Elucidation of the pathogensis of aortic aneurysm and dissection by the use of mice lacking all nitric oxide synthases.

• Project/Area Number: 23790300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: University of the Ryukyus
• Principal Investigator: ARAKAKI Kumiko 琉球大学, 医学部附属病院, 助教 (10568687)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 一酸化窒素合成酵素 / 大動脈瘤 / 大動脈解離 / マウス / 一酸化窒素 / メタボリックシンドローム / 動脈硬化

Research Abstract

The pathogesisis of aortic aneurysm and dissection still remains to be fully clarified. In this study, we examined whether or not the nitric oxide synthases(NOSs) system is involved in the pathogenesis of aortic aneurysm and dissection by using triple NOSs-KO mice. Long-term treatment with a high-fat diet and angiotensinII did not induced aortic aneurysm or dissection in the triple NOSs-KO mice. There may be room for considering experimental conditions.

Research Products

• [Journal Article] Long-Term Treatment With San’o-Shashin-To, a Kampo Medicine, Markedly Ameliorates Cardiac Ischemia-Reperfusion Injury in Ovariectomized Rats via the Redox-Dependent Mechanism (2013)
  • Author(s): Sakanashi M, Matsuzaki T, Noguchi K, Nakasone J, Sakanashi M, Uchida T, Kina-Tanada M, Kubota H, Arakaki K, Tanimoto A, Yanagihara N, Sakanashi M, Ohya Y, Masuzaki H, Ishiuchi S, Sugahara K, Tsutsui M
  • Journal Title: Circulation Journal Volume: 77 Issue: 7 Pages: 1827-1837
  • DOI: 10.1253/circj.CJ-12-1434
  • NAID: 10031173765
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Increasing dihydrobiopterin causes dysfunction of endothelial nitric oxide synthase in rats in vivo (2011)
  • Author(s): Noguchi K, Hamadate N, Matsuzaki T, Sakanashi M, Nakasone J, Uchida T, Arakaki K, Kubota H, Ishiuchi S, Masuzaki H, Sugahara K, Ohya Y, Sakanashi M, Tsutsui M
  • Journal Title: Am J Physiol Heart Circ Physiol Volume: 301 Issue: 3 Pages: H721-H729
  • DOI: 10.1152/ajpheart.01089.2010
  • Peer Reviewed
• [Presentation] Development of an Experimentally Useful Model of Spontaneous Myocardial Infarction : 2/3 Nephrectomized Triple Nitric Oxide Synthases-Deficient Mouse (2014)
  • Author(s): Uchida T, Furuno Y, Tanimoto H, Arakaki K, Kina M, Kubota H, Sakanashi M, Matsuzaki T, Noguchi K, Nakasone J, Ohya Y, Yanagihara N, Shimokawa H, Tamura M, Otsuji Y, Tsutsui M
  • Organizer: The 78th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Tokyo International Forum, Tokyo, Japan
  • Year and Date: 2014-03-22
• [Presentation] 実験に有用な自然発症 急性心筋梗塞モデルの開発 (2014)
  • Author(s): 内田太郎、古野由美、新垣久美子、喜名美香、久保田陽秋、坂梨まゆ子、松崎俊博、野口克彦、仲宗根淳子、大屋祐輔、柳原延章、下川宏明、田村雅仁、尾辻豊、筒井正人
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター. 仙台
  • Year and Date: 2014-03-19
• [Presentation] Dihydrobiopterin causes dysfunction of endothelial nitric oxide synthase in rats in vivo (2012)
  • Author(s): Noguchi K, Matsuzaki T, Sakanashi M, Nakasone J, Arakaki K, Sakanashi M, Ohya Y, Tsutsui M
  • Organizer: The 76th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Fukuoka Kokusai Center, Fukuoka, Japan
  • Year and Date: 2012-03-18
• [Presentation] 三黄瀉心湯はMn-SOD の選択的な活性化を介して卵巣摘出ラットの虚血再灌流心機能障害を軽減する (2012)
  • Author(s): 坂梨まゆ子、野口克彦、松﨑俊博、仲宗根淳子、新垣久美子、内田太郎、坂梨真木子、久保田陽秋、棚田美香、大屋祐輔、須加原一博、坂梨又郎、筒井正人
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館. 京都
  • Year and Date: 2012-03-15
• [Presentation] 血管内におけるジヒドロビオプテリン増加はeNOS 機能障害を引き起こす (2012)
  • Author(s): 野口克彦、松﨑俊博、坂梨まゆ子、濱舘直史、仲宗根淳子、新垣久美子、坂梨又郎、筒井正人
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館. 京都
  • Year and Date: 2012-03-14
• [Presentation] 内皮型NO 合成酵素(eNOS)の新しい活性調節機構 : ラット血管内Dihydrobiopterin (BH2)増加によるeNOS機能障害 (2012)
  • Author(s): 筒井正人、野口克彦、松崎俊博、坂梨まゆ子、仲宗根淳子、内田太郎、新垣久美子、久保田陽秋、須加原一博、大屋祐輔
  • Organizer: 第41回日本心脈管作動物質学会
  • Place of Presentation: 秋田キャッスルホテル. 秋田
  • Year and Date: 2012-02-10
• [Presentation] 内皮型NO 合成酵素の新しい活性調節機構 : ラット血管内Dihydrobiopterin(BH2)増加によるeNOS uncoupling の惹起 (2011)
  • Author(s): 筒井正人、野口克彦、松崎俊博、坂梨まゆ子、仲宗根淳子、内田太郎、新垣久美子、久保田陽秋、須加原一博、大屋祐輔
  • Organizer: 第64回日本薬理学会西南部会
  • Place of Presentation: KKR ホテル博多. 福岡
  • Year and Date: 2011-11-20
• [Presentation] Increasing dihydrobiopterin causes dysfunction of endothelial nitric oxide synthase in rats in vivo (2011)
  • Author(s): Tsutsui M, Noguchi K, Matsuzaki T, Sakanashi M, Nakasone J, Uchida T, Arakaki K, Kubota H, Sugahara K, Ohya Y
  • Organizer: American Heart Association, Scientific Sessions 2011
  • Place of Presentation: Orange County Convention Center, Orlando, USA
  • Year and Date: 2011-11-16