Investigation of the role of antioxidant protein PrxI against tuberculosis

• Project/Area Number: 23790466
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: OKUMURA Kayo 帯広畜産大学, 畜産学部, 助教 (70415561)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 細菌 / 感染症 / 結核 / マクロファージ

Research Abstract

To determine whether PrxI is essential for the host defense against tuberculosis, susceptibility of PrxI-deficient (PrxI KO) mice to Mycobacterium tuberculosis (Mtb) was compared to wild-type mice in vivo. We observed that PrxI KO mouse infected that with virulent Mtb survived significantly shorter as compared to the wild-type controls. Also we found that PrxI bound to Mtb specific protein.

Research Products

• [Journal Article] Complete Genome Sequence of Melissococcus plutonius DAT561, a Strain That Shows an Unusual Growth Profile and Is Representative of an Endemic Cluster in Japan (2012)
  • Author(s): Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
  • Journal Title: J Bacteriol Volume: 194(11) Issue: 11 Pages: 3014-3014
  • DOI: 10.1128/jb.00437-12
  • Peer Reviewed
• [Journal Article] Evolutionary paths of streptococcal and staphylococcal superantigens. (2012)
  • Author(s): Okumura K, Shimomura Y, Yamagata Murayama S, Yagi J, Ubukata K, Kirikae T, Miyoshi-Akiyama T.
  • Journal Title: BMC Genomics Volume: 13 Issue: 1 Pages: 404-404
  • DOI: 10.1186/1471-2164-13-404
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Melissococcus plutonius ATCC 35311 (2011)
  • Author(s): Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
  • Journal Title: J Bacteriol Volume: 193(15) Issue: 15 Pages: 4029-30
  • DOI: 10.1128/jb.05151-11
  • Peer Reviewed
• [Journal Article] Complete genome sequence of Melissococcus plutonius ATCC 35311. (2011)
  • Author(s): Okumura K, Arai R, Okura M, Kirikae T, Takamatsu D, Osaki M, Miyoshi-Akiyama T.
  • Journal Title: Journal of Bacteriology Volume: 193 Pages: 4029-4030
  • Peer Reviewed
• [Presentation] A mutation in the decoding region of 16S rRNA attenuates the virulence of Mycobacterium tuberculosis (2013)
  • Author(s): 渡邊真弥、祝弘樹、船渡川圭次、はい島由二、奥村香世、加藤雅子、橋本雅仁、切替富美子、秋山徹、切替照雄
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2013-03-18
• [Presentation] Mycobacterial PE_PGRS62 is a virulence factor during tuberculosis (2013)
  • Author(s): 祝弘樹、船渡川圭次、渡邊真弥、奥村香世、はい島由二、加藤雅子、切替富美子、秋山徹、切替照雄
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2013-03-18
• [Presentation] Melissococcus plutonius 典型株・非典型株識別用Duplex PCR法の開発 (2013)
  • Author(s): 高松大輔、大倉正稔、奥村香世、秋山徹
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2013-03-18
• [Presentation] A mutation in the decoding region of 16S rRNA attenuates the virulence of Mycobacterium tuberculosis (2013)
  • Author(s): 渡邊真弥、祝弘樹、船渡川圭次、はい島由二、奥村香世、加藤雅子、橋本雅仁、切替富美子、秋山徹、切替照雄
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] Mycobacterial PE_PGRS62 is a virulence factor during tuberculosis (2013)
  • Author(s): 祝弘樹、船渡川圭次、渡邊真弥、奥村香世、はい島由二、加藤雅子、切替富美子、秋山徹、切替照雄
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] Melissococcus plutonius典型株・非典型株識別用Duplex PCR法の開発 (2013)
  • Author(s): 高松大輔、大倉正稔、奥村香世、秋山徹
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 幕張メッセ（千葉県）
• [Presentation] レンサ球菌および黄色ブドウ球菌のスーパー抗原の起源に関する解析 (2012)
  • Author(s): 奥村香世、下村有美、村山〓明、八木淳二、切替照雄、秋山徹
  • Organizer: 日本細菌学会北海道支部学術総会
  • Place of Presentation: とかちプラザ
  • Year and Date: 2012-08-29
• [Presentation] Complete genome sequence of Melissococcus plutonius ATCC 35311. (2011)
  • Author(s): K. Okumura, R. Arai, M. Okura, T. Kirikae, D. Takamatsu, M. Osaki, T. Miyoshi-Akiyama.
  • Organizer: 111th General Meeting American Society for Microbiology
  • Place of Presentation: New Orleans, USA
• [Presentation] 日本におけるヨーロッパ腐蛆病菌（Melissococcus plutonius）株の多様性とゲノム解析によるカリウム要求性に関与する遺伝子の同定. (2011)
  • Author(s): 荒井理恵, 奥村香世, 富永潔, 秋山徹, 大倉正稔, 切替照雄, 伊藤一智, 岡村直美, 大西英高, 大崎慎人, 高松大輔.
  • Organizer: 第20回 Lancefieldレンサ球菌研究会
  • Place of Presentation: 愛知学院大学, 愛知県
• [Presentation] Complete Genome Sequence of European foulbrood pathogen Melissococcus plutonius ATCC 35311. (2011)
  • Author(s): K. Okumura, R. Arai, M. Okura, T. Kirikae, D. Takamatsu, M. Osaki, T. Miyoshi-Akiyama.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center, Sapporo
• [Presentation] レンサ球菌および黄色ブドウ球菌のスーパー抗原の起源に関する解析
  • Author(s): 奥村香世、下村有美、村山琮明、八木淳二、切替照雄、秋山徹
  • Organizer: 日本細菌学会北海道支部学術総会
  • Place of Presentation: とかちプラザ（北海道）
• [Presentation] Melissococcus plutonius典型株・非典型株識別用Duplex PCR法の開発
  • Author(s): 荒井理恵、奥村香世、秋山徹、森永結子、呉梅花、芳山三喜雄、大倉正稔、高松大輔
  • Organizer: 日本獣医学会学術集会
  • Place of Presentation: 東京大学（東京都）