Development of a novel orthotopic transplantation model of human small cell lung cancer metastasis.
| Project/Area Number |
23790452
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Experimental pathology
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
SAKAMOTO Shuichi 公益財団法人微生物化学研究会, 微生物化学研究所 沼津支所, 主任研究員 (60346070)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 癌転移 / 小細胞肺癌 / 同所移植 / HGF / 自然転移モデル / ヌードマウス / 転移 / 足場非依存性増殖能 / マトリゲル浸潤能 |
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| Research Abstract |
Because small cell lung cancer (SCLC) is an aggressive cancer with highly metastatic activity, it is important to reveal the molecular basis of the SCLC metastasis for improvement of SCLC therapeutics. In this study, we developed a new in vivo orthotopic model for SCLC metastasis and investigated the molecular mechanism of metastasis using the model. We found that cells used in our model expressed both HGF and its receptor c-MET, and that condition medium of the cells enhanced motility of them. These data suggest the existence of an HGF/MET autocrine loop in our model as in SCLC patients.
Moreover, we established a new subclone of the cells with higher metastatitic activity through in vivo selection cycles, and successfully improved our model using this subclone.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells via activation of retinoic acid receptor gamma.2012
Author(s)
Sakamoto, S., Kojima, F., Momose, I., Kawada, M., Adachi, H., and Nishimura, Y.
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Journal Title
Biochem. Biophys. Res. Commun.
Volume: 422
Issue: 4
Pages: 751-757
DOI
Related Report
Peer Reviewed
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