Spatial regulation of branching tubulogenesis by Wnt signaling

• Project/Area Number: 23790333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: MATSUMOTO Shinji 大阪大学, 医学系研究科, 助教 (20572324)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 分枝管腔形成 / Wnt シグナル / Wntシグナル / 上皮 / Wnt / 極性

Research Abstract

Novel factor X regulating epithelial branching tubulogenesis induced by Wnt3a and EGF was identified by microarray analysis. Factor X activated Rac and inhibited Rho respectively, resulting in cell extension and ac tivation of cell motility. Cell morphological change induced cell proliferation in three dimensional matrix. These results indicate that Wnt and EGF signaling regulates epithelial branching tubulogenesis cooperatively.

Research Products

• [Journal Article] The Dishevelled-associating protein Daple controls the non-canonical Wnt/Rac pathway and cell motility. (2012)
  • Author(s): Ishida-Takagishi M, Enomoto A, Asai N, Ushida K, Watanabe T, Hashimoto T, Kato T, Weng L, Matsumoto S, Asai M, Murakumo Y, Kaibuchi K, Kikuchi A, Takahashi M.
  • Journal Title: Nat Commun Volume: 3 Pages: 859-859
  • Peer Reviewed
• [Journal Article] Regulation of focal adhesion dynamics by Wnt5a signaling. (2012)
  • Author(s): Matsumoto S, Kikuchi A.
  • Journal Title: Methods Mol Biol Volume: 839 Pages: 215-27
  • Peer Reviewed
• [Journal Article] An anti-Wnt5a antibody suppresses metastasis of gastric cancer cells in vivo by inhibiting receptor-mediated endocytosis (2012)
  • Author(s): Hanaki H, Yamamoto H, Sakane H, Matsumoto S, Ohdan H, Sato A, and Kikuchi A.
  • Journal Title: Mol Cancer Ther Volume: 11 Pages: 298-307
  • Peer Reviewed
• [Journal Article] Localization of glypican-4 in different membrane microdomains is involved in the regulation of Wnt signaling. (2012)
  • Author(s): Sakane H, Yamamoto H, Matsumoto S, Sato A, and Kikuchi A.
  • Journal Title: J Cell Sci Volume: 125 Pages: 449-60
  • Peer Reviewed
• [Journal Article] The Dishevelled-associating protein Daple controls the non-canonical Wnt/Rac pathway and cell motility (2012)
  • Author(s): Ishida-Takagishi M, Enomoto A, et al.
  • Journal Title: Nat Commun Volume: 3 Issue: 1 Pages: 859-859
  • DOI: 10.1038/ncomms1861
  • Peer Reviewed
• [Journal Article] Localization of glypican-4 in different membrane microdomains is involved in the regulation of Wnt signaling (2012)
  • Author(s): Sakane, H., et.al.
  • Journal Title: J.Cell Sci. Volume: 125 Issue: 2 Pages: 449-460
  • DOI: 10.1242/jcs.091876
  • Peer Reviewed
• [Journal Article] An Wnt5a-antibody suppresses metastasis of gastric cancer cells in vivo by inhibiting receptor-mediated endocytosis (2012)
  • Author(s): Hanaki, H., et.al.
  • Journal Title: Mol.Cancer Ther. Volume: 11 Issue: 2 Pages: 298-307
  • DOI: 10.1158/1535-7163.mct-11-0682
  • Peer Reviewed
• [Journal Article] Regulation of focal adhesion dynamics by Wnt5a signaling (2012)
  • Author(s): Matsumoto, S., et.al.
  • Journal Title: Methods Mol.Biol. Volume: 839 Pages: 215-27
  • DOI: 10.1007/978-1-61779-510-7_17
  • ISBN: 9781617795091, 9781617795107
  • Peer Reviewed
• [Journal Article] New insights into the mechanism of Wnt signaling pathway activation (2011)
  • Author(s): Kikuchi, A
  • Journal Title: Int Rev Cell Mol Biol. Volume: 291 Pages: 21-71
  • DOI: 10.1016/b978-0-12-386035-4.00002-1
  • ISBN: 9780123860354
  • Peer Reviewed
• [Journal Article] New insights into the mechanism of Wnt signaling pathway activation.
  • Author(s): Kikuchi A, Yamamoto H, Sato A, Mastumoto S.
  • Journal Title: Int Rev Cell Mol Biol Volume: 291 Pages: 21-71
  • Peer Reviewed
• [Presentation] Wnt/β-catenin シグナルとEGF シグナルの協調による三次元基質内での上皮分枝管腔形成制御機構の解析 (2012)
  • Author(s): 松本 真司
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Wnt/β-cateninシグナルとEGFシグナルの協調による三次元基質内での上皮分枝管腔形成制御機構の解析 (2012)
  • Author(s): 松本 真司
  • Organizer: 第３５回分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
• [Presentation] Wnt3a and Wnt5a regulate epithelial polarity and tubulogenesis cooperatively (2011)
  • Author(s): 松本 真司
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Wnt3a and Wnt5a regulate epithelial polarity and tubulogenesis cooperatively (2011)
  • Author(s): 松本真司
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Book] 細胞工学 Vol.32 No.4 (2013)
  • Author(s): 松本 真司
  • Total Pages: 496
  • Publisher: 秀潤社
• [Book] 実験医学増刊 Vol.30 No.5 (2012)
  • Author(s): 菊池 章、松本真司
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] がん生物学イラストレイテッド (2011)
  • Author(s): 菊池 章、松本真司
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 実験医学増刊 Vol.29 No.2 (2011)
  • Author(s): 菊池 章、佐藤 朗、松本 真司
  • Total Pages: 10
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/