Molecular analysis of Shigella effector IpaH E3 ligase activity
Project/Area Number |
23790472
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bacteriology (including Mycology)
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Research Institution | The University of Tokyo |
Principal Investigator |
ASHIDA Hiroshi 東京大学, 医科学研究所, 特任助教 (10535115)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | エフェクター / 赤痢菌 / ユビキチン / NF-〓B / シグナル伝達 / 感染 / 炎症 |
Research Abstract |
In this study, we tried to identify the mechanism of IpaH0722 in Shigella infection. IpaH0722, which has an E3 ubiquitin ligase activity, inhibits NF-〓B activity in its E3 ubiquitin ligase dependent manner. As a result, we found that IpaH0722 inhibits phagosome disruption mediated PKC-NF-〓B activation by targeting TRAF2 for ubiquitination and proteasome-dependent degradation, thereby downregulating host inflammatory responses.
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Report
(3 results)
Research Products
(24 results)
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[Journal Article] The Shigella flexneri effector OspI deamidates UBC13 to dampen the inflammatory response.2012
Author(s)
Sanada T, Kim M, Mimuro H, Suzuki M, Ogawa M, Oyama A, Ashida H, Kobyashi T, Koyama T, Nagai S, Shibata Y, Gohda J, Inoue J, Mizushima T, & Sasakawa C.
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Journal Title
Nature.
Volume: 483
Pages: 623-626
Related Report
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[Journal Article] A bacterial effector deamidates Ubc13 to dampen the inflammatory response2012
Author(s)
Sanada, T., Kim, M., Mimuro, H., Suzuki, M., Ogawa, M., Oyama, A., Ashida, H., Kobyashi, T., Koyama, T., Nagai, S., Shibata, Y., Gohda, J., Inoue, J., Mizushima, T., and Sasakawa, C
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Journal Title
Nature
Volume: 483
Pages: 623-626
Related Report
Peer Reviewed
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[Journal Article] A tecpr1-dependent selective autophagy pathway targets bacterial pathogens.2011
Author(s)
Ogawa M, Yoshikawa Y, Kobayashi T, Mimuro H, Fukumatsu M, Kiga K, Piao Z, Ashida H, Yoshida M, Kakuta S, Koyama T, Goto Y, Nagatake T, Nagai S, Kiyono H, Kawalec M, Reichhart JM, Sasakawa C.
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Journal Title
Cell Host Microbe.
Volume: 9
Pages: 376-89
Related Report
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[Journal Article] Cell death and infection: a double-edged sword for host and pathogen survival2011
Author(s)
Ashida, H., Mimuro, H., Ogawa, M., Kobayashi, T., Sanada, T., Kim, M., and Sasakawa, C
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Journal Title
Journal of Cell BIology
Volume: 195
Pages: 931-942
Related Report
Peer Reviewed
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[Journal Article] Shigella are versatile mucosal pathogens that circumvent the host innate immune system2011
Author(s)
Ashida, H., Ogawa, M., Mimuro, H., Kobayashi, T., Sanada, T., and Sasakawa, C
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Journal Title
Current Opinion in Immunology
Volume: 23
Pages: 448-455
Related Report
Peer Reviewed
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[Journal Article] A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens2011
Author(s)
Ogawa, M., Yoshikawa, Y., Kobayashi, T., Mimuro, H., Fukumatsu, M., Kiga, K., Piao, Z., Ashida, H.,....., Kawalec, M., Reichhart, J. M., and Sasakawa, C
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Journal Title
Cell Host & Microbe
Volume: 9
Pages: 376-389
Related Report
Peer Reviewed
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[Journal Article] BabA-mediated adherence is a potentiator of the Helicobacter pylori type IV secretion system activity.2011
Author(s)
Ishijima, N., Suzuki, M., Ashida, H., Ichikawa, Y., Kanegae, Y., Saito, I., Borén, T., Haas, R., Sasakawa, C., and Mimuro, H
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Journal Title
Journal of Biological Chemistry
Volume: 286
Pages: 25256-23264
Related Report
Peer Reviewed
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