Roles of protein tyrosine phosphatase SAP-1 in the intestinal immunity.

• Project/Area Number: 23790353
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kobe University (2012); Gunma University (2011)
• Principal Investigator: KOTANI Takenori 神戸大学, 医学研究科, 助教 (40455960)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 腸管免疫 / チロシンホスファターゼ / チロシンリン酸化 / 腸炎 / 腸粘膜上皮細胞 / チロシンキナーゼ

Research Abstract

SAP-1 is a receptor-type protein tyrosine phosphatase that specifically localizes to microvilli of the brush border in gastrointestinal epithelial cells. In this study, I found that SAP-1 and p100 interact through their ectodomains and form a complex. I also found that SAP-1/p100 regulates the production of chemokine and the phagocytosis of HEK293 cells, suggesting intestinal immunity might be regulated by these physiological functions of SAP-1/p100.

Research Products

• [Journal Article] Dendritic cell-specific ablation of the protein tyrosine phosphatase shp1 promotes Th1 cell differentiation and induces autoimmunity. (2012)
  • Author(s): Kaneko T., Saito Y., Kotani T., Okazawa H., Iwamura H., Sato-Hashimoto M., Kanazawa Y., Takahashi S., Hiromura K., Kusakari S.Kaneko Y., Murata Y., Ohnishi H., Nojima Y., Takagishi K., and Matozaki T.
  • Journal Title: J. Immunol Volume: 188 Issue: 11 Pages: 5397-5407
  • DOI: 10.4049/jimmunol.1103210
  • Peer Reviewed
• [Journal Article] Hypothermia-dependent and -independent effects of forced swim on the phosphorylation states of signaling molecules in mouse hippocampus. (2012)
  • Author(s): Hayashi Y
  • Journal Title: Biochemical and biophysical research communications. Volume: 428 Issue: 4 Pages: 475-481
  • DOI: 10.1016/j.bbrc.2012.10.083
  • Peer Reviewed
• [Journal Article] Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain (2012)
  • Author(s): Maruyama T., Kusakari S., Sato-Hashimoto M., Hayashi Y., Kotani T., Murata Y., Okazawa H., Oldenborg P.-A., Kishi S., Matozaki T., and Ohnishi H.
  • Journal Title: J. Neurochem Volume: 121 Issue: 6 Pages: 891-902
  • DOI: 10.1111/j.1471-4159.2012.07748.x
  • Peer Reviewed
• [Journal Article] Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity. (2012)
  • Author(s): Kaneko T, Saito Y, Kotani T, Okazawa H, Iwamura H, Sato-Hashimoto M, Kanazawa Y, Takahashi S, Hiromura K, Kusakari S, Kaneko Y, Murata Y, Ohnishi H, Nojima Y, Takagishi K, Matozaki T.
  • Journal Title: The Journal of Immunology Volume: in press
  • Peer Reviewed
• [Journal Article] Signal regulatory protein α regulates the homeostasis of T lymphocytes in the spleen. (2011)
  • Author(s): Sato-Hashimoto M., Saito Y., Ohnishi H., Iwamura H., Kanazawa Y., Kusakari S., Kotani T., Mori M., Murata Y., Okazawa H., Ware CF., Olodenbrg PA., Nojima Y., and Matozaki T.
  • Journal Title: J. Immunol Volume: 187 Issue: 1 Pages: 291-297
  • DOI: 10.4049/jimmunol.1100528
  • Peer Reviewed
• [Presentation] Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiations and induces autoimmunity (2013)
  • Author(s): 小谷 武徳
  • Organizer: 第10回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 東京
• [Presentation] Regulation of intestinal immunity by the protein tyrosine phosphatase SAP-1 (2013)
  • Author(s): 村田 陽二
  • Organizer: 第10回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 東京
• [Presentation] Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain (2013)
  • Author(s): 草苅 伸也
  • Organizer: 第10回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 東京
• [Presentation] Tyrosine phosphorylation of CEACAM20 and its functional roles (2013)
  • Author(s): Edwin Widyanto Daniwijaya
  • Organizer: 第10回プロテインホスファターゼ国際
  • Place of Presentation: 東京
• [Presentation] Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell diffierentiation and induces autoimmunity (2013)
  • Author(s): Kotani Takenori
  • Organizer: 第10回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 東京
• [Presentation] Tyrosine phosphorylation of CEACAM20 and its functional roles (2013)
  • Author(s): Edwin Widyanto Daniwijaya
  • Organizer: 第10回プロテインホスファターゼ国際カンファレンス
  • Place of Presentation: 東京
• [Presentation] 成熟脳における Shp2の生理機能解析 (2012)
  • Author(s): 草苅伸也
  • Organizer: 第5回プロテインホスファターゼ研究会 学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-01-19
• [Presentation] 腸微絨毛特異的な発現を示す受容体型チロシンホスファターゼSAP-1 による腸管免疫制御 (2012)
  • Author(s): 小谷武徳
  • Organizer: 第5回プロテインホスファターゼ研究会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-01-19
• [Presentation] 脾臓T細胞の恒常性調節へのSIRPαの関与 (2012)
  • Author(s): 橋本(佐藤) 美穂
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 細胞質型チロシンホスファターゼShp2の成熟脳における機能解析 (2012)
  • Author(s): 草苅伸也
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] Understanding of synapse pathologyl, from genome mutation to functional defects (2012)
  • Author(s): 草苅伸也
  • Organizer: Regulation of brain functions by a protein tyrosine phosphatase Shp2 International Synapse Research Workshop 2012
  • Place of Presentation: 岡崎
• [Presentation] Roles of vascular endothelial protein tyrosine phosphatase (VE-PTP) in endothelial cell (2012)
  • Author(s): Kemala Isnainiasih Mantilidewi
  • Organizer: 2nd Bandung Biomolecular Medicine Conference
  • Place of Presentation: インドネシア・バンドン
• [Presentation] Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain (2012)
  • Author(s): 草苅伸也
  • Organizer: 11th biennial meeting of APSN/55th Meeting of JSN
  • Place of Presentation: 神戸
• [Presentation] 細胞質型チロシンホスファターゼShp2の成熟脳における機能解析 (2012)
  • Author(s): 草苅伸也
  • Organizer: 第11回生体機能研究会
  • Place of Presentation: 箱
• [Presentation] IMPORTANCE OF THE PROTEIN TYROSINE PHOSPHATASE SHP1 IN DENDRITIC CELLS FOR PREVENTION OF TH1 CELL DIFFERENTIATION AND AUTOIMMUNITY: A POTENTIAL TARGET FOR THE THERAPY (2012)
  • Author(s): 金子哲也
  • Organizer: European League Against Rheumatism 2012
  • Place of Presentation: ドイツ・ベルリン
• [Presentation] 細胞質型チロシンホスファターゼShp2の成熟脳における機能解析 (2012)
  • Author(s): 草苅伸也
  • Organizer: 第11回生体機能研究会
  • Place of Presentation: 箱根
• [Presentation] Roles of vascular endothelial protein tyrosine phosphatase (VE-PTP) in endothelial cells (2012)
  • Author(s): Kemala Isnainiasih Mantilidewi
  • Organizer: 2nd Bandung Biomolecular Medicine Conference
  • Place of Presentation: インドネシア・バンドン
• [Presentation] Regulation of brain functions by a protein tyrosine phosphatase Shp2 (2012)
  • Author(s): Kusakari Shinya
  • Organizer: International Synapse Research Workshop 2012 "Understanding of synapse pathologyl, from genome mutation to functional defects"
  • Place of Presentation: 岡崎
• [Presentation] 腸微絨毛特異的な発現を示す受容体型チロシンホスファターゼSAP-1による腸管免疫制御 (2012)
  • Author(s): 小谷武徳、村田陽二、Yana Supriatna、岡澤秀樹、大西浩史、Edwin Daniwijaya、草苅伸也、定方久延、大川克也、的崎尚
  • Organizer: 第5回プロテインホスファターゼ研究会 学術集会 大阪
  • Place of Presentation: 大阪大学 吹田キャンパス（銀杏会館）,大阪
• [Presentation] 成熟脳におけるShp２の生理機能解析 (2012)
  • Author(s): 草苅伸也、小谷武徳、橋本美穂、村田陽二、岡澤秀樹、吾郷由紀夫、松田敏夫、Benjamin G. Neel、的崎尚、大西浩史
  • Organizer: 第5回プロテインホスファターゼ研究会 学術集会 大阪
  • Place of Presentation: 大阪大学 吹田キャンパス（銀杏会館）,大阪
• [Presentation] Dendritic cell-specific depletion of protein-tyrosine phosphatase Shp1 promotes Th1 differentiation and autoimmunity (2011)
  • Author(s): 金子哲也
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 2011-11-29
• [Presentation] Dendritic cell-specific depletion of protein-tyrosine phosphatase Shp1 promotes Th1 differentiation and autoimmunity (2011)
  • Author(s): KANEKO Tetsuya, SAITO Yasuyuki, OHNISHI Hiroshi, SATO-HASHIMOTO Miho, KOTANI Takenori, MURATA Yoji, OKAZAWA Hideki, TAKAGISHI Kenji, MATOZAKI Takashi
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ,千葉
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/tougou/signal/Home.html
• [Remarks]
  • URL: http://www.med.kobe-u.ac.jp/tougou/signal/index.html