Function of a novel ER-localized transmembrane lipase-like protein in epidermal barrier formation

• Project/Area Number: 23790334
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Osaka University
• Principal Investigator: OHGAKI Ryuichi 大阪大学, 大学院・医学系研究科, 助教 (20467525)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: リパーゼ / 表皮バリア / 角質細胞間脂質 / 脂質代謝異常 / 生体分子医学 / 組織形成 / 脂質代謝

Research Abstract

Epidermal barrier is crucial for survival of organisms. Homozygous gene disruption of a lipase-like protein LOG1 in mouse is associated with an abnormal water loss from skin and results in lethality at one day post-partum although the detailed mechanism is unknown. This study revealed an augmentation in water permeability and an abnormal lipid composition of stratum corneum in gene knockout mice. It was suggested that LOG1 is a novel transmembrane-type lipase which is involved in the metabolism of intercellular lipids of stratum corneum and is fundamentally important for epidermal barrier.

Research Products

• [Journal Article] NRFL-1, the C. elegans NHERF orthologue, interacts with amino acid transporter 6 (AAT-6) for age-dependent maintenance of AAT-6 on the membrane (2012)
  • Author(s): Hagiwara K, Nagamori S, Umemura YM, Ohgaki R, Tanaka H, Murata D, Nakagomi S, Nomura KH, Kage-Nakadai E, Mitani S, Nomura K, Kanai Y
  • Journal Title: PLoS One Volume: 7
• [Journal Article] Establishment of stable cell lines with high expression of heterodimers of human 4F2hc and human amino acid transporter LAT1 or LAT2 and delineation of their differential interaction with α-alkyl moieties (2012)
  • Author(s): Khunweeraphong N, Nagamori S, Wiriyasermkul P, Nishinaka Y, Wongthai P, Ohgaki R, Tanaka H,Tominaga H, Sakurai H, Kanai Y
  • Journal Title: J Pharmacol Sci Volume: 119 Pages: 368-380
  • NAID: 10031071638
• [Journal Article] Transport of 3-fluoro-L-alpha-methyl-tyrosine by tumor-upregulated L-type amino acid transporter 1: a cause of the tumor uptake in PET (2012)
  • Author(s): Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y
  • Journal Title: J Nucl Med Volume: 53 Pages: 1253-1261
• [Journal Article] Linkage of N-cadherin to multiple cytoskeletal elements revealed by a proteomic approach in hippocampal neurons (2012)
  • Author(s): Tanaka H, Takafuji K, Taguchi A, Wiriyasermkul P, Ohgaki R, Nagamori S, Suh PG, Kanai Y.
  • Journal Title: Neurochem Int Volume: 61 Pages: 240-250
• [Journal Article] NRFL-1, the C. elegans NHERF Orthologue, Interacts with Amino Acid Transporter 6 (AAT-6) for Age-Dependent Maintenance of AAT-6 on the Membrane. (2012)
  • Author(s): Kohei Hagiwara
  • Journal Title: Plos one Volume: 7(8) Issue: 8 Pages: e43050-e43050
  • DOI: 10.1371/journal.pone.0043050
  • Peer Reviewed
• [Journal Article] Establishment of stable cell lines with high expression of heterodimers of human 4F2hc and human amino acid transporter LAT1 or LAT2 and delineation of their differential interaction with α-alkyl moieties. (2012)
  • Author(s): Khunweeraphong N, Nagamori S, Wiriyasermkul P, Nishinaka Y, Wongthai P, Ohgaki R, Tanaka H, Tominaga H, Sakurai H, Kanai Y.
  • Journal Title: Journal of Pharmacological Science Volume: 119 Pages: 368-380
  • NAID: 10031071638
  • Peer Reviewed
• [Journal Article] Transport of 3-fluoro-L-α-methyl-tyrosine by tumor-upregulated L-type amino acid transporter 1: a cause of the tumor uptake in PET. (2012)
  • Author(s): Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y.
  • Journal Title: J Nucl Med. Volume: 53 Issue: 8 Pages: 1253-1261
  • DOI: 10.2967/jnumed.112.103069
  • Peer Reviewed
• [Journal Article] Linkage of N-cadherin to multiple cytoskeletal elements revealed by a proteomic approach in hippocampal neurons. (2012)
  • Author(s): Tanaka H, Takafuji K, Taguchi A, Wiriyasermkul P, Ohgaki R, Nagamori S, Suh PG, Kanai Y.
  • Journal Title: Neurochem Int. Volume: 61 Issue: 2 Pages: 240-250
  • DOI: 10.1016/j.neuint.2012.05.008
  • Peer Reviewed
• [Journal Article] Transport of 3-fluoro-L-alpha-methyl tyrosine by tumor-upregulated amino acid transporter LAT1: a cause of the tumor uptake in PET. (2012)
  • Author(s): Wiriyasermkul P, Nagamori S, Tominaga H, Oriuchi N, Kaira K, Nakao H, Kitashoji T, Ohgaki R, Tanaka H, Endou H, Endo K, Sakurai H, Kanai Y.
  • Journal Title: Journal of Nuclear Medicine Volume: in press
  • Peer Reviewed
• [Journal Article] Differential effects of TNF (TNFSF2) and IFN-γ on intestinal epithelial cell morphogenesis and barrier function in three-dimensional culture (2011)
  • Author(s): Juuti-Uusitalo K, Klunder LJ, Sjollema KA, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van Ijzendoorn SC
  • Journal Title: PLoS One Volume: 6
• [Journal Article] Organellar Na+/H+ exchangers: novel players in organelle pH regulation and their emerging functions. (2011)
  • Author(s): Ohgaki R, van IJzendoorn SC, Matsushita M, Hoekstra D, Kanazawa H
  • Journal Title: Biochemistry Volume: 50 Pages: 443-450
• [Journal Article] Differential effects of TNF (TNFSF2) and IFN-γ on intestinal epithelial cell morphogenesis and barrier function in three-dimensional culture. (2011)
  • Author(s): Juuti-Uusitalo K, Klunder LJ, Sjollema KA, Mackovicova K, Ohgaki R, Hoekstra D, Dekker J, van Ijzendoorn SC.
  • Journal Title: PLoS One Volume: 6 Issue: 8 Pages: e22967-e22967
  • DOI: 10.1371/journal.pone.0022967
  • Peer Reviewed
• [Presentation] 質量分析計と再構成プロテオリポソームを用いた新規トランスポーター複合体の解析-腎尿細管に残された第二のシスチントランスポーターの分子同定 (2012)
  • Author(s): 永森收志, 奥山裕久, Pattama,Wiriyasermkul, 中込咲綾, 西中由美子, 高藤和輝, 大垣隆一, 金井好克
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡、日本
  • Year and Date: 2012-12-15
• [Presentation] 腎近位尿細管の第二のシスチントランスポーターの分子同定と機能解析 (2012)
  • Author(s): 永森收志, 奥山裕久, Pattama Wiriyasermkul, 中込咲綾, 西中由美子, 高藤和輝, 大垣隆一, 金井好克
  • Organizer: 2012年度 生理研研究会
  • Place of Presentation: 愛知、日本
  • Year and Date: 2012-12-01
• [Presentation] ニコチンによる海馬神経細胞スパインの形態変化(3) (2012)
  • Author(s): 田中秀和、Pattama Wiriyasermkul、忠垣憲次郎、大垣隆一、永森收志、金井好克
  • Organizer: 第122回日本薬理学会近畿部会
  • Place of Presentation: 豊中市
  • Year and Date: 2012-11-16
• [Presentation] 腎尿細管に残された第二のシスチントランスポーターの分子同定 (2012)
  • Author(s): 大垣隆一, 奥山裕久, Pattama Wiriyasermkul, 中込咲綾, 西中由美子, 高藤和輝, 永森收志, 金井好克.
  • Organizer: 第3回分子腎臓フォーラム
  • Place of Presentation: 東京、日本
  • Year and Date: 2012-09-01
• [Presentation] 特異プロスタグランジン輸送体OAT -PGノックアウトマウスの食塩応答性血圧変動の機序の解明 (2012)
  • Author(s): 金井好克, 永森收志, 大垣隆一, 中込咲綾, Wiriyasermkul Pattama, 西山俊.腎
  • Organizer: ソルト・サイエンス研究財団「第24回助成研究発表会」
  • Place of Presentation: 東京
  • Year and Date: 2012-07-18
• [Presentation] 表皮バリア形成に必須の新規リパーゼ様タンパク質mYEH2の役割. 花王芸術科学財団 (2012)
  • Author(s): 大垣隆一
  • Organizer: 第14回 助成研究発表・交流会
  • Place of Presentation: 東京、日本
  • Year and Date: 2012-06-06
• [Presentation] Functional coupling between a Na+-dependent anion transporter SMCT2 and a urate-anion exchanger URAT1 through a scaffold protein PDZK1 (2012)
  • Author(s): Nagamori S, Umemura Y, Wiriyasermkul P, Nakagomi S, Nishinaka Y, Takafuji K, Ohgaki R, Kanai Y
  • Organizer: Gordon Research Conferences Membrane Transport Proteins
  • Place of Presentation: Les Diablerets, Switzerland.
• [Presentation] Functional coupling between a Na+-dependent anion transporter SMCT2 and a urate-anion exchanger URAT1 through a scaffold protein PDZK1. (2012)
  • Author(s): Nagamori S, Umemura Y, Wiriyasermkul P, Nakagomi S, Nishinaka Y, Takafuji K, Ohgaki R, Kanai Y.
  • Organizer: Gordon Research Conferences, Membrane Transport Proteins.
  • Place of Presentation: Les Diablerets, Switzerland
• [Presentation] System L amino acid transporter LAT1 is essential for embryonic angiogenesis (2011)
  • Author(s): 大垣隆一, Li Yuewei, 永森收志, 田中秀和, 金井好克
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 日本、京都.
  • Year and Date: 2011-09-22
• [Presentation] System L amino acid transporter LAT1 is essential for embryonic angiogenesis (2011)
  • Author(s): 大垣隆一、Li Yuewei、永森收志、田中秀和、金井好克
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都国際会館
• [Presentation] 表皮バリア形成に必須の 新規リパーゼ様タンパク質mYEH2の役割
  • Author(s): 大垣隆一
  • Organizer: 花王芸術科学財団 第14回 助成研究発表・交流会
  • Place of Presentation: 東京、日本
• [Presentation] 腎尿細管に残された第二のシスチントランスポーターの分子同定
  • Author(s): 大垣 隆一, 奥山 裕久, Pattama Wiriyasermkul, 中込 咲綾, 西中 由美子, 高藤 和輝, 永森 收志, 金井 好克
  • Organizer: 第3回分子腎臓フォーラム
  • Place of Presentation: 東京、日本
• [Presentation] 腎近位尿細管の第二のシスチントランスポーターの分子同定と機能解析
  • Author(s): 永森收志、奥山 裕久、Pattama Wiriyasermkul、中込 咲綾、西中 由美子、高藤 和輝、大垣 隆一、金井 好克
  • Organizer: 2012年度 生理研研究会
  • Place of Presentation: 愛知、日本
• [Presentation] 質量分析計と再構成プロテオリポソームを用いた新規トランスポーター複合体の解析－腎尿細管に残された第二のシスチントランスポーターの分子同定
  • Author(s): 永森收志、奥山 裕久、Pattama Wiriyasermkul、中込 咲綾、西中 由美子、高藤 和輝、大垣 隆一、金井 好克
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡、日本