| Project/Area Number |
23790322
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
MUTO Akihiko 東北大学, 大学院・医学系研究科, 准教授 (80343292)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 遺伝子 / 発現制御 / 転写因子 / Bach2 / ユビキチン / B細胞 |
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| Research Abstract |
In immune response, B cell fate might be regulated by the amount of ubiquitinated Bach2 proteins. However, in vitro recombinant protein assay revealed that MIPP and Klhl9, which are adaptor proteins in ubiquitination system, didn't enhance the ubiquitination of Bach2 protein. Furthermore, by proteomics analysis using MS spectrometry and mutagenesisfor Bach2 protein, phospholylation sites on Bach2 protein were identified.
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