Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Research Abstract |
Effect of toxic shock syndrome toxin-1 (TSST-1) on Staphylococcus aureus infection in the epithelial cells was investigated. TSST-1 did not alter the adhesion and invasion into the epithelial cells, HeLa 229, HEK293 and 407. However, TSST-1 reduced the accumulation of autophagosomes in nutrient starved cells or rapamycin-treated cells, suggesting that TSST-1 suppresses autophagy. In addition, this suppression did not require superantigenic activity and might be involved in the autophagosome formation process rather than autophagosome degradation. From day 5 after infection, intracellular bacterial number and cytotoxicity of TSST-1-producing S. aureus were deceased in comparison to non-producing strain, suggesting that TSST-1 may promote intracellular persistence of S. aureus in the epithelial cells.
|