| Project/Area Number |
23790360
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脊髄損傷 / 神経可塑性 / プロテオグリカン / 神経糖鎖生物学 |
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| Research Abstract |
We have revealed that keratan sulfate (KS), which is a kind ofglycosaminoglycan, strongly inhibits the neurite outgrowth as well as chondroitin sulfate (CS). In this study, I aimed the identification of KS/CSPG and the evaluation of the mechanism recognizing KS/CSPG by neurons. KS/CSPGs were isolated from the injured spinal cords and reactive-astrocytes stimulated by the growth factors using column chromatography, and were identified by the shot-gun proteomics method. Phosphacan was mainly identified as the inhibitory KS/CSPG. Interestingly, the inhibitory KS/CSPG isolated from postnatal 1 day rat brain was also identified as phosphacan. Therefore, I suggest that the injured neurons and developing neurons recognize KS/CSPG in the common manner. In addition, the recombinant KS-bearing phaophacan strongly inhibited the neurite outgrowth, and the deletion of KS binding domain of phosphacan ameliorated the neurite outgrowth. From these result, I suggest that neurons strongly recognize keratan sulfate on phosphacan and inhibit neurite outgrowth.
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