Studies of chromatin architectural protein HMGN3 in pancreaticbeta-cell

• Project/Area Number: 23790323
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Yamagata University
• Principal Investigator: KURAHASHI Toshihiro 山形大学, 大学院・医学系研究科, 助教 (00596570)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: HMGN / クロマチン / 膵β細胞 / 国際情報交流 (米国・NIH)

Research Abstract

We studied physiological roles of high mobility group N (HMGN) and obtained following results. (1) HMGN3 was highly expressed in pancreatic endocrine cells, and -knockout mouse showed a diabetic phenotype. (2) HMGN3 might interact with a splicing factor. (3) Overexpression of HMGN3 affected the gene expression little in fibroblast but largely in pancreatic beta cell-derived cell. (4) Opposite results were obtained by overexpression of HMGN3 in these cells. HMGN1 interacted with PCNA and facilitated its binding to chromatin.

Research Products

• [Journal Article] A malfunction in triglyceride transfer from the intracellular lipid pool to apoB in enterocytes of SOD1-deficient mice (2012)
  • Author(s): Kurahashi T, Konno T, Otsuki N, Kwon M, Tsunoda S, Ito J, Fujii J
  • Journal Title: FEBS Lett Volume: 586 (24) Pages: 4289-95
  • Peer Reviewed
• [Journal Article] The Nucleosome Binding Protein HMGN1 Interacts with PCNA and Facilitates Its Binding to Chromatin. (2012)
  • Author(s): T. Kurahashi
  • Journal Title: Molecular and cellular biology Volume: 32(10) Issue: 10 Pages: 1844-54
  • DOI: 10.1128/mcb.06429-11
  • Peer Reviewed
• [Journal Article] Effects of HMGN variants on the cellular transcription profile (2011)
  • Author(s): Rochman M, Taher L, Kurahashi T, Cherukuri S,Landsman D,Ovcharenko I and Bustin M
  • Journal Title: Nucleic Acid Res Volume: 39(10) Issue: 10 Pages: 4076-87
  • DOI: 10.1093/nar/gkq1343
  • Peer Reviewed
• [Presentation] 酸化ストレス亢進による小腸の脂質代謝異常 (2012)
  • Author(s): 倉橋敏裕ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡、福岡国際会議場・マリンメッセ福岡
• [Presentation] SOD1 deficiency causes postprandial dyslipidemia due to impaired lipid metabolism in the intestine (2012)
  • Author(s): Kurahashi, T, et al
  • Organizer: 16th Biennial Meeting of Society for Free Radical Research International
  • Place of Presentation: Imperial College, London, UK, Sep
• [Presentation] SOD1欠損マウス小腸における脂質代謝の解析 (2012)
  • Author(s): 倉橋敏裕ら
  • Organizer: 第65回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島、徳島郷土文化会館