Analysis of CD44 role in metabolism of leukemic stem cell

• Project/Area Number: 23790376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Keio University
• Principal Investigator: SUGIHARA Eiji 慶應義塾大学, 医学部, 特任助教 (50464996)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 白血病幹細胞 / CD44 / 酸化ストレス / 白血病 / 癌 / 代謝 / 癌幹細胞 / シグナル伝達

Research Abstract

Leukemic stem cells (LSCs) have been reported to be implicated in the maintenance of overall tumor and tumor recurrence. However the mechanisms of these events are largely unknown. In this study, we established B-ALL and CML mice models derived from bone

Research Products

• [Journal Article] Arf/p53 module, which induces apoptosis, downregulates histone H2AX to allow normal cells to survive in the presence of anti-cancer drugs. (2013)
  • Author(s): Atsumi Y, Inase A, Osawa T, Sugihara E, Sakasai R, Fujimori H, Teraoka H, Saya H, Kanno M, Tashiro F, Nakagama H, Masutani M, Yoshioka KI
  • Journal Title: J Biol Chem Volume: (in press)
  • Peer Reviewed
• [Journal Article] Twist2 functions as a tumor suppressor in murine osteosarcoma cells. (2013)
  • Author(s): Ishikawa T, Shimizu T, Ueki A, Yamaguchi S, Onishi N, Sugihara E, Kuninaka S, Miyamoto T, Morioka H, Nakayama R, Kobayashi E, Toyama Y, Mabuchi Y, Matsuzaki Y, Yamaguchi R, Miyano S and Saya H
  • Journal Title: Cancer Sci Volume: (in press) Issue: 7 Pages: 880-888
  • DOI: 10.1111/cas.12163
  • Peer Reviewed
• [Journal Article] Arf and p53 act as guardians of a quiescent cellular state by protecting against immortalization of cells with stable genomes. (2013)
  • Author(s): Osawa T, Atsumi Y, Sugihara E, Saya H, Kanno M, Tashiro F, Masutani M, Yoshioka K.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 432 Issue: 1 Pages: 34-39
  • DOI: 10.1016/j.bbrc.2013.01.091
  • Peer Reviewed
• [Journal Article] IGF1 receptor signaling regulates adaptive radioprotection in glioma stemcells (2013)
  • Author(s): Osuka S. Sampetrean O, Shimizu T, Saga l, Onishi N, Sugihara E, Okubo J, Fujita S, Takano S, Matsumura A, Saya H
  • Journal Title: Stem Cells Volume: 31 Issue: 4 Pages: 627-640
  • DOI: 10.1002/stem.1328
  • Peer Reviewed
• [Journal Article] xCT Inhibition Depletes CD44v-Expressing Tumor Cells That Are Resistant to EGFR-Targeted Therapy in Head and Neck Squamous Cell Carcinoma (2013)
  • Author(s): Yoshikawa M, Tsuchihashi K, Ishimoto T, Yae T, Motohara T, Sugihara E, Onishi N, Masuko T, Yoshizawa K, Kawashiri S, Mukai M, Asoda S, Kawana H, Nakagawa T, Saya H, Nagano O
  • Journal Title: Cancer Res Volume: 73(6) Issue: 6 Pages: 1855-1866
  • DOI: 10.1158/0008-5472.can-12-3609-t
  • Peer Reviewed
• [Journal Article] Complexity of cancer stem cells. (2013)
  • Author(s): Sugihara E, Saya H.
  • Journal Title: Int J Cancer. Volume: 132(6): Issue: 6 Pages: 1249-1259
  • DOI: 10.1002/ijc.27961
  • Peer Reviewed
• [Journal Article] Up-regulation of imp3 confers in vivo tumorigenicity on murine osteosarcoma cells. (2012)
  • Author(s): Ueki A, Shimizu T, Masuda K, Yamaguchi SI, Ishikawa T, Sugihara E, Onishi N, Kuninaka S, Miyoshi K, Muto A, Toyama Y, Banno K, Aoki D, Saya H.
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: e50621-e50621
  • DOI: 10.1371/journal.pone.0050621
  • Peer Reviewed
• [Journal Article] Ink4a and Arf are crucial factors in the determination of the cell of origin and the therapeutic sensitivity of Myc-induced mouse lymphoid tumor (2012)
  • Author(s): Sugihara E, Saya H, et al
  • Journal Title: Oncogene Volume: (印刷中) Issue: 23 Pages: 2849-2861
  • DOI: 10.1038/onc.2011.462
  • Peer Reviewed
• [Journal Article] Loss of p16 expression is associated with the stem cell characteristics of surface markers and therapeutic resistance in estrogen receptor-negative breast cancer (2012)
  • Author(s): Arima Y, Saya H, et al
  • Journal Title: InternationalJournal of Cancer Volume: 130 Issue: 11 Pages: 2568-2579
  • DOI: 10.1002/ijc.26271
  • Peer Reviewed
• [Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells (2012)
  • Author(s): Mayumi Tamada, et al
  • Journal Title: Cancer Research Volume: 72 Issue: 6 Pages: 1438-1448
  • DOI: 10.1158/0008-5472.can-11-3024
  • Peer Reviewed
• [Journal Article] Fibroblast Growth Factor-2 Is an Important Factor that Maintains Cellular Immaturity and Contributes to Aggressiveness of Osteosarcoma (2012)
  • Author(s): Shimizu T., et al
  • Journal Title: Mol Cancer Res Volume: 10(3) Issue: 3 Pages: 454-468
  • DOI: 10.1158/1541-7786.mcr-11-0347
  • Peer Reviewed
• [Journal Article] 癌幹細胞研究の現状と将来 (2012)
  • Author(s): 杉原英志, 佐谷秀行
  • Journal Title: 血液内科 Volume: 64 Pages: 120-126
• [Journal Article] Fibroblast growth factor-2 (Fgf2) is an important factor that maintains cellular immaturity and contributes to aggressiveness of osteosarcoma. (2012)
  • Author(s): Shimizu T, Ishikawa T, Iwai S, Ueki A, Sugihara E, Onishi N, Kuninaka S, Miyamoto T, Toyama Y, Ijiri H, Mori H, Matsuzaki Y, Yaguchi T, Nishio H, Kawakami Y, Ikeda Y and Saya H
  • Journal Title: Mol Cancer Res Volume: 10(3) Pages: 454-468
  • Peer Reviewed
• [Journal Article] Modulation of glucose metabolism by CD44 contributes to antioxidant status and drug resistance in cancer cells. (2012)
  • Author(s): Tamada M, Nagano O, Tateyama S, Ohmura M, Yae T, Ishimoto T, Sugihara E, Onishi N, Yamamoto T, Yanagawa H, Suematsu M and Saya H
  • Journal Title: Cancer Res Volume: 72(6) Pages: 1438-1448
  • Peer Reviewed
• [Journal Article] 幹細胞研究の現状と将来 (2012)
  • Author(s): 杉原英志、佐谷秀行
  • Journal Title: 血液内科 Volume: 64(1) Pages: 120-126
• [Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells (2011)
  • Author(s): Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka A, Akahata M, Kai K, Sugimoto H, Hirao A, and Saya H
  • Journal Title: Neoplasia Volume: 13(9) Pages: 784-791
  • Peer Reviewed
• [Journal Article] Establishment of a Choriocarcinoma Model from Immortalized Normal Extravillous Trophoblast Cells Transduced with HRASV12 (2011)
  • Author(s): Kobayashi Y, Shimizu T, Naoe H, Ueki A, Ishizawa J, Chiyoda T, Onishi N, Sugihara E, Nagano O, Banno K, Kuninaka S, Aoki D, Saya H
  • Journal Title: Am J Pathol. Volume: 179(3) Issue: 3 Pages: 1471-82
  • DOI: 10.1016/j.ajpath.2011.05.019
  • Peer Reviewed
• [Journal Article] The cell cycle regulator Cdh1 controls the pool sizes of hematopoietic stem cells and mature lineage progenitors by protecting from genotoxic stress (2011)
  • Author(s): Ishizawa, J.
  • Journal Title: Cancer Sci Volume: 102 Issue: 5 Pages: 967-974
  • DOI: 10.1111/j.1349-7006.2011.01884.x
  • Peer Reviewed
• [Journal Article] Establishment of a Choriocarcinoma Model from Immortalized Normal Extravillous Trophoblast Cells Transduced with HRASV12 (2011)
  • Author(s): Kobayashi Y, Shimizu T, Naoe H, Ueki A, Ishizawa J, Chiyoda T, Onishi N, Sugihara E, Nagano O, Banno K, Kuninaka S, Aoki D, Saya H
  • Journal Title: Am J Pathol Volume: 179(3) Pages: 1471-1482
  • Peer Reviewed
• [Journal Article] Invasion precedes tumor mass formation in a malignant brain tumor model of genetically modified neural stem cells. (2011)
  • Author(s): Sampetrean O, Saga I, Nakanishi M, Sugihara E, Fukaya R, Onishi N, Osuka A, Akahata M, Kai K, Sugimoto H, Hirao A, and Saya H
  • Journal Title: Neoplasia Volume: 13(9) Pages: 784-791
  • Peer Reviewed
• [Presentation] Tumor cells derived from different cells of origin show distinct therapeutic sensitivities in Mycinducedlymphoid tumor model (2012)
  • Author(s): Sugihara E, Saya H
  • Organizer: Annual meeting of American Society for Cell Biology (ASCB)
  • Place of Presentation: San Francisco, USA
• [Presentation] Identification and characterization of cancer stem like chemotherapeutic resistant cells in mouse pre-B ALL model (2012)
  • Author(s): Sugihara E, Hashimoto N, Saya H
  • Organizer: 第71回日本癌学会総会
  • Place of Presentation: 札幌
• [Presentation] Identification and characterization of cancer stem like chemotherapeutic resistant cells in mouse pre-B ALL model (2012)
  • Author(s): Sugihara E, Saya H
  • Organizer: 日本癌学会
  • Place of Presentation: 札幌
• [Presentation] umor cells derived from different cells of origin show distinct therapeutic sensitivities in Myc-induced lymphoid tumor model (2012)
  • Author(s): Sugihara E, Saya H.
  • Organizer: American Society of Cell Biology
  • Place of Presentation: 米国サンフランシスコ
• [Presentation] Arf and Ink4a are critical factors detemining the cell of origin and therapeutic of Myc-induced mouse lymphoid tumor (2011)
  • Author(s): Sugihara E, Kojima K, Ishizawa J, Micheal A, Saya H
  • Organizer: The 53rd annual meeting of American Society of Hematology (ASH)
  • Place of Presentation: San Diego, USA
• [Presentation] Ink4a and Arf determine the cell of origin and therapeutic sensitivity of Myc-induced lymphoid tumor (2011)
  • Author(s): Sugihara E、Shimizu T, Ishizawa J, Saya H
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] Ink4a and Arf determine the cell of origin and therapeutic sensitivity of Myc-induced lymphoid tumor (2011)
  • Author(s): Sugihara E, Shimizu T, Ishizawa J, Saya H
  • Organizer: 第67回日本血液学会学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Arf and Ink4a are critical factors detemining the cell of origin and therapeutic of Myc-induced mouse lymphoid tumor (2011)
  • Author(s): Sugihara E, Kojima K, Ishizawa J, Andreeff M, Saya H
  • Organizer: 第53回アメリカ血液学会総会
  • Place of Presentation: 米国サンディエゴ国際会議場
• [Remarks]
  • URL: http://www.genereg.jp/
• [Remarks] 造血器腫瘍モデルを用いたがん幹細胞の性状解析・リンパ腫発症機構の解析
  • URL: http://www.genereg.jp/html/research/2013/04/post.html
• [Remarks]
  • URL: http://genereg.jp/index.html