Investigation of apoC-III on atherosclerosis
| Project/Area Number |
23790359
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kobe University (2012)
University of Yamanashi (2011) |
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Principal Investigator |
KOIKE Tomonari 神戸大学, 大学院・医学研究, 助教 (40432158)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 分子病態学 / 動脈硬化 / アポリポ蛋白CIII / トランスジェニックウサギ / ウサギ |
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| Research Abstract |
Features of lipoprotein metabolism in rabbits, unlike mouse, resemble to human, we planned to make human apoCIII transgenic (Tg) rabbits to reveal its functions on lipoprotein metabolism and atherogenesis. For the first year, we injected more than 500 embryos but failed to obtain any positiveTg rabbits due to low number of living pups born. After that, we modified the protocol of microinjection. In the second year, we successfully got 48 alive pups. Although we still did not get any positive rabbits that carry human apoCIII gene, it is quite possible that we will be successful soon. The work is under way for the further experiment.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Human apolipoprotein A-II protects against diet-induced atherosclerosis in transgenic rabbits2013
Author(s)
Wang Y, Niimi M, Nishijima K, Waqar AB, Yu Y, Koike T, Kitajima S, Liu E, Inoue T, Kohashi M, Keyamura Y, Yoshikawa T, Zhang J, Ma L, Zha X, Watanabe T, Asada Y, Chen YE, Fan J
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Journal Title
Arterioscler Thromb Vasc Biol.
Volume: 33
Pages: 224-231
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