Establish of new deteciton of EGFR mutation status using immunostaining in non-small cell lung cancer

• Project/Area Number: 23790425
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Kurume University
• Principal Investigator: KAWAHARA Akihiko 久留米大学, 大学病院, 臨床検査技師 (00469347)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 病理学 / 免疫染色 / EGFR遺伝子変異抗体 / 癌 / 遺伝子検査 / 病理 / EGFR 遺伝子変異 / 免疫組織・細胞化学 / 分子病理学

Research Abstract

To establish a testing algorithm for EGFR mutation status in NSCLC patients, we utilized PNA-LNA PCR clamp assay and immunostaining. In the 133 samples, positive, negative and equivocal results for EGFR mutation-specific antibodies were observed in 37 patients (27.8%), 85 patients (63.9%) and 11 patients (8.3%), respectively. Thirty-seven patients showed positive EGFR expression by immunostaining, and 35 (94.6%) of these also tested positive in the DNA-based assay. Of 85 EGFR-negative patients by immunostaining, 77 (90.6%) also tested negative in the DNA-based assay. In NSCLC patients treated with EGFR-TKIs, the progression-free survival after the start of EGFR-TKIs treatment was significantly longer in patients with EGFR positive expression than in those with equivocal and negative expression (P = 0.002). Our results suggest immunostaining for EGFR mutation-specific antibodies are a new test for EGFR-TKI inhibition and could be a useful indicator with regard to patient management.

Research Products

• [Journal Article] Erlotinib resistance in lung cancer cells mediated by integrinβ1/Src/Akt-driven bypass signaling (2013)
  • Author(s): Kanda, R., Kage, M., Ono M, et al. 計12名
  • Journal Title: Cancer Res Volume: 73 Issue: 20 Pages: 6243-6253
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• [Journal Article] Prognostic Value of EGFR Mutation and ERCC1 in Patients with Non-Small Cell Lung Cancer Undergoing Platinum-Based Chemotherapy. (2013)
  • Author(s): Yamashita F, Azuma K, Yoshida T, Yamada K, Kawahara A, Hattori S, Takeoka H, Zaizen Y, Kawayama T, Kage M, Hoshino T.
  • Journal Title: PLoS ONE Volume: 5 Issue: 8 Pages: e71356-e71356
  • DOI: 10.1371/journal.pone.0071356
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• [Journal Article] Comparison of adverse events and efficacy between gefitinib and erlotinib in patients with non-small-cell lung cancer : a retrospective analysis (2013)
  • Author(s): Yoshida T, Yamada K, Azuma K, Kawahara A, Abe H, Hattori S, Yamashita F, Zaizen Y, Kage M, Hoshino T
  • Journal Title: Med Oncol Volume: 30巻 Issue: 1 Pages: 349-349
  • DOI: 10.1007/s12032-012-0349-y
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• [Journal Article] A case of mucoepidermoid carcinoma which could be diagnosed by liquid based bronchial brushing cytology (2013)
  • Author(s): 福満千容,河原明彦,山口知彦,安倍秀幸,多比良朋希,高瀬頼妃呼,秋葉純,鹿毛政義
  • Journal Title: The Journal of the Japanese Society of Clinical Cytology Volume: 52 Issue: 5 Pages: 473-477
  • DOI: 10.5795/jjscc.52.473
  • NAID: 10031191694
  • ISSN: 0387-1193, 1882-7233
  • Peer Reviewed
• [Journal Article] FOXP3 expression in tumor cells and tumor-infiltrating lymphocytes is associated with breast cancer prognosis (2013)
  • Author(s): Miki Takenaka, Naoko Seki, Uhi Toh, Satoshi Hattori, Akihiko Kawahara, Tomohiko Yamaguchi, Keiko Koura, Ryuji Takahashi, Hiroko Otsuka, Hiroki Takahashi, Nobutaka Iwakuma, Shino Nakagawa, Teruhiko Fujii, Tetsuro Sasada, Rin Yamaguchi, Hirohisa Yano, Kazuo Shirouzu, Masayoshi Kage
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 4 Pages: 625-632
  • DOI: 10.3892/mco.2013.107
  • Peer Reviewed
• [Journal Article] Clinicopathological significance of hypoxia-inducible factor-1 alpha (HIF-1α) expression in gastric cancer. (2013)
  • Author(s): Isobe T, Aoyagi K, Koufuji K, Shirouzu K, Kawahara A, Taira T, Kage M.
  • Journal Title: International journal of clinical oncology Volume: 18 Issue: 2 Pages: 293-304
  • DOI: 10.1007/s10147-012-0378-8
  • NAID: 10031176269
  • Peer Reviewed
• [Journal Article] Y-box binding protein-1 (YB-1) contributes to both HER2/ErbB2 expression and lapatinib sensitivity in human gastric cancer cells. (2013)
  • Author(s): Shibata T, Kan H, Murakami Y, Ureshino H, Watari K, Kawahara A, Kage M, Hattori S, Ono M, Kuwano M.
  • Journal Title: Molecular cancer therapeutics Volume: 12 Issue: 5 Pages: 737-746
  • DOI: 10.1158/1535-7163.mct-12-1125
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• [Journal Article] The relationship between 18F-FDG metabolic volumetric parameters and clinicopathological factors of breast cancer. (2013)
  • Author(s): Kaida H, Toh U, Hayakawa M, Hattori S, Fujii T, Kurata S, Kawahara A, Hirose Y, Kage M, Ishibashi M.
  • Journal Title: Nuclear medicine communications Volume: 34 Issue: 6 Pages: 562-570
  • DOI: 10.1097/mnm.0b013e328360d945
  • Peer Reviewed
• [Journal Article] Expression of Monocarboxylate Transporter (MCT)-4 in Colorectal Cancer and its Role: MCT4 Contributes to the Growth of Colorectal Cancer with Vascular Endothelial Growth Factor. (2013)
  • Author(s): Gotanda Y, Akagi Y, Kawahara A, Kinugasa T, Yoshida T, Ryu Y, Shiratsuchi I, Kage M, Shirouzu K.
  • Journal Title: Anticancer research Volume: 33 Pages: 2941-2947
  • Peer Reviewed
• [Journal Article] N-myc downstream-regulated gene 1 promotes tumor inflammatory angiogenesis through JNK activation and autocrine loop of interleukin-1α by human gastric cancer cells (2013)
  • Author(s): Murakami, Y., Watari, K., Ono, M, et al. 計11名
  • Journal Title: J Biol Chem Volume: 288 Issue: 35 Pages: 25025-25037
  • DOI: 10.1074/jbc.m113.472068
  • Peer Reviewed
• [Journal Article] A case of metastasizing pleomorphic adenoma in the maxillary bone appearing twenty years after initial resection of pleomorphic adenoma of the hard palate. (2013)
  • Author(s): Akiba J, Harada H, Kawahara A, Todoroki K, Nagata S, Yano H.
  • Journal Title: Pathology international Volume: 63 Issue: 9 Pages: 463-368
  • DOI: 10.1111/pin.12087
  • Peer Reviewed
• [Journal Article] Phase II study of personalized peptide vaccination for refractory bone and soft tissue sarcoma patients. (2013)
  • Author(s): Takahashi R, Ishibashi Y, Hiraoka K, Matsueda S, Kawano K, Kawahara A, Kage M, Ohshima K, Yamanaka R, Shichijo S, Shirouzu K, Itoh K, Sasada T.
  • Journal Title: Cancer Sci. Volume: 104 Issue: 10 Pages: 1285-1294
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• [Journal Article] Treatment outcome in patients with stage III breast cancer treated with neoadjuvant chemotherapy. (2013)
  • Author(s): Takahashi R, Toh U, Iwakuma N, Mishima M, Fujii T, Takenaka M, Koura K, Seki N, Kawahara A, Kage M, Ogo E, Shirouzu K.
  • Journal Title: Experimental and therapeutic medicine Volume: 6 Issue: 5 Pages: 1089-1095
  • DOI: 10.3892/etm.2013.1289
  • Peer Reviewed
• [Journal Article] Potential usefulness of mucin immunohistochemical staining of preoperative pancreatic biopsy or juice cytology specimens in the determination of treatment strategies for intraductal papillary mucinous neoplasm. (2013)
  • Author(s): Hisaka T, Horiuchi H, Uchida S, Ishikawa H, Kawahara R, Kawashima Y, Akashi M, Mikagi K, Ishida Y, Okabe Y, Nakayama M, Naito Y, Yano H, Taira T, Kawahara A, Kage M, Kinoshita H, Shirozu K.
  • Journal Title: Oncology Reports Volume: 30 Issue: 5 Pages: 2035-2041
  • DOI: 10.3892/or.2013.2720
  • Peer Reviewed
• [Journal Article] A diagnostic algorithm using EGFR mutation-specific antibodies for rapid response EGFR-TKI treatment in patients with non-small cell lung cancer (2012)
  • Author(s): Kawahara A, Taira T, Azuma K, Tominaga M, Hattori S, Kawahara M, Abe H, Yamaguchi T, Akiba J, Takamori S, Hayashi A, Kage M
  • Journal Title: Lung Cancer Volume: 78巻 Issue: 1 Pages: 39-44
  • DOI: 10.1016/j.lungcan.2012.07.002
  • Peer Reviewed
• [Journal Article] A case of primary lung cancer with EGFR mutation which needed to be distinguished from lung metastasis of breast cancer (2012)
  • Author(s): 河原明彦,山口知彦,安倍秀幸,多比良朋希,吉田友子,内藤嘉紀,秋葉純,鹿毛政義
  • Journal Title: The Journal of the Japanese Society of Clinical Cytology Volume: 51 Issue: 2 Pages: 120-124
  • DOI: 10.5795/jjscc.51.120
  • NAID: 130004513125
  • ISSN: 0387-1193, 1882-7233
  • Peer Reviewed
• [Journal Article] Chemotherapy for non-small cell lung cancer complicated by idiopathic interstitial pneumonia (2012)
  • Author(s): Kinoshita T, Azuma K, Sasada T, Okamoto M, Hattori S, Imamura Y, Yamada K, Tajiri M, Yoshida T, Zaizen Y, Kawahara A, Fujimoto K, Hoshino T
  • Journal Title: Oncol Lett Volume: 4巻 Issue: 3 Pages: 477-482
  • DOI: 10.3892/ol.2012.753
  • Peer Reviewed
• [Journal Article] Prognostic significance of total lesion glycolysis in patients with advanced non-small cell lung cancer receiving chemotherapy. (2012)
  • Author(s): Zaizen Y, Azuma K, Kurata S, Sadashima E, Hattori S, Sasada T, Imamura Y, Kaida H, Kawahara A, Kinoshita T, Ishibashi M, Hoshino T.
  • Journal Title: Eur J Radiol. Volume: 81 Issue: 12 Pages: 4179-84
  • DOI: 10.1016/j.ejrad.2012.07.009
  • Peer Reviewed
• [Journal Article] Loss of Activating EGFR Mutant Gene Contributes to Acquired Resistance to EGFR Tyrosine Kinase Inhibitors in Lung Cancer Cells (2012)
  • Author(s): Tabara K, Kanda R, Sonoda K, Kubo T, Murakami Y, Kawahara A, Azuma K, Abe H, Kage M, Yoshinaga A, Tahira T, Hayashi K, Arao T, Nishio K, Rosell R, Kuwano M, Ono M
  • Journal Title: PLoS One Volume: 7巻 Issue: 7 Pages: e41017-e41017
  • DOI: 10.1371/journal.pone.0041017
  • Peer Reviewed
• [Journal Article] NDRG1/Cap43/Drg-1 may predict tumor angiogenesis and poor outcome in patients with lung cancer (2012)
  • Author(s): Azuma, K., Kawahara, A., Hattori, S., Taira, T., Tsurutani, J., Watari, K., Shibata, T., Murakami, Y., Takamori, S., Ono, M., Izumi, H., Kage, M., Yanagawa, T., Nakagawa, K., Hoshino, T., Kuwano, M.
  • Journal Title: Journal of Thoracic Oncology Volume: (In press) Issue: 5 Pages: 779-789
  • DOI: 10.1097/jto.0b013e31824c92b4
  • Peer Reviewed
• [Journal Article] Immunological evaluation of personalized peptide vaccination in refractory small cell lung cancer. (2012)
  • Author(s): Terazaki Y, Yoshiyama K, Matsueda S, Watanabe N, Yamada A, Mine T, Terasaki M, Itoh K, Takamori S, Sasada T.
  • Journal Title: Cancer Sci Volume: 103(4) Issue: 4 Pages: 638-644
  • DOI: 10.1111/j.1349-7006.2012.02202.x
  • Peer Reviewed
• [Journal Article] A diagnostic algorithm using EGFR mutation-specific antibodies for rapid response EGFR-TKI treatment in patients with non-small cell lung cancer. (2012)
  • Author(s): Kawahara A, Taira T, Azuma K, Tominaga M, Hattori S, Kawahara M, Abe H, Yamaguchi T, Akiba J, Takamori S, Hayashi A, Kage M.
  • Journal Title: Lung Cancer. Volume: 78 Pages: 39-44
  • Peer Reviewed
• [Journal Article] Association of the expression of mutant epidermal growth factor receptor protein as determined with mutation-specific antibodies in non-small cell lung cancer with progression-free survival after gefitinib treatment (2011)
  • Author(s): Azuma, K., Okamoto, I., Kawahara, A., Taira, T., Nakashima, K., Hattori. S., Kinoshita, T., Takeda, M., Nakagawa, K., Takamori, S., Kuwano, M., Ono, M., Kage, M.
  • Journal Title: Journal of Thoracic Oncology Volume: 6 Issue: 1 Pages: 122-127
  • DOI: 10.1097/jto.0b013e31822eeba2
  • Peer Reviewed
• [Journal Article] Identification of non-small-cell lung cancer with activating EGFR mutations in malignant effusion and cerebrospinal fluid : Rapid and sensitive detection of exon 19 deletion E746-A750 and exon 21 L858R mutation by immunocytochemistry (2011)
  • Author(s): Kawahara A, Azuma K, Sumi A, Taira T, Nakashima K, Aikawa E, Abe H, Yamaguchi T, Takamori S, Akiba J, Kage M
  • Journal Title: Lung Cancer Volume: 74巻 Issue: 1 Pages: 35-40
  • DOI: 10.1016/j.lungcan.2011.02.002
  • Peer Reviewed
• [Journal Article] Comparison of the expression levels of napsin A, thyroid transcription factor-1, and p63 in nonsmall cell lung cancer using cytocentrifuged bronchial brushings (2011)
  • Author(s): Aikawa E, Kawahara A, Hattori S, Yamaguchi T, Abe H, Taira T, Azuma K, Kage M
  • Journal Title: Cancer Cytopathol Volume: 119巻 Issue: 5 Pages: 335-340
  • DOI: 10.1002/cncy.20162
  • Peer Reviewed
• [Journal Article] Identification of non-small-cell lung cancer with activating EGFR mutations in malignant effusion and cerebrospinal fluid: Rapid and sensitive detection of exon 19 deletion E746-A750 and exon 21 L858R mutation by immunocytochemistry. (2011)
  • Author(s): Kawahara A.
  • Journal Title: Lung Cancer. Volume: 73 Pages: 35-40
  • Peer Reviewed
• [Presentation] Integrin beta1/Src/Akt-driven Bypass Signaling Pathway confers EGFR-TKI Resistance in Lung Cancer Cells (2013)
  • Author(s): Mayumi Ono, Rina Kanda, Kosuke Watari, Kahori Sonoda, Masashi, Maeda, Yuichi Murakami, Akihiko Kawahara, Masayoshi Kage, Hidetaka Uramoto, Michihiko Kuwano
  • Organizer: 72th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
  • Year and Date: 2013-10-03
• [Presentation] A case of EML4-ALK-positive lung adenocarcinoma with signet-ring cells (2013)
  • Author(s): Akihiko Kawahara, Tomoki Taira, Tomohiko Yamaguchi, Hideyuki Abe, Tomoko Yoshida, Yorihiko Takase, Chihiro Fukumitsu, Jun Akiba, Kage Masayoshi
  • Organizer: The 12^<th > Korea-Japan Joint Meeting for Diagnostic Cytopathology
  • Place of Presentation: 韓国
  • Year and Date: 2013-09-07
• [Presentation] Integrinβ1/Akt経路活性化を介した肺癌における新規エルロチニブ耐性獲得メカニズム (2013)
  • Author(s): 神田利奈,渡公祐,河原明彦,鹿毛政義,浦本秀隆,岡本勇,中川和彦,桑野信彦,小野眞弓
  • Organizer: 第17回日本がん分子標的治療学会
  • Place of Presentation: 京都
  • Year and Date: 2013-06-13
• [Presentation] 液状気管支細胞診を用いた肺癌診断 (2013)
  • Author(s): 福満千容,河原明彦,多比良朋希,山口知彦,安倍秀幸,吉田友子,髙瀨頼妃呼,秋葉純,谷川健,鹿毛政義
  • Organizer: 第54回日本臨床細胞学会総会
  • Place of Presentation: 東京
  • Year and Date: 2013-06-02
• [Presentation] LBCを用いた気管支擦過細胞診の現状.肺癌治療における細胞診の役割 (2013)
  • Author(s): 河原明彦
  • Organizer: 中四国実践セミナー
  • Place of Presentation: 愛媛
  • Year and Date: 2013-03-09
• [Presentation] Glucose transporter-1(GLUT-1) expression in endometrial lesions. (2013)
  • Author(s): Tomohiko YAMAGUCHI, Akihiko KAWAHARA, Tomoki TAIRA, Hideyuki ABE, Yoshiki NAITO, Sakiko SANADA, Shunichiro OTA, Kimio USHIJIMA, Toshiharu KAMURA, Masayoshi KAGE.
  • Organizer: 18th International Congress of Cytology
  • Place of Presentation: Paris
• [Presentation] 唾液腺領域における良性腫瘍を悪性と診断しない鑑別ポイント． (2013)
  • Author(s): 河原明彦，原田博史，山口知彦，安倍秀幸，多比良朋希，髙瀨頼妃呼，福満千容，谷川 健， 秋葉 純，鹿毛政義
  • Organizer: 第54回日本臨床細胞学会総会
  • Place of Presentation: 東京
• [Presentation] 液状気管支細胞診を用いた肺癌診断． (2013)
  • Author(s): 福満千容，河原明彦，多比良朋希，山口知彦，安倍秀幸，吉田友子，髙瀨頼妃呼，秋葉 純，谷川 健，鹿毛政義．
  • Organizer: 第54回日本臨床細胞学会総会
  • Place of Presentation: 東京
• [Presentation] 細胞診による子宮内膜癌のtype分類の検討． (2013)
  • Author(s): 山口知彦，河原明彦，多比良朋希，安部秀幸，吉田友子，真田咲子，秋葉 純，牛島公生，嘉村敏治，鹿毛政義
  • Organizer: 第54回日本臨床細胞学会総会
  • Place of Presentation: 東京
• [Presentation] Integrinβ1/Akt経路活性化を介した肺癌における新規エルロチニブ耐性獲得メカニズム． (2013)
  • Author(s): 神田利奈，渡 公祐，河原明彦，鹿毛政義，浦本秀隆，岡本 勇，中川和彦，桑野信彦，小野眞弓．
  • Organizer: 第17回日本がん分子標的治療学会
  • Place of Presentation: 京都
• [Presentation] Y-box結合タンパク(YB-1)はヒト胃癌においてHER2/ErbB2発現とラパチニブの感受性を制御する (2013)
  • Author(s): 柴田智博，菅 仁史，村上雄一，渡 公祐，河原明彦，鹿毛政義，和泉弘人，桑野信彦，小野眞弓．
  • Organizer: 第17回日本がん分子標的治療学会
  • Place of Presentation: 京都
• [Presentation] A case of EML4-ALK-positive lung adenocarcinoma with signet-ring cells. (2013)
  • Author(s): Akihiko Kawahara, Tomoki Taira, Tomohiko Yamaguchi, Hideyuki Abe, Tomoko Yoshida, Yorihiko Takase, Chihiro Fukumitsu, Jun Akiba, Kage Masayoshi.
  • Organizer: The 12th Korea-Japan Joint Meeting for Diagnostic Cytopathology.
  • Place of Presentation: Korea
• [Presentation] Claudin-1 is detection marker of squamoid corpuscles in pancreatoblastoma. (2013)
  • Author(s): Tomoki Taira, Akihiko Kawahara, Tomohiko Yamaguchi, Hideyuki Abe, Tomoko Yoshida, Yorihiko Takase, Chihiro Fukumitsu, Jun Akiba, Kage Masayoshi.
  • Organizer: The 12th Korea-Japan Joint Meeting for Diagnostic Cytopathology.
  • Place of Presentation: Korea
• [Presentation] Integrin beta1/Src/Akt-driven Bypass Signaling Pathway confers EGFR-TKI Resistance in Lung Cancer Cells. (2013)
  • Author(s): Mayumi Ono, Rina Kanda, Kosuke Watari, Kahori Sonoda, Masashi, Maeda, Yuichi Murakami, Akihiko Kawahara, Masayoshi Kage, Hidetaka Uramoto, Michihiko Kuwano.
  • Organizer: 72th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
• [Presentation] NDRG1 deficiency suppresses tumor angiogenesis through interference of macrophage activation. (2013)
  • Author(s): Kosuke Watari, Hiroshi Nabeshima, Yuichi Fukunaga, TomohiroShibata , Ai Shinoda, Akihiko Kawahara, Yuichi Murakami, Masayoshi Kage, Michihiko Kuwano, Mayumi Ono.
  • Organizer: 72th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
• [Presentation] IL-1α-driven AP-1 (Jun /Fos) activation plays a key role in inflammatory angiogenesis by NDRG1 in gastric cancer. (2013)
  • Author(s): Yuichi Murakami, Kosuke Watari, Tomohiro Shibata, Hiroki Ureshino, Akihiko Kawahara, Masayoshi Kage, Hiroto Izumi, Michihiko Kuwano, Mayumi Ono.
  • Organizer: 72th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Yokohama
• [Presentation] 腫瘍性筋上皮細胞が関与する唾液腺腫瘍の診断アプローチ．第52回 (2013)
  • Author(s): 12. 河原明彦，原田博史，多比良朋希，山口知彦，安部秀幸，吉田友子，髙瀨頼妃呼，福満千容，秋葉 純，鹿毛政義．
  • Organizer: 第52回日本臨床細胞学会秋期大会
  • Place of Presentation: 大阪
• [Presentation] ワルチン腫瘍と粘表皮癌におけるp63の発現比較． (2013)
  • Author(s): 高橋瑞恵，河原明彦，佐藤真介，宮崎浩子，横地康輔，中島明彦．
  • Organizer: 第52回日本臨床細胞学会秋期大会
  • Place of Presentation: 大阪
• [Presentation] 中間型松果体実質腫瘍の1例． (2013)
  • Author(s): 安部秀幸，河原明彦，杉田保雄，山口知彦，多比良朋希，吉田友子，髙瀨頼妃呼，福満千容，鹿毛政義．
  • Organizer: 第29回日本臨床細胞学会福岡県支部総会
  • Place of Presentation: 北九州
• [Presentation] 液状検体における細胞診の役割と遺伝子診断 (2012)
  • Author(s): 河原明彦,多比良朋希,山口知彦,安倍秀幸,吉田友子,髙瀨頼妃呼,福満千容,秋葉純,鹿毛政義
  • Organizer: 第51回日本臨床細胞学会秋期大会
  • Place of Presentation: 新潟
  • Year and Date: 2012-11-10
• [Presentation] 液状細胞診にて診断した気管支原発粘表皮癌の1例 (2012)
  • Author(s): 福満千容,河原明彦,山口知彦,安倍秀幸,多比良朋希,吉田友子,髙瀨頼妃呼,秋葉純,鹿毛政義
  • Organizer: 第51回日本臨床細胞学会秋期大会
  • Place of Presentation: 新潟
  • Year and Date: 2012-11-10
• [Presentation] Identification of non-small-cell lung cancer with activating EGFR mutations in malignant effusion and cerebrospinal fluid (2012)
  • Author(s): Kawahara Akihiko
  • Organizer: 37^<th> European Congress of Cytology
  • Place of Presentation: Croatia, Dubrovnik
  • Year and Date: 2012-09-30
• [Presentation] Interleukin 1 promotes lymph node metastasis by lung cancer cells through modification of tumor stroma cells (2012)
  • Author(s): Tomohiro Shibata, Kosuke Watari, Akihiko Kawahara, Yuichi Murakami, Hiroshi Nabeshima, Koichi Azuma, Masayoshi Kage, Naofumi Mukaida, Michihiko Kuwano, Takashi Takahashi, Mayumi Ono
  • Organizer: 71th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 71th Annual Meeting of the Japanese Cancer Association
  • Year and Date: 2012-09-21
• [Presentation] A novel mechanism for acquired resistance to EGFR-TKI in human lung cancer : loss of activating mutant EGFR gene allele (2012)
  • Author(s): Rina Kanda, Yuichi Murakami, Akihiko Kawahara, Koichi Azuma, Masayoshi Kage, Tomoko Tahira, Michihiko Kuwano, Mayumi Ono
  • Organizer: 71th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Hokkaido
  • Year and Date: 2012-09-21
• [Presentation] Characteristics of immunohistochemical ALK-positive lung cancer (2012)
  • Author(s): Jun Akiba, Akihiko Kawahara, Masayoshi Kage, Hirohisa Yano
  • Organizer: 71th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Hokkaido
  • Year and Date: 2012-09-19
• [Presentation] 液状検体における細胞診の役割と遺伝子診断． (2012)
  • Author(s): 河原明彦，多比良朋希，山口知彦，安倍秀幸，吉田友子，髙瀨頼妃呼，福満千容，秋葉 純， 鹿毛政義．
  • Organizer: 第51回日本臨床細胞学会秋期大会
  • Place of Presentation: 新潟
• [Presentation] ワークショップ.肺癌患者の診断と治療における呼吸器細胞診の役割 (2011)
  • Author(s): 河原明彦,多比良朋希,安部秀幸,山口知彦,吉田友子,秋葉純,鹿毛政義
  • Organizer: 第50回日本臨床細胞学会秋期大会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-23
• [Presentation] Immunocytochemical diagnosis of activating EGFR mutations in NSCLC using mutation-specific antibodies (2011)
  • Author(s): Kawahara Akihiko, Koichi Azuma, Kuwano Michihiko, Masayoshi Kage, Mayumi Ono
  • Organizer: 70^<th> Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] 再発肺癌細胞におけるEGFR遺伝子変異の免疫細胞化学による検出 (2011)
  • Author(s): 河原明彦,山口知彦,安倍秀幸,多比良朋希,吉田友子,内藤嘉紀,秋葉純,鹿毛政義
  • Organizer: 第52回日本臨床細胞学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2011-05-21
• [Presentation] Immunocytochemical diagnosis of activating EGFR mutations in NSCLC using mutation-specific antibodies. (2011)
  • Author(s): Akihiko Kawahara.
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association.
  • Place of Presentation: 名古屋
• [Presentation] 肺癌患者の診断と治療における呼吸器細胞診の役割. (2011)
  • Author(s): 河原明彦.
  • Organizer: 第50回日本臨床細胞学会秋期大会
  • Place of Presentation: 東京