Investigation of TBK1 activation mechanism

• Project/Area Number: 23790296
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Nara Institute of Science and Technology (2013); Osaka University (2011-2012)
• Principal Investigator: KAWASAKI Takumi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60584414)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ホスファチジルイノシトール / 自然免疫 / シグナル伝達 / リン酸化 / ウイルス / イノシトールリン脂質 / PIKfyve / ホスファチジルイノシトール５リン酸 / セリンスレオニンキナーゼ / ウィルス感染 / スクリーニング / TBK1 / IRF3

Research Abstract

TBK1 (TANK Binding Kinase 1) is important gene for host defence against virus or microbes infection. Activation mechanism for TBK1 is still unclear. Here we investigated activation mechanism by screening cellular component, and found that Ptdins5P works as signaling mediator for innate immune response during virus infection. Furthermore, synthetic Ptdins5P immunization with antigen induced antigen specific antibody production by TBK1 signaling activation.

Research Products

• [Journal Article] The second messenger phosohatidylinositol-5-phosphate facilitates antiviral innate immune signaling (2013)
  • Author(s): Kawasaki T, Takemura N, Standley M D, Akira S, Kawai T
  • Journal Title: Cell Host Microbe Volume: 14 Pages: 148-158
  • Peer Reviewed
• [Journal Article] The second messenger phosohatidylinositol-5-phosphate facilitates antiviral innate immune signaling (2013)
  • Author(s): Kawasaki T, Takemura N, Standley M D, Akira S, Kawai T.
  • Journal Title: Cell Host Microbe. Volume: 14 Pages: 148158-148158
  • Peer Reviewed
• [Presentation] PtdIns5P works as a new type of adjuvant (2013)
  • Author(s): 川﨑拓実、武村直紀、Daron M Standley、審良静男、河合太郎
  • Organizer: 第42回日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] PtdIns5P works as a new type of adjuvant (2013)
  • Author(s): Kawasaki T, Takemura N, Standley M D, Akira S, Kawai T
  • Organizer: 日本免疫学会
  • Place of Presentation: 幕張メッセ
• [Presentation] Identification and characterization of a cellular component that modulate TBK1-IRF3 signal axis (2012)
  • Author(s): 川﨑拓実、武村直紀、Daron M Standley、審良静男、河合太郎
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] Identification and characterization of a cellular component that modulates TBK1-IRF3 signal axis (2012)
  • Author(s): 川崎 拓実
  • Organizer: 第４１回日本免疫学会
  • Place of Presentation: 神戸国際会議場
• [Book] ライフサイエンス新着レビューホスファチジルイノシトール5リン酸による自然免疫応答の制御 (2013)
  • Author(s): 川﨑拓実、審良静男、河合太郎
  • Publisher: ライフサイエンス統合データベースセンター
  • URL: http://first.lifesciencedb.jp/archives/7606
• [Book] ホスファチジルイノシトール５リン酸による自然免疫応答の制御 (2013)
  • Author(s): 川﨑拓実、審良静男、河合太郎
  • Total Pages: 4
  • Publisher: ライフサイエンス新着レビュー
• [Remarks]
  • URL: http://bsw3.naist.jp/courses/courses209.html
• [Patent(Industrial Property Rights)] アジュバント及びそれを含むワクチン (2012)
  • Inventor(s): 川崎 拓実
  • Industrial Property Rights Holder: 川崎 拓実
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-10-22