Analysis for mechanisms of neurotransmitter transport via Dysbindin

• Project/Area Number: 23790291
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Nagoya University
• Principal Investigator: IIZUKA Yukihiko 名古屋大学, 医学系研究科, 研究員 (00365404)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 精神神経疾患 / 統合失調症 / 脆弱性遺伝子

Research Abstract

Although the etiological mechanisms of psychiatric disorders, such as schizophrenia, remain largely esoteric, multiple hypotheses (e.g. dysfunction of dopamine, glutamate or serotonin, neurodevelopmental disorders, stress during pregnancy and viral infection) have been proposed. The dissection of molecular pathways centered on promising risk factors, such as Disrupted-In-Schizophrenia 1 (DISC1), may help us achieve a better understanding of the pathogenesis of these disorders. In this study, I identified a novel interacting protein complex, V-ATPase, as a partner of DISC1. I found significant protein reduction of a couple of V-ATPase subunits in synaptic vesicle fractions isolated from the DISC1-deficient mice compare to proteins isolated from the wildtype mice.

Research Products

• [Journal Article] 統合失調症の動物モデル (2012)
  • Author(s): 黒田啓介, 飯塚幸彦, 貝淵弘三
  • Journal Title: 実験医学増刊号 in vivo実験医学によるサイエンスと疾患解明 Volume: 30 Pages: 231-235
• [Journal Article] Behavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouse (2011)
  • Author(s): K Kuroda, S Yamada, M Tanaka, M Iizuka, H Yano, D Mori, D Tsuboi, T Nishioka, T Namba, Y Iizuka, S Kubota, T Nagai, D Ibi, R Wang, A Enomoto, M Isotani-Sakakibara, N Asai, K Kimura, H Kiyonari, T Abe, A Mizoguchi,M Sokabe, M Takahashi, K Yamada, K Kaibuchi
  • Journal Title: Human molecular genetics Volume: 20 Issue: 23 Pages: 4666-4683
  • DOI: 10.1093/hmg/ddr400
  • Peer Reviewed
• [Presentation] Comprehensive proteomic analysis reveals novel binging partners of DISC1, a candidate risk gene of Schizophrenia (2012)
  • Author(s): Iizuka Y, Sharmin A, Tsuboi D, Mori D, Kuroda K, Nishioka T, and Kaibuchi K
  • Organizer: 第44回日本神経学会大会
  • Place of Presentation: 名古屋
• [Presentation] Comprehensive proteomic analysis reveals novel binging partners of DISC1, a candidate risk gene of Schizophrenia. (2012)
  • Author(s): Yukihiko Iizuka
  • Organizer: 第44回日本神経学会大会
  • Place of Presentation: 名古屋
• [Presentation] Proteomic analysis reveals novel binging partner of DISC1, a candidate risk gene of Schizophrenia (2011)
  • Author(s): Iizuka Y, Kinoshita T, Tsuboi D, Mori D, Kuroda K, and Kaibuchi K
  • Organizer: 第43回日本神経学会大会
  • Place of Presentation: 横浜
• [Presentation] Proteomic analysis reveals novel binging partner of DISC1, a candidate risk gene of Schizophrenia. (2011)
  • Author(s): Iizuka Y, Kinoshita T, Tsuboi D, Mori D, Kuroda K, and Kaibuchi K
  • Organizer: 第43回日本神経学会大会
  • Place of Presentation: パシフィコ横浜（神奈川）
• [Book] 統合失調症の動物モデル (2012)
  • Author(s): 黒田啓介, 飯塚幸彦, 貝淵弘三
  • Publisher: 羊土社
• [Book] 実験医学増刊号 in vivo実験医学によるサイエンスと疾患解明 「統合失調症の動物モデル」 (2012)
  • Author(s): 黒田啓介
  • Publisher: 羊土社