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Function of Z-DNA structure in Nrf2-dependent gene activation

Research Project

Project/Area Number 23790319
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionHirosaki University

Principal Investigator

MARUYAMA Atsushi  弘前大学, 医学(系)研究科(研究院), 助教 (10431438)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords酸化ストレス / 転写制御 / Nrf2 / Z-DNA / ヘムオキシゲナーゼ‐1 / HO-1 / 転写調節
Research Abstract

Left-handed double helix of DNA, called Z-DNA, is an alternative structure of DNA and appears to have many diverse roles in nuclear processes such as transcriptional regulation and nucleosome positioning. In this study, I have developed a method for in vivo detection of Z-DNA formation and show Nrf2 activation is associated with Z-DNA formation in the HO-1 gene promoter. The results implicate Nrf2-dependent Z-DNA formation in HO-1 gene transcriptional activation and suggestthe involvement of BRG1 in Z-DNA formation. Human HO-1 gene promoter might be a good model system to understand Z-DNA formation and how Z-DNA forming sequences regulate geneexpression.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (22 results)

All 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (8 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Nrf2 activation is associated with Z-DNA formation in the human HO-1 promoter2013

    • Author(s)
      Maruyama A, Mimura J, Harada N, Itoh K
    • Journal Title

      Nucleic Acids Res

      Volume: (in press) Issue: 10 Pages: 12-12

    • DOI

      10.1093/nar/gkt243

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Keap1 is localized in neuronal and glial cytoplasmic inclusions in various neurodegenerative diseases2013

    • Author(s)
      Tanji K, Maruyama A, Odagiri S, Mori F, Itoh K, Kakita A, Takahashi H, Wakabayashi K
    • Journal Title

      J Neuropathol Exp Neurol

      Volume: 72 Issue: 1 Pages: 18-28

    • DOI

      10.1097/nen.0b013e31827b5713

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alteration of autophagosomal proteins in the brain of multiple system atrophy.2012

    • Author(s)
      Tanji K, Odagiri S, Maruyama A, Mori F, Kakita A, Takahashi H, Wakabayashi K.
    • Journal Title

      Neurobiol Dis

      Volume: 49C Pages: 190-198

    • DOI

      10.1016/j.nbd.2012.08.017

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nrf2 in bone marrow-derived cells positively contributes to the advanced stage of atherosclerotic plaque formation2012

    • Author(s)
      Harada N, Ito K, Hosoya T, Mimura J, Maruyama A, Noguchi N, Yagami KI, Morito N, Takahashi S, Maher JM, Yamamoto M, Itoh K
    • Journal Title

      Free Radic Biol Med

      Volume: 53 Issue: 12 Pages: 2256-2262

    • DOI

      10.1016/j.freeradbiomed.2012.10.001

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nrf2 regulates NGF mRNA induction by carnosic acid in T98G glioblastoma cells and normal human astrocytes2011

    • Author(s)
      Mimura J, Kosaka K, Maruyama A, Satoh T, Harada N, Yoshida H, Satoh K, Yamamoto M, Itoh K
    • Journal Title

      J Biochem

      Volume: 150 Issue: 2 Pages: 209-217

    • DOI

      10.1093/jb/mvr065

    • NAID

      10030587033

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The novel NRF2-interacting factor KAP1 regulates the susceptibility to oxidative stress by promoting NRF2-mediated cytoprotective response2011

    • Author(s)
      Maruyama A, Nishikawa K, Kawatani Y, Mimura J, Hosoya T, Harada N, Yamamato M, Itoh K
    • Journal Title

      Biechem J

      Volume: 436 Issue: 2 Pages: 387-397

    • DOI

      10.1042/bj20101748

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nrf2 regulates ferroportin 1-mediated iron efflux and counteracts lipopolysaccharide-induced ferroportin-1 mRNA suppression in macrophages2011

    • Author(s)
      Harada N, Kanayama M, Maruyama A, Yoshida A, Tazumi K, Hosoya T, Mimura J, Toki T, Maher JM, Yamamoto M, Itoh K
    • Journal Title

      Arch Biochem Biophys

      Volume: 508 Issue: 1 Pages: 101-109

    • DOI

      10.1016/j.abb.2011.02.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nrf2 regulates ferroportin 1-mediated iron efflux and counteracts lipopolysaccharide-induced ferroportin 1 mRNA suppression in macrophages.2011

    • Author(s)
      Harada N*, Kanayama M*, Maruyama A*, Yoshida A, Tazumi K, Hosoya T, Mimura J, Toki T, Maher JM, Yamamoto M, Itoh K.*: equal contribution.
    • Journal Title

      Arch. Biochem. Biophys.

      Volume: 508 Pages: 101-109

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The novel NRF2-interacting factor KAP1 regulates susceptibility to oxidative stress by promoting the NRF2-mediated cytoprotective response.2011

    • Author(s)
      Maruyama A, Nishikawa K, Kawatani Y, Mimura J, Hosoya T, Harada N, Yamamoto M, Itoh K.
    • Journal Title

      Biochem. J.

      Volume: 436 Pages: 387-397

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nrf2 regulates NGF mRNA induction by carnosic acid in T98G glioblastoma cells and normal human astrocytes.2011

    • Author(s)
      Mimura J, Kosaka K, Maruyama A, Satoh T, Harada N, Yoshida H, Satoh K, Yamamoto M, Itoh K.
    • Journal Title

      J. Biochem. (Tokyo)

      Volume: 150 Pages: 209-217

    • NAID

      10030587033

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 酸化ストレス誘導性遺伝子HO-1発現を制御するnon-codingRNAsの解析2012

    • Author(s)
      丸山敦史、伊東健
    • Organizer
      第85回日本生化学会大会(ポスター発表)
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] Nrf2 induces the Z-DNA formation in the human HO-1 promoter. 7th International Meeting on Heme Oxygenases & Related Enzymes (Poster presentation)2012

    • Author(s)
      Maruyama A, Itoh K
    • Organizer
      University of Edinburgh
    • Place of Presentation
      Edinburgh (英国)
    • Related Report
      2012 Final Research Report
  • [Presentation] Nrf2 induces the Z-DNA formation in the human HO-1 promoter.2012

    • Author(s)
      Maruyama Atsushi
    • Organizer
      7th International Meeting on Heme Oxygenases & Related Enzymes
    • Place of Presentation
      University of Edinburgh, Edinburgh (英国)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 酸化ストレス誘導性遺伝子HO-1発現を制御するnon-coding RNAsの解析2012

    • Author(s)
      丸山敦史
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nrf2 enhances PolII andBRG1 binding to the human HO-1 promoter2011

    • Author(s)
      Maruyama A, Itoh K
    • Organizer
      第34回日本分子生物学会年会(ポスター発表)
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Nrf2なヒトHO-1遺伝子発現活性化機構の解析2011

    • Author(s)
      丸山敦史、伊東健
    • Organizer
      第77回日本生化学会東北支部例会(口頭発表)
    • Place of Presentation
      仙台
    • Year and Date
      2011-07-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Nrf2なヒトHO-1遺伝子発現活性化機構の解析2011

    • Author(s)
      丸山敦史,伊東健
    • Organizer
      第77回日本生化学会 東北支部例会
    • Place of Presentation
      仙台
    • Related Report
      2011 Research-status Report
  • [Presentation] Nrf2 enhances PolII and BRG1 binding to the human HO-1 promoter.2011

    • Author(s)
      Atsushi Maruyama, Ken Itoh
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Research-status Report
  • [Book] 実験医学増刊、活性酸素・ガス状分子による恒常性制御と疾患2012

    • Author(s)
      丸山敦史、伊東健
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 実験医学増刊、活性酸素・ガス状分子による恒常性制御と疾患2012

    • Author(s)
      丸山敦史
    • Total Pages
      8
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Remarks] 弘前大学大学院医学研究科附属高度先進医学研究センター分子生体防御学講座

    • URL

      http://www.med.hirosaki-u.ac.jp~/admed/department/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 弘前大学大学院医学研究科附属高度先進医学研究センター分子生体防御学講座

    • URL

      http://www.med.hirosaki-u.ac.jp/~admed/department/index.html

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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