Molecular basis of Rab27 GTPase-mediated regulated exocytosis

• Project/Area Number: 23790354
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Gunma University
• Principal Investigator: MATSUNAGA Kohichi 群馬大学, 生体調節研究所, 助教 (20570162)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: β細胞 / Rab27 / 調節性分泌 / インスリン顆粒 / 国際情報交流

Research Abstract

To investigate the molecular mechanism of Rab27 GTPase-mediated regulated exocytosis, I performed comprehensive proteomic analysis of Rab27a binding proteins in various secreting cells. I identified dozens of novel Rab27a binding proteins and also identified cell strain specific binding proteins. Therefore, I obtained basic data to clarify the molecular mechanism for regulating Rab27-mediated exocytosis pathway.