Analysis of gastric and intestinal phenotype of gastric cancer in asymmetrical cell division

• Project/Area Number: 23790401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Hiroshima University
• Principal Investigator: OUE Naohide 広島大学, 医歯薬保健学研究院, 准教授 (60346484)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子病理 / 腫瘍 / 胃癌 / 形質発現 / 癌 / 病理学 / がん

Research Abstract

In the present study, we analyzed cancer stem cell marker and transition of gastric or intestinal phenotype in gastric cancer. GeneChip analysis revealed that KLF-2 and Ets-2, which are transcriptional factor, were over-expressed in side-population cells. We also found that integrin a2 isover-expressed in side-population cells. Fraction of side-population cell was reduced by knockdown of KLF-5 and Ets-2. Integrin a2 was expressed in cells showing perpendicular division. These molecules are potential marker for cancer stem cells.

Research Products

• [Journal Article] Expression of olfactomedin 4 and claudin-18 in serrated neoplasia of the colorectum: a characteristic pattern is associated with sessile serrated lesion (2013)
  • Author(s): Sentani K, Sakamoto N, Shimamoto F, Anami K, Oue N, Yasui W
  • Journal Title: Histopathology Volume: 62 Pages: 1018-27
  • Peer Reviewed
• [Journal Article] MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma (2013)
  • Author(s): Shinmei S, Sakamoto N, Goto K, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Kitadai Y, Yasui W
  • Journal Title: Int J Urol Volume: 20 Pages: 468-77
  • Peer Reviewed
• [Journal Article] The Search for Secreted Proteins in Prostate Cancer by the Escherichia coli Ampicillin Secretion Trap: Expression of NBL1 Is Highly Restricted to the Prostate and Is Related to Cancer Progression (2013)
  • Author(s): Hayashi T, Sentani K, Oue N, Ohara S, Teishima J, Anami K, Sakamoto N, Matsubara A, Yasui W
  • Journal Title: Pathobiology Volume: 80 Pages: 60-9
  • Peer Reviewed
• [Journal Article] NOS2 enhances KRAS-induced lung carcinogenesis, inflammation and microRNA-21 expression (2013)
  • Author(s): Okayama H, Saito M, Oue N, Weiss JM, Stauffer J, Takenoshita S, Wiltrout RH, Hussain SP, Harris CC
  • Journal Title: Int J Cancer Volume: 132 Pages: 9-18
  • Peer Reviewed
• [Journal Article] Expression of olfactomedin 4 and claudin-18 in serrated neoplasia of the colorectum: a characteristic pattern is associated with sessile serrated lesion. (2013)
  • Author(s): Sentani K, Sakamoto N, Shimamoto F, Anami K, Oue N, Yasui W.
  • Journal Title: Histopathology Volume: - Issue: 7 Pages: 1018-1027
  • DOI: 10.1111/his.12099
  • Peer Reviewed
• [Journal Article] The search for secreted proteins in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted to the prostate and is related to cancer progression. (2013)
  • Author(s): Hayashi T, Sentani K, Oue N, Ohara S, Teishima J, Anami K, Sakamoto N, Matsubara A, Yasui W.
  • Journal Title: Pathobiology Volume: 80 Issue: 2 Pages: 60-69
  • DOI: 10.1159/000341396
  • Peer Reviewed
• [Journal Article] Reg IV is a direct target of intestinal transcriptional factor CDX2 in gastric cancer. (2012)
  • Author(s): Naito Y, Oue N, Hinoi T, Sakamoto N, Sentani K, Ohdan H, Yanagihara K, Sasaki, H, Yasui W.
  • Journal Title: PLoS One Volume: 7(11) Issue: 11 Pages: 47545-47545
  • DOI: 10.1371/journal.pone.0047545
  • Peer Reviewed
• [Journal Article] Infantile adenomyoma subclinically excreted into the patient's diaper (2012)
  • Author(s): Anami K, Sentani K, Sakamoto N, Uraoka N, Oue N, Yasui W
  • Journal Title: Pathol Int Volume: 62 Pages: 532-7
  • Peer Reviewed
• [Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer (2012)
  • Author(s): Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Naito Y, Oo HZ, Hinoi T, Ohdan H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W
  • Journal Title: Cancer Sci Volume: 103 Pages: 1744-50
  • Peer Reviewed
• [Journal Article] Upregulation of HOXA10 in gastric cancer with the intestinal mucin phenotype: Reduction during tumor progression and favorable prognosis (2012)
  • Author(s): Sentani K, Oue N, Naito Y, Sakamoto N, Anami K, Oo HZ, Uraoka N, Aoyagi K, Sasaki H, Yasui W
  • Journal Title: Carcinogenesis Volume: 33 Pages: 1081-8
  • Peer Reviewed
• [Journal Article] Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD (2012)
  • Author(s): Gersemann M, Becker S, Nuding S, Antoni L, Ott G, Fritz P, Oue N, Yasui W, Wehkamp J, Stange EF
  • Journal Title: J Crohns Colitis Volume: 6 Pages: 425-34
  • Peer Reviewed
• [Journal Article] Cytokeratin 7 is a predictive marker for survival and effect of adjuvant chemotherapy in patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Oue N, Noguchi T, Anami K, Kitano S, Sakamoto N, Sentani K, Uraoka N, Aoyagi K, Yoshida T, Sasaki H, Yasui W
  • Journal Title: Ann Surg Oncol Volume: 19 Pages: 1902-10
  • Peer Reviewed
• [Journal Article] Cytokeratin expression profiling in gastric carcinoma: clinicopathologic significance and comparison with tumor-associated molecules (2012)
  • Author(s): Takami H, Sentani K, Matsuda M, Oue N, Sakamoto N, Yasui W
  • Journal Title: Pathobiology Volume: 79 Pages: 154-161
  • Peer Reviewed
• [Journal Article] Expression of cancer stem cell markers ALDH1, CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer (2012)
  • Author(s): Wakamatsu Y, Sakamoto N, Oo HZ, Naito Y, Uraoka N, Anami K, Sentani K, Oue N, Yasui W
  • Journal Title: Pathol Int Volume: 62 Pages: 112-119
  • Peer Reviewed
• [Journal Article] MicroRNA-155 is a predictive marker for survival in patients with clear cell renal cell carcinoma. (2012)
  • Author(s): Shinmei S, Sakamoto N, Goto K, Sentani K, Anami K, Hayashi T, Teishima J, Matsubara A, Oue N, Kitadai Y, Yasui W.
  • Journal Title: Int J Urol Volume: - Issue: 5 Pages: 468-477
  • DOI: 10.1111/j.1442-2042.2012.03182.x
  • Peer Reviewed
• [Journal Article] Decreased FANCJ caused by 5FU contributes to the increased sensitivity to oxaliplatin in gastric cancer cells. (2012)
  • Author(s): Mori R, Yoshida K, Tanahashi T, Yawata K, Kato J, Okumura N, Tsutani Y, Okada M, Oue N, Yasui W.
  • Journal Title: Gastric Cancer Volume: -
  • Peer Reviewed
• [Journal Article] Infantile adenomyoma subclinically excreted into the patient's diaper. (2012)
  • Author(s): Anami K, Sentani K, Sakamoto N, Uraoka N, Oue N, Yasui W.
  • Journal Title: Pathol Int Volume: 62 Issue: 8 Pages: 532-537
  • DOI: 10.1111/j.1440-1827.2012.02829.x
  • Peer Reviewed
• [Journal Article] Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer (2012)
  • Author(s): Sakamoto N, Oue N, Sentani K, Anami K, Uraoka N, Naito Y, Oo HZ, Hinoi T, Ohdan H, Yanagihara K, Aoyagi K, Sasaki H, Yasui W.
  • Journal Title: Liver-intestine cadherin induction by epidermal growth factor receptor is associated with intestinal differentiation of gastric cancer Volume: 103(9) Issue: 9 Pages: 1744-50
  • DOI: 10.1111/j.1349-7006.2012.02353.x
  • Peer Reviewed
• [Journal Article] NOS2 enhances KRAS-induced lung carcinogenesis, inflammation and microRNA-21 expression. (2012)
  • Author(s): Okayama H, Saito M, Oue N, Weiss JM, Stauffer J, Takenoshita S, Wiltrout RH, Hussain SP, Harris CC.
  • Journal Title: Int J Cancer Volume: 132 Issue: 1 Pages: 9-18
  • DOI: 10.1002/ijc.27644
  • Peer Reviewed
• [Journal Article] Upregulation of HOXA10 in gastric cancer with the intestinal mucin phenotype: reduction during tumor progression and favorable prognosis. (2012)
  • Author(s): Sentani K, Oue N, Naito Y, Sakamoto N, Anami K, Oo HZ, Uraoka N, Aoyagi K, Sasaki H, Yasui W.
  • Journal Title: Carcinogenesis Volume: 33 Issue: 5 Pages: 1081-1088
  • DOI: 10.1093/carcin/bgs121
  • Peer Reviewed
• [Journal Article] Olfactomedin-4 is a glycoprotein secreted into mucus in active IBD. (2012)
  • Author(s): Gersemann M, Becker S, Nuding S, Antoni L, Ott G, Fritz P, Oue N, Yasui W, Wehkamp J, Stange EF.
  • Journal Title: J Crohns Colitis Volume: 6 Issue: 4 Pages: 425-434
  • DOI: 10.1016/j.crohns.2011.09.013
  • Peer Reviewed
• [Journal Article] Cytokeratin expression profiling in gastric carcinoma: clinicopathologic significance and comparison with tumor-associated molecules. (2012)
  • Author(s): Takami H, Sentani K, Matsuda M, Oue N, Sakamoto N, Yasui W.
  • Journal Title: Pathobiology Volume: 79 Issue: 3 Pages: 154-161
  • DOI: 10.1159/000335694
  • Peer Reviewed
• [Journal Article] Expression of cancer stem cell markers ALDH1, CD44 and CD133 in primary tumor and lymph node metastasis of gastric cancer. (2012)
  • Author(s): Wakamatsu Y, Sakamoto N, Oo HZ, Naito Y, Uraoka N, Anami K, Sentani K, Oue N, Yasui W.
  • Journal Title: Pathol Int Volume: 62 Issue: 2 Pages: 112-119
  • DOI: 10.1111/j.1440-1827.2011.02760.x
  • Peer Reviewed
• [Journal Article] Cytokeratin 7 is a Predictive Marker for Survival in Patients with Esophageal Squamous Cell Carcinoma. (2012)
  • Author(s): Oue N, Noguchi T, Anami K, Kitano S, Sakamoto N, Sentani K, Uraoka N, Aoyagi K, Yoshida T, Sasaki H, Yasui W.
  • Journal Title: Ann Surg Oncol Volume: in press
  • Peer Reviewed
• [Journal Article] Desmocollin 2 is a new immunohistochemical marker indicative of squamous differentiation in urothelial carcinoma (2011)
  • Author(s): Hayashi T, Sentani K, Oue N, Anami K, Sakamoto N, Ohara S, Teishima J, Noguchi T, Nakayama H, Taniyama K, Matsubara A, Yasui W
  • Journal Title: Histopathology Volume: 59 Pages: 710-21
  • Peer Reviewed
• [Journal Article] Molecular pathology of gastric cancer: Research and practice (2011)
  • Author(s): Yasui W, Sentani K, Sakamoto N, Anami K, Naito Y, Oue N
  • Journal Title: Pathol Res Pract Volume: 207 Pages: 608-12
  • Peer Reviewed
• [Journal Article] Identification of transmembrane protein in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of CDON is involved in tumor cell growth and invasion (2011)
  • Author(s): Hayashi T, Oue N, Sakamoto N, Anami K, Oo HZ, Sentani K, Ohara S, Teishima J, Matsubara A, Yasui W
  • Journal Title: Pathobiology Volume: 78 Pages: 277-84
  • Peer Reviewed
• [Journal Article] Serum concentration and expression of Reg IV in patients with esophageal cancer: age-related elevation of serum Reg IV concentration (2011)
  • Author(s): Oue N, Noguchi T, Anami K, Sentani K, Sakamoto N, Uraoka N, Wakamatsu Y, Sasaki H and Yasui W
  • Journal Title: Oncol Lett Volume: 2(2) Pages: 235-239
  • Peer Reviewed
• [Journal Article] Cytokeratin 7 is a Predictive Marker for Survival in Patients with Esophageal Squamous Cell Carcinoma (2011)
  • Author(s): Naohide Oue, Tsuyoshi Noguchi, Wataru Yasui.(他8名, 2番目)
  • Journal Title: Ann Surg Oncol. Volume: 19 Issue: 6 Pages: 1902-1910
  • DOI: 10.1245/s10434-011-2175-4
  • Peer Reviewed
• [Journal Article] Desmocollin 2 is a newimmunohistochemical marker indicative ofsquamous differentiation in urothelialcarcinoma (2011)
  • Author(s): Hayashi T, Ohara S, Teishima J, MatsubaraA, Yasui W
  • Journal Title: Histopathology Volume: 59 Issue: 4 Pages: 710-721
  • DOI: 10.1111/j.1365-2559.2011.03988.x
  • Peer Reviewed
• [Journal Article] Molecular pathology of gastric cancer: research and practice. (2011)
  • Author(s): Yasui W, Sentani K, Sakamoto N, Anami K, Naito Y, Oue N.
  • Journal Title: Pathol Res Pract Volume: 207 Issue: 10 Pages: 608-612
  • DOI: 10.1016/j.prp.2011.09.006
  • Peer Reviewed
• [Journal Article] Identification of transmembrane protein in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of CDON is involved in tumor cell growth and invasion. (2011)
  • Author(s): Hayashi T, Oue N, Sakamoto N, Anami K, Oo HZ, Sentani K, Ohara S, Teishima J, Matsubara A, Yasui W.
  • Journal Title: Pathobiology Volume: 78 Pages: 277-284
  • Peer Reviewed
• [Journal Article] Combination of protein coding and non-coding gene expression as a robust prognostic classifier in stage I lung adenocarcinoma
  • Author(s): Akagi I, Okayama H, Schetter AJ, Robles AI, Kohno T, Bowman ED, Kazandjian D, Welsh JA, Oue N, Saito M, Miyashita M, Uchida E, Takizawa T, Takenoshita S, Skaug V, Mollerup S, Haugen A, Yokota J, Harris CC
  • Journal Title: Cancer Res Volume: (in press)
  • Peer Reviewed
• [Journal Article] Decreased FANCJ caused by 5FU contributes to the increased sensitivity to oxaliplatin in gastric cancer cells
  • Author(s): Mori R, Yoshida K, Tanahashi T, Yawata K, Kato J, Okumura N, Tsutani Y, Okada M, Oue N, Yasui W
  • Journal Title: Gastric Cancer Volume: (in press)
  • Peer Reviewed
• [Presentation] 食道扁平上皮癌におけるサイトケラチン7の免疫染色は術後補助化学療法の効果予 測マーカー、予後予測マーカーとして有用である (2012)
  • Author(s): 大上直秀
  • Organizer: 第23回日本消化器癌発生学会, ワークショップ 4
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-15
• [Presentation] Alteration of microRNA expression in gastrointestinal cancer. (2012)
  • Author(s): Oue N, Sakamoto N, Naito Y, Sentani K, Harris CC and Yasui W
  • Organizer: The 22nd Hiroshima Cancer Seminar and the 4th Japanese Association for RNA interference Joint International Symposium "MicroRNAs in cancer"
  • Place of Presentation: Hiroshima (Japan)
  • Year and Date: 2012-08-30
• [Presentation] SAGE 法、CAST 法から明らかになった胃型・腸型胃癌の生物学的・臨床的特徴 (2012)
  • Author(s): 大上直秀
  • Organizer: 第5回消化器 Research Seminar
  • Place of Presentation: 広島
  • Year and Date: 2012-06-19
• [Presentation] SAGE法、CAST法から明らかになった胃型・腸型胃癌の生物学的・臨床的特徴 (2012)
  • Author(s): 大上直秀
  • Organizer: 第5回消化器Research Seminar
  • Place of Presentation: 広島
  • Invited
• [Presentation] Alteration of microRNA expression in gastrointestinal cancer. (2012)
  • Author(s): Oue N, Sakamoto N, Naito Y, Sentani K, Harris CC and Yasui W
  • Organizer: The 22nd Hiroshima Cancer Seminar and the 4th Japanese Association for RNA interference Joint International Symposium “MicroRNAs in cancer”
  • Place of Presentation: Hiroshima
  • Invited
• [Presentation] 食道扁平上皮癌におけるサイトケラチン７の免疫染色は術後補助化学療法の効果予測マーカー、予後予測マーカーとして有用である (2012)
  • Author(s): 大上直秀、野口 剛、仙谷和弘、阿南勝宏、坂本直也、浦岡直礼、安井 弥
  • Organizer: 第23回日本消化器癌発生学会
  • Place of Presentation: 徳島
• [Presentation] 食道癌術後補助療法の効果予測マーカーとしてのサイトケラチン7 (2012)
  • Author(s): 大上直秀、仙谷和弘、阿南勝宏、坂本直也、浦岡直礼、安井 弥
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] 大腸癌パラフィン切片を用いたmiR-21の発現解析と薬剤感受性・予後との関連 (2012)
  • Author(s): 大上直秀、仙谷和弘、坂本直也、Aaron J. Schetter、安井 弥、Curtis C. Harris
  • Organizer: 第101回日本病理学会総会
  • Place of Presentation: 東京
• [Presentation] SPC18（SEC11A）によるEGF、TGF-aの分泌促進と胃癌における意義 (2012)
  • Author(s): 大上直秀、柳原五吉、内藤 寛、阿南勝宏、坂本直也、仙谷和弘、安井 弥
  • Organizer: 第21回日本がん転移学会学術集会・総会
  • Place of Presentation: 広島
• [Presentation] 食道扁平上皮癌に対するAdjuvant therapy効果予測因子としてのサイトケラチン7 (2012)
  • Author(s): 大上直秀、野口 剛、阿南勝宏、坂本直也、仙谷和弘、浦岡直礼、青柳一彦、吉田輝彦、佐々木博己、北野正剛、安井 弥
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] 病理セッション「胃癌における HER2 評価法と当研究室における経験」 (2011)
  • Author(s): 大上直秀
  • Organizer: 第2回 HER2 胃癌診断セミナー
  • Place of Presentation: 広島
  • Year and Date: 2011-09-08
• [Presentation] 消化器癌発生・進展と微小環境:SEC11A の胃癌における高発現と TGF-α/EGF 分泌促進作用 (2011)
  • Author(s): 大上直秀
  • Organizer: 第22回日本消化器癌発生学会, ワークショップ 1
  • Place of Presentation: 佐賀
• [Presentation] 胃癌トラスツズマブ病理部会作成のHERS ATRASの紹介と症例提示 (2011)
  • Author(s): 大上直秀
  • Organizer: 山陰病理Meeting(招待講演)
  • Place of Presentation: 米子
• [Presentation] 胃癌におけるHER2評価法と当研究室における経験 (2011)
  • Author(s): 大上直秀
  • Organizer: 第2回HER2胃癌診断セミナー(招待講演)
  • Place of Presentation: 広島
• [Presentation] 消化器癌発生・進展と微小環境：SEC11Aの胃癌における高発現とTGF-α/EGF分泌促進作用 (2011)
  • Author(s): 大上直秀、柳原五吉、内藤 寛、阿南勝宏、坂本直也、仙谷和弘、安井 弥
  • Organizer: 第22回日本消化器癌発生学会
  • Place of Presentation: 佐賀
• [Presentation] Overexpression of SEC11A is associated with tumor progression through TGF-alpha secretion in gastric cancer. (2011)
  • Author(s): Oue N, Yanagihara K, Anami K, Sentani K, Sakamoto N, Naito Y and Yasui W
  • Organizer: 8th International Symposium on Minimal Residual Cancer
  • Place of Presentation: Osaka
• [Presentation] SAGE-based microarrayにより同定したSEC11A（SPC18）はTGF-aの分泌を促進し胃癌の進行に関連している (2011)
  • Author(s): 大上直秀、林哲太郎、柳原五吉、若松雄太、内藤 寛、仙谷和弘、坂本直也、安井 弥
  • Organizer: 第100回日本病理学会総会
  • Place of Presentation: 横浜
• [Presentation] miR-21の発現は大腸癌の予後、薬剤感受性に関与する (2011)
  • Author(s): 大上直秀、Aaron J.Schetter、Markus Moehler、下村 学、檜井孝夫、青柳一彦、佐々木博己、岡島正純、大段秀樹、Peter R.Galle、安井 弥、Curtis C.Harris
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/byori1/
• [Remarks] 広島大学大学院医歯薬保健学研究院分子病理学研究室
  • URL: http://home.hiroshima-u.ac.jp/byori1/
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/byori1/
• [Patent(Industrial Property Rights)] 新規癌検出方法、癌検出器具、および癌検出キット (2012)
  • Inventor(s): 安井弥、大上直秀
  • Industrial Property Rights Holder: 国立大学法人広島大学
  • Acquisition Date: 2012-08-10