Research on geographical origin and spread of drug resistant Plasmodium vivax in Asia

• Project/Area Number: 23790465
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: IWAGAMI Moritoshi 独立行政法人国立国際医療研究センター, 熱帯医学マラリア研究部, 上級研究員 (20392392)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 三日熱マラリア原虫 / 薬剤耐性 / 分子疫学研究 / クロロキン / アジア

Research Abstract

Genetic polymorphisms of chloroquine (CQ) resistant gene and the polymorphisms flanking the gene of Plasmodium vivax in Asia were examined. All the isolates from the Philippines where treatment failure to CQ had never been reported possessed resistant genotype. On the other hand, all the isolates from the Republic of Korea where treatment failure to CQ had been reported possessed susceptible genotype. These results suggested that the polymorphisms of the resistant gene were local genotypes and that the association between the genotypes and CQ susceptibility were elusive.

Research Products

• [Journal Article] First case of Plasmodium knowlesi infection in a Japanese traveller returning from Malaysia (2013)
  • Author(s): Ryutaro Tanizaki, Mugen Ujiie, Yasuyuki Kato, Moritoshi Iwagami, Aki Hashimoto, Satoshi Kutsuna, Nozomi Takeshita, Kyoko Hayakawa, Shuzo Kanagawa, Shigeyuki Kano, Norio Ohmagari.
  • Journal Title: Malaria Journal Volume: 12 Issue: 1 Pages: 128-128
  • DOI: 10.1186/1475-2875-12-128
  • Peer Reviewed
• [Journal Article] マラリア診断法3種(顕微鏡検査、迅速診断キット、PCR 法)の有用性 : 結果が一致しなかった症例の分析 (2012)
  • Author(s): 石上盛敏、駒木-安田加奈子、氏家無限、竹下望、加藤康幸、金川修造、大曲貴夫、狩野繁之
  • Journal Title: 日本臨床寄生虫学会誌 Volume: 23巻 Pages: 77-81
  • Peer Reviewed
• [Journal Article] Population structure and transmission dynamics of Plasmodium vivax in the Republic of Korea based on microsatellite DNA analysis. (2012)
  • Author(s): Iwagami M, Fukumoto M, Hwang SY, Kim SH、Kho WG, Kano S
  • Journal Title: PLoS Neglected Tropical Diseases Volume: Vol.6 Issue: 4 Pages: e1592-e1592
  • DOI: 10.1371/journal.pntd.0001592
  • Peer Reviewed
• [Journal Article] Morphology and surface topography of the schistosome Bivitellobilharzia nairi from the Asian elephant (Elephas maximus maximus) in Sri Lanka. (2012)
  • Author(s): Jayanthe RPV. Rajapakse, Moritoshi Iwagami, S. Wickramasinghe, SM.Walker,Takeshi Agatsuma
  • Journal Title: Journal of Helminthology Volume: 86 Issue: 3 Pages: 1-8
  • DOI: 10.1017/s0022149x12000417
  • Peer Reviewed
• [Journal Article] マラリア診断法３種（顕微鏡検査、迅速診断キット、PCR法）の有用性：結果が一致しなかった症例の分析 (2012)
  • Author(s): 石上盛敏, 駒木-安田加奈子, 氏家無限, 竹下望, 加藤康幸, 金川修造, 大曲貴夫, 狩野繁之
  • Journal Title: 日本臨床寄生虫学会誌 Volume: 23 Pages: 77-81
  • Peer Reviewed
• [Presentation] Polymorphisms of pvmdr1 and microsatellite DNA of Plasmodium vivax population showed no evidence of selective sweep by chloroquine pressure (2013)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学, 東京
• [Presentation] Polymorphisms of pvmdr1 and microsatellite DNA of Plasmodium vivax population showed no evidence of selective sweep by chloroquine pressure (2013)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学、 東京都
• [Presentation] わが国初の輸入P. knowlesiマラリアのDNA塩基配列レベルでの確定診断 (2013)
  • Author(s): 狩野繁之, 石上盛敏, 駒木-安田加奈子, 矢野和彦, 狩野俊吾, 谷崎隆太郎,氏家無限, 加藤康幸, 怱那賢志, 竹下望, 早川佳代子, 金川修造, 大曲貴夫
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学、 東京都
• [Presentation] マラリア診断法3種(顕微鏡検査、迅速診断キット、PCR 法)の有用性:結果が一致しなかった症例の分析 (2012)
  • Author(s): 石上盛敏、駒木-安田加奈子、氏家無限、竹下望、加藤康幸、金川修造、大曲貴夫、狩野繁之
  • Organizer: 第23回日本臨床寄生虫学会大会
  • Place of Presentation: 東京女子医科大学、東京都
  • Year and Date: 2012-06-23
• [Presentation] Analysis of pvmdr1 and microsatellite DNA loci flanking the gene of Plasmodium vivax collected worldwide showed no evidence of chloroquine pressure (2012)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: Joint International Tropical Medicine Meeting 2012 & The 7th Seminar on Food- and Water-Borne Parasitic Zoonoses
  • Place of Presentation: Centara Grand & Bangkok Convention Centre, Bangkok, Thailand
• [Presentation] Evidence of selective sweep by chloroquine pressure was not detected in codon 976 of pvmdr1 and microsatellite DNA loci flanking the gene of Plasmodium vivax isolates collected worldwide (2012)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第72回日本寄生虫学会東日本支部大会・第10回分子寄生虫・マラリアフォーラム合同大会
  • Place of Presentation: 群馬大学医学部, 前橋市
• [Presentation] Molecular genetic epidemiology on pvmdr1 mutation of Plasmodium vivax (2012)
  • Author(s): Iwagami M, Rivera PT, Villacorte EA, Kho WG, Kano S
  • Organizer: The XVIII International Congress for Tropical Medicine and Malaria and the XLVIII Congress for the Brazilian Society of Tropical Medicine, Royal Tulip Rio de Janeiro
  • Place of Presentation: Rio de Janeiro, Brazil
• [Presentation] Molecular epidemiology on chloroquine resistant gene of Plasmodium vivax in the Republic of Korea, the Philippines and other endemic countries based on pvmdr1 genotype and microsatellite DNA polymorphisms flanking the gene (2012)
  • Author(s): Iwagami M, Kho WG, Kim SH, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ、帯広市
• [Presentation] Molecular genetic epidemiology on pvmdr1 mutation of Plasmodium vivax in the Republic of Korea and other endemic areas (2012)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: Forum Cheju 15, The 15th Japan-Korea Parasitologists ' Seminar
  • Place of Presentation: Miyazaki Aoshima Palm Beach Hotel, Miyazaki, Japan
• [Presentation] Molecular epidemiology on pvmdr1 genotype of Plasmodium vivax in the Philippines and the other endemic areas (2012)
  • Author(s): Iwagami M, Rivera PT, Villacorte EA, Kho WG, Kano S
  • Organizer: 5^<th> ASEAN Congress of Tropical Medicine and Parasitology
  • Place of Presentation: University of the Philippines Manila, Manila, The Philippines
• [Presentation] 三日熱マラリア原虫pvmdr1 変異に関する遺伝疫学 (2012)
  • Author(s): 石上盛敏、Weon-Gyu Kho 、Pilarita T Rivera、Elena A Villacorte、狩野繁之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学西宮キャンパス
• [Presentation] Molecular genetic epidemiology on pvmdr1 mutation of Plasmodium vivax (2012)
  • Author(s): Iwagami M, Rivera PT, Villacorte EA, Kho WG, Kano S
  • Organizer: The XVIII International Congress for Tropical Medicine and Malaria and the XLVIII Congress for the Brazilian Society of Tropical Medicine
  • Place of Presentation: Royal Tulip Rio de Janeiro, Rio de Janeiro, Brazil
• [Presentation] Molecular genetic epidemiology on pvmdr1 mutation of Plasmodium vivax in the Republic of Korea and other endemic areas (2012)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: Forum Cheju 15, The 15th Japan-Korea Parasitologists’ Seminar
  • Place of Presentation: Miyazaki Aoshima Palm Beach Hotel, Miyazaki, Japan
• [Presentation] Molecular epidemiology on pvmdr1 genotype of Plasmodium vivax in the Philippines and the other endemic areas (2012)
  • Author(s): Iwagami M, Rivera PT, Villacorte EA, Kho WG, Kano S
  • Organizer: 5th ASEAN Congress of Tropical Medicine and Parasitology
  • Place of Presentation: University of the Phil-ippines Manila, Manila, The Philippines
• [Presentation] Evidence of selective sweep by chloroquine pressure was not detected in codon 976 of pvmdr1 and microsatellite DNA loci flanking the gene of Plasmodium vivax isolates collected worldwide (2012)
  • Author(s): Iwagami M, Kho WG, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第72回日本寄生虫学会東日本支部大会・第10回分子寄生虫・マラリアフォーラム合同大会
  • Place of Presentation: 群馬大学医学部、前橋市
• [Presentation] Molecular epidemiology on chloroquine resistant gene of Plasmodium vivax in the Republic of Korea, the Philippines and other endemic countries based on pvmdr1 genotype and microsatellite DNA polymorphisms flanking the gene (2012)
  • Author(s): Iwagami M, Kho WG, Kim SH, Rivera PT, Villacorte EA, Kano S
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: とかちプラザ、帯広市
• [Presentation] 渡航先でスタンドバイ治療を行い、帰国後に熱帯熱マラリアであったと考えられた1例 (2012)
  • Author(s): 日高秀信, 渡邊浩, 石上盛敏, 狩野繁之
  • Organizer: 第16回日本渡航医学会学術集会
  • Place of Presentation: 大阪大学、吹田市
• [Presentation] マラリア診断法３種（顕微鏡検査、迅速診断キット、PCR法）の有用性：結果が一致しなかった症例の分析 (2012)
  • Author(s): 石上盛敏, 駒木-安田加奈子, 氏家無限, 竹下望, 加藤康幸, 金川修造, 大曲貴夫, 狩野繁之
  • Organizer: 第23回日本臨床寄生虫学会大会
  • Place of Presentation: 東京女子医科大学、 東京都
• [Presentation] 三日熱マラリア原虫pvmdr1 変異の分布に関する分子疫学研究 (2011)
  • Author(s): 石上盛敏、Weon-Gyu Kho 、Pilarita T Rivera、Elena A Villacorte、狩野繁之
  • Organizer: 第71回日本寄生虫学会東日本支部大会
  • Place of Presentation: 杏林大学医学部、東京都
  • Year and Date: 2011-10-01
• [Presentation] Pvmdr1 mutation for genetic epidemiology of chloroquine resistant Plasmodium vivax (2011)
  • Author(s): Iwagami M, Rivera PT, Villacorte EA, Kho WG, Kano S
  • Organizer: American Society of Tropical Medicine and Hygiene 60th Annual Meeting
  • Place of Presentation: Philadelphia Marriott Downtown Hotel, Philadelphia, PA, USA
• [Presentation] 三日熱マラリア原虫pvmdr1変異の分布に関する分子疫学研究
  • Author(s): 石上盛敏、Weon-Gyu Kho、Pilarita T Rivera、Elena A Villacorte、狩野繁之
  • Organizer: 第71回日本寄生虫学会東日本支部大会
  • Place of Presentation: 杏林大学医学部
• [Presentation] 韓国の三日熱マラリア原虫のマイクロサテライトDNA多型に基づく分子遺伝疫学
  • Author(s): 石上盛敏、福本恵、Weon-Gyu Kho、狩野繁之
  • Organizer: 第52回日本熱帯医学会大会・第26回日本国際保健医療学会学術大会
  • Place of Presentation: 東京大学本郷キャンパス
• [Presentation] Pvmdr1 mutation for genetic epidemiology of chloroquine resistant Plasmodium vivax
  • Author(s): Moritoshi Iwagami、Pilarita T Rivera、Elena A Villacorte、Weon-Gyu Kho、Shigeyuki Kano
  • Organizer: American Society of Tropical Medicine and Hygiene 60th Annual Meeting
  • Place of Presentation: Philadelphia Marriott Downtown Hotel, Philadelphia, PA, USA
• [Presentation] 三日熱マラリア原虫pvmdr1変異に関する遺伝疫学
  • Author(s): 石上盛敏、Weon-Gyu Kho、Pilarita T Rivera、Elena A Villacorte、狩野繁之
  • Organizer: 第81回日本寄生虫学会大会
  • Place of Presentation: 兵庫医科大学西宮キャンパス
• [Remarks]
  • URL: http://www.rincgm.jp/department/lab/01/