Induction of multipotent germline stem cells from germline stem cells by epigenetic change.

• Project/Area Number: 23790332
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: TANAKA Takashi 京都大学, 医学研究科, 助教 (40579265)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / エピジェネティクス / ヒストン修飾 / 多能性幹細胞 / Small RNA / 生殖細胞 / 幹細胞

Research Abstract

To el uci date the mechani sm whi ch represses pl uri potency i n germline stem cells (GSCs), this study investigated whether epigenetic changes induce GS-mGS conversion. As a result, lentiviral transduction of shrnas for epigenetic modifiers did not induce GS-mGS conversion. These results suggest that pluripotency in GS cells is repressed by several pathways.

Research Products

• [Journal Article] Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice (2012)
  • Author(s): Masuda T, et al.
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 27 Pages: 10885-108901
  • DOI: 10.1073/pnas.1121524109
  • Peer Reviewed
• [Journal Article] In Vitro Transformation of Mouse Testis Cells by Oncogene Transfection (2012)
  • Author(s): 森本裕子、李知英、田中敬、石井慧、豊國信哉、篠原美都、篠原隆司
  • Journal Title: Biology of Reproduction Volume: 86 Issue: 5 Pages: 1-11
  • DOI: 10.1095/biolreprod.111.095307
  • Peer Reviewed
• [Journal Article] In vitro transformation of mouse testis cells by oncogene transfection. (2012)
  • Author(s): Morimoto H, Lee J, Tanaka T, Ishii K, Toyokuni S, Kanatsu-Shinohara M, Shinohara T.
  • Journal Title: Biology of Reproduction Volume: in publication
  • Peer Reviewed
• [Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier (2011)
  • Author(s): Takashima, S, . Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T
  • Journal Title: Cell Stem Cell Volume: 9 Issue: 5 Pages: 463-475
  • DOI: 10.1016/j.stem.2011.08.011
  • Peer Reviewed
• [Journal Article] Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis (2011)
  • Author(s): Tanaka T, et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108 Issue: 26 Pages: 10579-10584
  • DOI: 10.1073/pnas.1015447108
  • Peer Reviewed
• [Journal Article] Homologous recombination in rat germline stem cells (2011)
  • Author(s): Kanatsu-Shinohara M., et al
  • Journal Title: Biol Reprod Volume: 85 Issue: 1 Pages: 208-17
  • DOI: 10.1095/biolreprod.111.090837
  • Peer Reviewed
• [Journal Article] Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier. (2011)
  • Author(s): Takashima S, Kanatsu-Shinohara M, Tanaka T, Takehashi M, Morimoto H, Shinohara T.
  • Journal Title: Cell Stem Cell Volume: 9 Pages: 463-475
  • Peer Reviewed
• [Presentation] インテグリンシグナルによる精子幹細胞制御 (2011)
  • Organizer: 蛋白研セミナー
  • Place of Presentation: 大阪大学蛋白質研究所(招待講演)蛋白質研究所
  • Year and Date: 2011-12-01
• [Presentation] インテグリンシグナルによる精子幹細胞制御
  • Author(s): 田中 敬
  • Organizer: 蛋白研セミナー(招待講演)
  • Place of Presentation: 大阪大学 蛋白質研究所（大阪府）
• [Remarks]
  • URL: http://www2.mfour.med.kyoto-u.ac.jp/~molgen