A Establishment of a leukemina model of cynomolgus macaque
Project/Area Number |
23790442
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | Shiga University of Medical Science |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | iPS細胞 / 白血病 / 動物モデル / 動物実験モデル / カニクイザル / 白血病幹細胞 / レトロウイルス / 再生医療 / 主要組織適合抗原 |
Research Abstract |
We successfully induced CD34 positive hematopoietic stem cells (iCD34) from induced pluripotent stem cells (iPS cells) of a cynomolgus macaque which homozygously have a certain haplotype of major histocompatibility complex (Mafa-HT1). Next, we transduced well-known leukemic genes, AML1/ETO and FLT3mutant, or BCR/ABL, to the iCD34 by retrovirus. These two types of iCD34 (leukemic iCD34) cells with the leukemic genes could form many colonies in methylcellulose media and be cultured for long term without any cytokines. These leukemic iCD34 could be alive in NOD/Shi-scid, IL-2Rγnull mouse (NOG mouse) after intrabonemarrow-bonemarrow transplantation (IBM-BMT). We are preparing experiments in which a bonemarrow transplantation of the leukemic iCD34 with AML1/ETO+FLT3mutant to a cynomolgusmacaque with heterogygous MHC.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A*052:022012
Author(s)
Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Efficacy of single intravenous injection of peramivir against influenza B virus infection in ferrets and cynomolgus macaques2011
Author(s)
Kitano M, Itoh Y, Kodama M, Ishigaki H, Nakayama M, Ishida H, Baba K, Noda T, Sato K, Nihashi Y, Kanazu T, Yoshida R, Torii R, Sato A, Ogasawara K
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Journal Title
Antimicrobial Agents and Chemotherapy
Volume: Vol.55
Issue: 11
Pages: 4961-4970
DOI
Related Report
Peer Reviewed
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