Production, release and neuroprotective effect of hydrogen sulfide

• Project/Area Number: 23790316
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General pharmacology
• Research Institution: The University of Tokyo (2012); National Center of Neurology and Psychiatry (2011)
• Principal Investigator: MIKAMI Yoshinori 東京大学, 大学院・医学系研究科, 特任研究員 (80532671)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 硫化水素 / カルシウム / ガスメディエータ / 細胞保護 / 神経細胞 / 網膜 / アストロサイト / 生理活性物質 / 薬理学

Research Abstract

Hydrogen sulfide (H2S) is produced by 3-mercaptopyruvate sulfurtransferase (3MST) and cysteine aminotransferase (CAT) in the neurons. Thioredoxin and dihydrolipoic acid are required for 3MST to produce H2S. The production of H2S is regulated by calcium (Ca2+); H2S, in turn, regulates Ca2+ influx into photoreceptor cells. H2S protects retinal neurons from light-induced degeneration. H2S can be applied for therapeutic use.

Research Products

• [Journal Article] Polysulfides are possible H_2S-derived signaling molecules in rat brain. (2013)
  • Author(s): Yuka Kimura, Yoshinori Mikami, Kimiko Osumi, Mamiko Tsugane, Junichiro Oka, Hideo Kimura
  • Journal Title: FASEB Journal Volume: 27 Issue: 6 Pages: 2451-2457
  • DOI: 10.1096/fj.12-226415
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide is produced by cystathionine gamma-lyase at the steady-state low intracellular Ca2+ concentrations (2013)
  • Author(s): Mikami Y, Shibuya N, Ogasawara Y, Kimura H.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 431 Issue: 2 Pages: 131-135
  • DOI: 10.1016/j.bbrc.2013.01.010
  • Peer Reviewed
• [Journal Article] A mechanism of retinal protection from light-induced degeneration by hydrogen sulfide (2012)
  • Author(s): Mikami, Y., and Kimura, H
  • Journal Title: Commun Integr. Biol Volume: 5 Issue: 2 Pages: 169-171
  • DOI: 10.4161/cib.18679
  • Peer Reviewed
• [Journal Article] A mechanism of retinal protection from light-induced degeneration by hydrogen sulfide (2012)
  • Author(s): Mikami, Y. and Kimura, H.
  • Journal Title: Communicative & Integrative Biology Volume: 5 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Development of a highly selective fluorescence probe for hydrogen sulfide (2011)
  • Author(s): Sasakura K, Hanaoka K, Shibuya N, Mikami Y, Kimura Y, Komatsu T, Ueno T, Terai T, Kimura H, Nagano T
  • Journal Title: Journal of the American Chemical Society Volume: 133(45) Issue: 45 Pages: 18003-18005
  • DOI: 10.1021/ja207851s
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide protects the retina from light-induced degeneration by the modulation of Ca2+ influx (2011)
  • Author(s): Mikami Y, Shibuya N, Kimura Y, Nagahara N, Yamada M, Kimura H
  • Journal Title: J Biol Chem. Volume: 286(45) Issue: 45 Pages: 39379-86
  • DOI: 10.1074/jbc.m111.298208
  • Peer Reviewed
• [Journal Article] Protein tyrosine phosphataseσregulates the synapse number of zebrafish olfactory sensory neurons (2011)
  • Author(s): Chen, X., Yoshida, T., Sagara, H., Mikami, Y. and Mishina, M
  • Journal Title: J. Neurochem Volume: 119 Issue: 3 Pages: 532-543
  • DOI: 10.1111/j.1471-4159.2011.07411.x
  • Peer Reviewed
• [Journal Article] Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide (2011)
  • Author(s): Mikami Y, Shibuya N, Kimura Y, Nagahara N, Ogasawara Y, Kimura H
  • Journal Title: Biochem J. Volume: 1;439(3) Issue: 3 Pages: 479-85
  • DOI: 10.1042/bj20110841
  • Peer Reviewed
• [Presentation] Production of H2Sand its protection of retinal neurons fromlight-induced degeneration. (2013)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、永原則之、山田雅弘.
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2013-03-22
• [Presentation] Cytoprotective effect of hydrogen sulfide on light-induced retinal degeneration. (2013)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則 之、山田雅弘、木村英雄.
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都府).
  • Year and Date: 2013-03-16
• [Presentation] Production of H2S and its protection of retinal neurons from light-induced degeneration (2013)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、小笠原裕樹、永原則之、山田雅弘
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡国際会議場（福岡県）
• [Presentation] Regulation of H2S production by 3-mercaptopyruvate sulfurtransferase. (2012)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由 佳、小笠原裕樹、永原則之.
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・ マリンメッセ福岡(福岡県).
  • Year and Date: 2012-12-16
• [Presentation] Hydrogen sulfide(H2S) protects retinal photoreceptor cells from light-induced degeneration. (2012)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則 之、山田雅弘、木村英雄.
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場(愛知県).
  • Year and Date: 2012-09-20
• [Presentation] Thioredoxin and dihydrolipoic acid are required for3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide. (2012)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由 佳、永原則之、小笠原裕樹.
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都府).
  • Year and Date: 2012-03-15
• [Presentation] Hydrogen sulfide (H2S) protects retinal photoreceptor cells from light-induced degeneration (2012)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則之、山田雅弘、木村英雄
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Regulation of H2S production by 3-mercaptopyruvate sulfurtransferase (2012)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、小笠原裕樹、永原則之
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide (2012)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、永原則之、小笠原裕樹
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] Cytoprotective effect of hydrogen sulfide on light-induced retinal degeneration (2012)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則之、山田雅弘、木村英雄
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] 3-メルカプトピルビン酸硫黄転移酵素の硫化水素生産補因子. (2011)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由 佳、小笠原裕樹.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府).
  • Year and Date: 2011-09-23
• [Presentation] 網膜における 硫化水素の生合成と細胞保護作用. (2011)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則 之、山田雅弘、木村英雄.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府).
  • Year and Date: 2011-09-22
• [Presentation] Endogenous reductants required for 3MST to produce H2S. (2011)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由 佳、小笠原裕樹.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県).
  • Year and Date: 2011-09-17
• [Presentation] Regulation of hydrogen sulfide production and its cytoprotective effect in the retina. (2011)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則 之、山田雅弘、木村英雄.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県).
  • Year and Date: 2011-09-16
• [Presentation] Regulation of synapse number by presynaptic PTPσ in zebrafish olfactory sensory neurons. (2011)
  • Author(s): 陳西貴、吉田知之、相良洋、三上義礼、 三品昌美.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県).
  • Year and Date: 2011-09-15
• [Presentation] Regulation of synapse number by protein tyrosine phosphatase σ. The 4th Molecular and Cellular (2011)
  • Author(s): Xigui Chen, Tomoyuki Yoshida, Hiroshi Sagara, Yoshinori Mikami and Masayoshi Mishina.
  • Organizer: Cognition Society-ASIA Symposium.
  • Place of Presentation: ソウル大韓民国.
• [Presentation] Regulation of hydrogen sulfide production and its cytoprotective effect in the retina (2011)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則之、山田雅弘、木村英雄
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Endogenous reductants required for 3MST to produce H2S (2011)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、小笠原裕樹
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 網膜における硫化水素の生合成と細胞保護作用 (2011)
  • Author(s): 三上義礼、渋谷典広、木村由佳、永原則之、山田雅弘、木村英雄
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館（京都府）
• [Presentation] 3-メルカプトピルビン酸硫黄転移酵素の硫化水素生産補因子 (2011)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、小笠原裕樹
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会館（京都府）
• [Remarks] 独立行政法人国立精神・神経医療研究センター神経研究所 神経薬理研究部
  • URL: http://www.ncnp.go.jp/nin/guide/r_dna/index.html
• [Remarks] 独立行政法人国立精神・神経医療研究センター プレスリリース
  • URL: http://www.ncnp.go.jp/press/press_release130311.html
• [Remarks] 東京大学大学院医学系研究科 細胞分子薬理学教室
  • URL: http://calcium.cmp.m.u-tokyo.ac.jp/index.html
• [Remarks]
  • URL: http://www.ncnp.go.jp/nin/guide/r_dna/index.html