility in male. The testis and spermatozoa morphologies of Slc39a6 mutant mice were normal. By in vitro fertilization, the decreased fertility of their spermatozoa was observed. Slc39a6 gene may act to the sperm fertility.

Research Products

• [Journal Article] Comprehensive gene expression analyses in pluripotent stem cells of a planarian (2012)
  • Author(s): Shibata N, Fukumura R
  • Journal Title: Dugesia japonica, Int J Dev Biol Volume: 56 Pages: 93-102
  • Peer Reviewed
• [Journal Article] Mouse mutagenesis and disease models for neuropsychiatric disorders. (2011)
  • Author(s): Gondo Y, Fukumura R
  • Journal Title: Curr Topics in Behavioral Neurosci Volume: 7 Pages: 1-35
  • Peer Reviewed
• [Journal Article] Missense mutation of the reticulon-4 receptor alters spatial memory and social interaction in mice. (2011)
  • Author(s): Lazar N, Gondo Y, Fukumura R
  • Journal Title: Behavioural Brain Research Volume: 224 Pages: 73-79
  • Peer Reviewed
• [Presentation] MassARRAYを用いたマウスエクソーム解析からの点突然変異候補の検証実験 (2012)
  • Author(s): 福村龍太郎
  • Organizer: 第35回日本分子生物学会年回
  • Place of Presentation: 福岡・福岡国際会議場
• [Presentation] Novel mutation discovery on ENU mouse mutagenesis by exome targeted re-sequencing (2011)
  • Author(s): Fukumura R, Gondo Y et al.
  • Organizer: Mouse Genetics 2011
  • Place of Presentation: Washington, DC USA 国際会議
  • Year and Date: 2011-06-24
• [Presentation] Assessment of exome resequencing by NGS to detect genomewide heterozygous point mutations in the mouse (2011)
  • Author(s): Fukumura R, Gondo Y et al.
  • Organizer: 9th Int'l Workshop on Advanced Genomics (AGW)
  • Place of Presentation: Tokyo Japan 国際会議
• [Presentation] マウスエクソーム解析を用いたENUマウスゲノム上の点突然変異の網羅的同定 (2011)
  • Author(s): 福村龍太郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Novel mutation discovery on ENU mouse mutagenesis by exome targeted re-sequencing (2011)
  • Author(s): 福村龍太郎
  • Organizer: Mouse Genetics 2011
  • Place of Presentation: ワシントンDC（アメリカ）