The role of aquaporin in the intestine in water transport

• Project/Area Number: 23790306
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: Hoshi University
• Principal Investigator: IKARASHI Nobutomo 星薬科大学, 助教 (40409363)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: アクアポリン / 下痢 / 便秘 / 大腸 / 下剤 / 腸管

Research Abstract

We investigated the role of aquaporin-3 (AQP3) in the intestine in water transport.In this study, it has been revealed that the fecal water content in the colon is controlled by the transport of water from the luminal side to the vascular side, which is mediated by AQP3. Thus, new laxatives and antidiarrheals targeting intestinal AQP3 may be developed in the future.

Research Products

• [Journal Article] The concomitant use of an osmotic laxative, magnesium sulphate, and a stimulant laxative, bisacodyl, does not enhance the laxative effect (2012)
  • Author(s): N.Ikarashi, A.Mimura, R.Kon, T.Iizasa, M.Omodaka, C.Nagoya, M.Ishii, T.Toda, W.Ochiai, and K.Sugiyama.
  • Journal Title: Eur. J. Pharm. Sci. Volume: 45 Pages: 73-78
• [Journal Article] Ochiai, and K. Sugiyama. Inhibition of aquaporin-3 water channel in the colon induces diarrhea (2012)
  • Author(s): N.Ikarashi, R.Kon, T.Iizasa, N.Suzuki, R.Hiruma, K.Suenaga, T.Toda, M.Ishii, M.Hoshino, W.
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 957-962
• [Journal Article] Inhibition of aquaporin-3 water channel in the colon induces diarrhea (2012)
  • Author(s): N. Ikarashi
  • Journal Title: Biol. Pharm. Bull. Volume: 35 Pages: 957-962
  • NAID: 130001872180
  • Peer Reviewed
• [Journal Article] The laxative effect of bisacodylis attributable to decreased aquaporin-3 expression in the colon induced by increased PGE_2 secretion from macrophages (2011)
  • Author(s): N.Ikarashi, K.Baba, T.Ushiki, R.Kon, A.Mimura, T.Toda, M.Ishii, W.Ochiai, and K.Sugiyama.
  • Journal Title: Am. J. Physiol. Gastrointest. Liver Volume: 301
• [Journal Article] Effects of magnesium sulphate administration on aquaporin 3 in rat gastrointestinal tract (2011)
  • Author(s): N.Ikarashi, T.Ushiki, T.Mochiduki, T.Toda, T. Kudo, K.Baba, M.Ishii, K.Ito, W.Ochiai, and K.Sugiyama.
  • Journal Title: Biol. Pharm. Bull. Volume: 34 Pages: 238-242
  • NAID: 130000402196
• [Journal Article] Effects of magnesium sulphate administration on aquaporin 3 in rat gastrointestinal tract (2011)
  • Author(s): Ikarashi N., Ushiki T., Mochiduki T., Toda T., Kudo T., Baba K., Ishii M., Ito K., Ochiai W., Sugiyama K.
  • Journal Title: Biol. Pharm. Bull Volume: 34 Pages: 238-242
  • NAID: 130000402196
  • Peer Reviewed
• [Journal Article] The laxative effect of bisacodyl is attributable to decreased aquaporin-3 expression in the colon induced by increased PGE2 secretion from macrophages (2011)
  • Author(s): Ikarashi N., Baba K., Ushiki T., Kon R., Mimura A., Toda T., Ishii M., Ochiai W., Sugiyama K.
  • Journal Title: Am. J. Physiol. Gastrointest. Liver Volume: 301
  • Peer Reviewed
• [Journal Article] A mechanism by which the osmotic laxative magnesium sulphate increases the intestinal aquaporin 3 expression in HT-29 cells (2010)
  • Author(s): N.Ikarashi, T.Mochiduki, A.Takasaki, T.Ushiki, K. Baba, M.Ishii, T.Kudo, K.Ito, T.Toda, W. Ochiai, and K.Sugiyama
  • Journal Title: Life Sci. Volume: 88 Pages: 194-200
• [Presentation] 大腸におけるアクアポリン3の機能解析とその発現制御機構の解明 (2012)
  • Author(s): 五十嵐信智
  • Organizer: 第56回日本薬学会関東支部大会
  • Place of Presentation: 東京
  • Year and Date: 2012-10-13
• [Presentation] 浸透圧性下剤硫酸マグネシウムと大腸刺激性下剤ビサコジルを併用しても、瀉下作用は増強しない (2012)
  • Author(s): 五十嵐信智,今理紗子,三村綾子,飯笹朋彦,面高みどり,名古屋智香,石井敬,戸田雄大,落合和,杉山清
  • Organizer: 医療薬学フォーラム2012
  • Place of Presentation: 福岡
• [Presentation] 大腸のアクアポリン3の活性が低下すると下痢が発症する (2012)
  • Author(s): 今理紗子,五十嵐信智,楠欣己,石井敬,落合和,杉山清
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡
• [Presentation] ビサコジルは大腸マクロファージからのPGE2の分泌亢進を介して、大腸粘膜上皮細胞のアクアポリン3の発現を低下させることにより下痢を引き起こす (2012)
  • Author(s): 五十嵐信智,今理紗子,楠欣己,石井敬,落合和,杉山清
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡
• [Presentation] ビサコジルによる腸管アクアポリン3の発現変化およびそのメカニズムの解明 (2012)
  • Author(s): 今理紗子、五十嵐信智、馬場弘太、宇敷 隆、三村綾子、楠 欣己、戸田雄大、石井 敬、落合 和、杉山 清
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌