| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Left-handed double helix of DNA, called Z-DNA, is an alternative structure of DNA and appears to have many diverse roles in nuclear processes such as transcriptional regulation and nucleosome positioning. In this study, I have developed a method for in vivo detection of Z-DNA formation and show Nrf2 activation is associated with Z-DNA formation in the HO-1 gene promoter. The results implicate Nrf2-dependent Z-DNA formation in HO-1 gene transcriptional activation and suggestthe involvement of BRG1 in Z-DNA formation. Human HO-1 gene promoter might be a good model system to understand Z-DNA formation and how Z-DNA forming sequences regulate geneexpression.
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