Regulation of JC virus late gene expression

• Project/Area Number: 23790429
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: ORBA Yasuko 北海道大学, 人獣共通感染症リサーチセンター, 特任助教 (60507169)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ＪＣウイルス / RNA / 脳神経疾患 / JCウイルス / ウイルス

Research Abstract

JC virus(JCV) late transcripts, which produce agnoprotein, VP1, VP2, and VP3, are polycistronic mRNAs and processed by alternative splicing. We found that JCV late transcripts are processed into mature mRNAs using a unique splicing mechanism. Analysis of virus infection using JCV genomes with splice site mutations indicated that the identified splicing mechanism is necessary for efficient production of late proteins and viral infection.

Research Products

• [Journal Article] Viroporin activity of the JC polyomavirus is regulated by interactions with the adaptor protein complex 3. (2013)
  • Author(s): Suzuki T, Orba Y, Makino Y, Okada Y, Sunden Y, Hasegawa H, Hall WW, Sawa H.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 12;110(46) Issue: 46 Pages: 18668-73
  • DOI: 10.1073/pnas.1311457110
  • NAID: 120005429604
  • Peer Reviewed
• [Journal Article] Relationship between Methyl CpG Binding Protein 2 and JC Viral Proteins (2013)
  • Author(s): Takahashi K, Orba Y, Kimura T, Wang L, Kohsaka S, Tsuda M, Tanino M, Nishihara H, Nagashima K, Sawa H, Tanaka S
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 66 Issue: 2 Pages: 126-132
  • DOI: 10.7883/yoken.66.126
  • NAID: 130003381654
  • ISSN: 1344-6304, 1884-2836
  • Peer Reviewed
• [Journal Article] Identification of a novel polyomavirus from vervet monkeys in Zambia. (2013)
  • Author(s): Yamaguchi H, Kobayashi S, Ishii A, Ogawa H, Nakamura I, Moonga L, Hang'ombe BM, Mweene AS, Thomas Y, Kimura T, Sawa H, Orba Y.
  • Journal Title: J Gen Virol. Volume: 94(Pt 6) Issue: 6 Pages: 1357-64
  • DOI: 10.1099/vir.0.050740-0
  • Peer Reviewed
• [Journal Article] Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation (2013)
  • Author(s): Kobayashi S, Suzuki T, Igarashi M, Orba Y, Ohtake N, Nagakawa K, Niikura K, Kimura T, Kasamatsu H, Sawa H
  • Journal Title: PLoS One Volume: 8(10) Issue: 10 Pages: e76668-e76668
  • DOI: 10.1371/journal.pone.0076668
  • NAID: 120005350093
  • Peer Reviewed
• [Journal Article] Relationship between methyl CpG binding protein 2 and JC viral proteins. (2013)
  • Author(s): Takahashi K, Orba Y, Kimura T, Wang L, Kohsaka S, Tsuda M, Tanino M, Nishihara H, Nagashima K, Sawa H, Tanaka S.
  • Journal Title: Jpn J Infect Dis. Volume: 66(2) Pages: 126-32
  • NAID: 130003381654
  • Peer Reviewed
• [Journal Article] Role of JC virus agnoprotein in virion formation (2012)
  • Author(s): Suzuki T, Semba S, Sunden Y, Orba Y, Kobayashi S, Nagashima K, Kimura T, Hasegawa H, Sawa H
  • Journal Title: Microbiol Immunol Volume: 56(9) Pages: 639-646
  • Peer Reviewed
• [Journal Article] Accumulation of ubiquitinated proteins is related to the West Nile virus-induced neuronal apoptosis (2012)
  • Author(s): Kobayashi S, Orba Y, Yamaguchi H, Kimura T, Sawa H
  • Journal Title: Neuropathology Volume: 32(4) Issue: 4 Pages: 398-405
  • DOI: 10.1111/j.1440-1789.2011.01275.x
  • Peer Reviewed
• [Journal Article] Role of JC virus agnoprotein in virion formation. (2012)
  • Author(s): Suzuki T, Semba S, Sunden Y, Orba Y, Kobayashi S, Nagashima K, Kimura T, Hasegawa H, Sawa H.
  • Journal Title: Microbiol Immunol Volume: 56(9) Issue: 9 Pages: 639-46
  • DOI: 10.1111/j.1348-0421.2012.00484.x
  • Peer Reviewed
• [Journal Article] Detection and characterization of a novel polyomavirus in wild rodents. (2011)
  • Author(s): Orba Y, Kobayashi S, Nakamura I, Ishii A, Hang'ombe BM, Mweene AS, Thomas Y, Kimura T, Sawa H.
  • Journal Title: Journal of General Virology Volume: vol.92 no.4 Issue: 4 Pages: 789-795
  • DOI: 10.1099/vir.0.027854-0
  • Peer Reviewed
• [Presentation] JCウイルスT抗原C末端領域の機能解析 (2013)
  • Author(s): 大場靖子
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 2013-11-10
• [Presentation] JCウイルスT抗原C末端領域の機能解析 (2013)
  • Author(s): 大場靖子、木村享史、澤洋文
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場(神戸市）
• [Presentation] ザンビアの野生動物におけるオルソポックスウイルス感染の疫学調査 (2012)
  • Author(s): 大場靖子、石井秋宏、トーマス由佳、小川寛人、中村一郎、木村享史、森川茂、西條政幸、澤洋文
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪府）
• [Remarks]
  • URL: http://www.czc.hokudai.ac.jp/index.html
• [Remarks] 北海道大学人獣共通感染症リサーチセンター 分子病態・診断部門ホームページ
  • URL: http://www.czc.hokudai.ac.jp/pathobiol/