Investigation of transcription factors that regulate development of Plasmodium gametocytes.

• Project/Area Number: 23790458
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Mie University
• Principal Investigator: KANEKO Izumi 三重大学, 大学院・医学系研究科, 助教 (20515720)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: マラリア原虫 / 転写因子 / マラリア

Research Abstract

We identified two transcription factors AP2-G1 and AP2-G2 that regulate sexual development of Plasmodium gametocyte. In addition, we identified a male specific chromatin remodeling complex SWI/SNF subunit that is involved in male gametocyte development. We performed ChIP-seq of these 3 factors and identified their target genes.We analyzed the expression and function of these genes. Our study indicated that these transcription factors and a SWI/SNF subunit regulate gene expression independently or cooperatively during gametocyte development.

Research Products

• [Journal Article] Identification of an AP2-family protein that is critical for malaria liver stage development. (2012)
  • Author(s): Iwanaga S, Kaneko I, Kato T, Yuda M.
  • Journal Title: PLoS One Volume: 7(11)
  • Peer Reviewed
• [Journal Article] Centromere plasmid: a new genetic tool for the study of Plasmodium falciparum (2012)
  • Author(s): Iwanaga S, Kato T, Kaneko I, Yuda M
  • Journal Title: PLoS One Volume: 7(3)
  • Peer Reviewed
• [Journal Article] A high-coverage artificial chromosome library for the genome-wide screening of drug-resistance genes in malaria parasites (2012)
  • Author(s): Iwanaga S, Kaneko I, Yuda M.
  • Journal Title: Genome Res Volume: 22(5) Pages: 985-92
  • Peer Reviewed
• [Journal Article] A transcription factor that is critical for malaria liver stage development (2012)
  • Author(s): Iwanaga S, Kaneko I, Kato T, Yuda M.
  • Journal Title: PLoS One. Volume: 7(11)
  • Peer Reviewed
• [Journal Article] Centromere Plasmid: A New Genetic Tool for the Study of Plasmodium falciparum. (2012)
  • Author(s): Iwanaga S, Kato T, Kaneko I, Yuda M.
  • Journal Title: PLoS One. Volume: 7(3) Pages: 33326-33326
  • Peer Reviewed
• [Presentation] マラリア原虫転写因子AP2-Oのオーキネート形成に果たす役割 (2013)
  • Author(s): 金子伊澄、加藤知美、岩永史朗、油田正夫
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] マラリア原虫転写因子AP2-Oのオーキネート形成に果たす役割 (2013)
  • Author(s): 金子伊澄
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学
• [Presentation] マラリア原虫肝臓ステージの遺伝子発現を制御する転写因子の研究 (2012)
  • Author(s): 金子伊澄、岩永史朗、加藤知美、油田正夫
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学
• [Presentation] マラリア原虫人工染色体を用いた薬剤耐性遺伝子同定法の開発 (2012)
  • Author(s): 岩永史朗、金子伊澄、油田正夫
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学