Researching the mechanism of cancer-associated epithelial cells

• Project/Area Number: 23790455
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: National Cancer Center Japan
• Principal Investigator: SHIRAKIHARA Takuya 独立行政法人国立がん研究センター, 研究所, 研究員 (30548756)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細胞 / EMT / 癌微小環境 / TGF-β / FGF-2 / TGF-beta

Research Abstract

We have previously reported that EMT-acquired normal epithelial cells facilitated invasion of cancer cells, when they mixed and cultured together. A number of specific genes expressed in these cells were identified by using microarray analysis. ITGA3 was highly expressed in poorly-differentiated breast cancer tissues and the neutralizing antibody of ITGA3 suppressed invasion of EMT-acquired cells.

Research Products

• [Journal Article] Identification of integrin alpha3, as a molecular maker of cells undergoing EMT and cancer cells with aggressive phenotypes
  • Author(s): Takuya Shirakihara, Tomonori Kawasaki, Akihito Fukagawa, Kentaro Semba, Ryuichi Sakai, Kohei Miyazono, Keiji Miyazawa, Masao Saitoh
  • Journal Title: Cancer Science Volume: (未掲載)
  • Peer Reviewed
• [Presentation] FGF-2 accelerates TGF-beta-induced EMT and promotes invasion of cancer (2013)
  • Author(s): 白木原 琢哉、堺 隆一、齋藤 正夫
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-10-03
• [Presentation] FGF-2 accelerates TGF-beta-induced EMT and promotes invasion of cancer (2013)
  • Author(s): Takuya Shirakihara, Ryuichi Sakai, Masao Saitoh
  • Organizer: The 3^<rd> Symposium Mechanisms and Models of Cancer
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2013-08-07
• [Presentation] FGF-2によるTGF-beta誘導性EMTの亢進とがん浸潤への関与 (2013)
  • Author(s): 白木原 琢哉、堺 隆一、齋藤 正夫
  • Organizer: 第22回日本がん転移学会学術集会・総会
  • Place of Presentation: 松本
  • Year and Date: 2013-07-11
• [Presentation] FGF-2によるTGF-β誘導性EMTの亢進とがん浸潤への関与 (2013)
  • Author(s): 白木原 琢哉
  • Organizer: 第22回日本がん転移学会学術集会・総会
  • Place of Presentation: 松本
• [Presentation] FGF-2 accelerates TGF-β-induced epithelial-mesenchymal transition and p romotes invasion of cancer (2013)
  • Author(s): 白木原 琢哉
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] TGF-beta swiches isoform of FGF receptors and regulates EMT of cancer-associated epithelial cells (2011)
  • Author(s): Takuya Shirakihara, Kohei Miyazono, Masao Saitoh
  • Organizer: 5^<th> International EMT Meeting
  • Place of Presentation: Biopolis (Singapore)
  • Year and Date: 2011-10-11
• [Presentation] TGF-beta swiches isoform of FGF receptors and regulates EMT of cancer-associated epithelial cells (2011)
  • Author(s): Shirakihara T, Miyazono K, Saitoh M
  • Organizer: 5th International EMT Meeting
  • Place of Presentation: Biopolis (Singapore)