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Elucidation of the carbohydrate recognization mechanisms by intestinal protozoa using MUC2 glycopeptides

Research Project

Project/Area Number 23790460
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Parasitology (including Sanitary zoology)
Research InstitutionNagasaki University

Principal Investigator

KATO Kentaro  長崎大学, 熱帯医学研究所, 助教 (50508885)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords原虫 / 腸管粘膜糖鎖 / 赤痢アメーバレクチン / 糖鎖認識特異性 / MUC2糖ペプチド / 腸管寄生原虫 / 宿主粘膜糖鎖 / 粘膜糖鎖
Research Abstract

Among intestinal protozoan parasites, Entamoeba histolytica infects large intestine and Cryptosporidium parvum infects small intestine via carbohydrates of the mucus. To elucidate the reason for this difference in the site of infections, recombinant lectins expressed in E. coli and 56 kinds of synthetic glycopeptides were prepared.
However, it was difficult to obtain an active Entamoeba lectin. Therefore, a new assay using Entamoeba histolytica membrane and neo-glycoproteins was established. The reason for mouse strain-dependent susceptibility to Entamoeba histolytica infection was elucidated by using this assay.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (24 results)

All 2013 2012 2011 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (20 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica2013

    • Author(s)
      Kentaro Kato, Yasuhiro Takegawa, Katherine S. Ralston, Carol A. Gilchrist, Shinjiro Hamano, William A. Petri Jr., Yasuro Shinohara
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 436 Issue: 2 Pages: 252-258

    • DOI

      10.1016/j.bbrc.2013.05.085

    • NAID

      120006986202

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl)2013

    • Author(s)
      加藤健太郎
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2013-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 赤痢アメーバ感染における宿主粘膜糖鎖の役割2013

    • Author(s)
      加藤健太郎
    • Organizer
      第11回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎県長崎市
    • Year and Date
      2013-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] シアル酸依存的なEntamoeba histolytica感染感受性2013

    • Author(s)
      加藤健太郎
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京都文京区
    • Year and Date
      2013-03-31
    • Related Report
      2013 Final Research Report
  • [Presentation] 赤痢アメーバ感染における宿主粘膜糖鎖の役割2013

    • Author(s)
      加藤健太郎
    • Organizer
      第11回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎大学(長崎県長崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of a carbohydrate recognition domain of the intermediate subunit of Entamoeba histolytica lectin (Igl)2013

    • Author(s)
      加藤健太郎、Dhoubhadel Bhim G.、藤井仁人、橘裕司
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県神戸市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] シアル酸依存的な Entamoeba histolytica 感染感受性2013

    • Author(s)
      Kentaro Kato, Yasuhiro Takegawa, Katherine S. Ralston, Carol A. Gilchrist, Shinjiro Hamano, William A. Petri Jr., Yasuro Shinohara
    • Organizer
      第82回日本寄生虫学会大会
    • Place of Presentation
      東京 (東京医科歯科大学湯島キャンパス)
    • Related Report
      2012 Research-status Report
  • [Presentation] Mucin glycosylation dependent susceptibilities of three mouse strains against Entamoeba histolytica infection2012

    • Author(s)
      加藤健太郎
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Sialic acid dependent susceptibilities of three mouse strains against Entamoeba histolytica infection2012

    • Author(s)
      加藤健太郎
    • Organizer
      International Symposium of Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
    • Place of Presentation
      東京都文京区
    • Year and Date
      2012-11-30
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス腸管ムチンの糖鎖構造に関する研究2012

    • Author(s)
      加藤健太郎
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫県西宮市
    • Year and Date
      2012-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 赤痢アメーバの感染性がマウス系統間で異なるのは何故?2012

    • Author(s)
      加藤健太郎
    • Organizer
      感染症若手フォーラム
    • Place of Presentation
      長崎県長崎市
    • Year and Date
      2012-02-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Sialic acid dependent susceptibilities of three mouse strains against Entamoeba histolytica infection2012

    • Author(s)
      Kentaro Kato, Yasuhiro Takegawa, Katherine S. Ralston, Carol A. Gilchrist, Shinjiro Hamano, William A. Petri Jr., Yasuro Shinohara
    • Organizer
      International Symposium of Glyco-minded Biology of Diseases as a Basis of Pharmaceutical Sciences
    • Place of Presentation
      Tokyo, Japan (Ito Memorial Hall, The University of Tokyo)
    • Related Report
      2012 Research-status Report
  • [Presentation] Mucin glycosylation dependent susceptibilities of three mouse strains against Entamoeba histolytica infection2012

    • Author(s)
      Kentaro Kato, Yasuhiro Takegawa, Katherine S. Ralston, Carol A. Gilchrist, Shinjiro Hamano, William A. Petri Jr., Yasuro Shinohara
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡 (福岡国際会議場・マリンメッセ福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] 赤痢アメーバの感染性がマウス系統間で異なるのは何故?2012

    • Author(s)
      加藤健太郎
    • Organizer
      感染症若手フォーラム
    • Place of Presentation
      「やすらぎ伊王島」海の見えるホテル(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] マウス腸管ムチンの糖鎖構造に関する研究2012

    • Author(s)
      加藤健太郎、武川泰啓、篠原康郎、濱野真二郎
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大学西宮キャンパス(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] A comparative study on glycosylation patterns of mucus glycoprotein among three mouse strains2011

    • Author(s)
      加藤健太郎
    • Organizer
      第19回分子寄生虫学ワークショップ
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2011-10-23
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス腸管ムチンの糖鎖構造に関する研究2011

    • Author(s)
      加藤健太郎
    • Organizer
      第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
    • Place of Presentation
      東京都港区
    • Year and Date
      2011-07-17
    • Related Report
      2013 Final Research Report
  • [Presentation] A comparative study on glycosylation patterns of mucus glycoproteins among three mouse strains2011

    • Author(s)
      加藤健太郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス腸管ムチンの糖鎖構造に関する研究2011

    • Author(s)
      加藤健太郎、濱野真二郎
    • Organizer
      第80回日本寄生虫学会大会・第22回日本臨床寄生虫学会大会
    • Place of Presentation
      東京都慈恵会医科大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] A comparative study on glycosylation patterns of mucus glycoprotein among three mouse strains2011

    • Author(s)
      加藤健太郎
    • Organizer
      第19回分子寄生虫学ワークショップ
    • Place of Presentation
      神戸市立神戸セミナーハウス(兵庫県)
    • Related Report
      2011 Research-status Report
  • [Presentation] A comparative study on glycosylation patterns of mucus glycoproteins among three mouse strains2011

    • Author(s)
      加藤健太郎、武川泰啓、濱野真二郎、篠原康郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Book] 感染症事典2012

    • Author(s)
      加藤健太郎, 他
    • Publisher
      オーム出版
    • Related Report
      2013 Final Research Report
  • [Book] 感染症事典2012

    • Author(s)
      感染症事典編集委員会(共著)
    • Total Pages
      619
    • Publisher
      オーム社
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://research.jimu.nagasaki-u.ac.jp/IST?ISTActId=FINDJPDetail&ISTKidoKbn=&ISTErrorChkKbn=&ISTFormSetKbn=&ISTTokenChkKbn=&userId=100000518

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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