| Project/Area Number |
23790459
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TOKUNAGA Naohito 愛媛大学, 総合科学研究支援センター, 技術員 (30596151)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | マラリア原虫 / スポロゾイト / ロプトリー |
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| Research Abstract |
To elucidate the molecular mechanisms of Plasmodium sporozoite invasion of the target cells, we attempted to identify rhoptry proteins in sporozoites. Gene expression, protein expression and localization analyses in sporozoites were performed, then 8 novel sporozoite rhoptry molecules were identified. Since these proteins are also expressed in merozoites, development of sporozoite stage-specific gene silencing system is required for functional analysis. To achieve this purpose, screening for suitable candidate promoters to swap was performed. By swapping the original promoter with the candidate promoter using homologous recombination system, we succeeded to generate sporozoite stage-specific gene silencing transgenic parasites. Using this system, it was indicated that one of the rhoptry proteins is involved in sporozoite salivary gland invasion.
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