Analysis of qualitative and quantitative secretory regulation of Vibrio parahaemolyticusT3SS2

• Project/Area Number: 23790474
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: KODAMA Toshio 大阪大学, 微生物病研究所, 助教 (20346133)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 腸炎ビブリオ / 3型分泌装置 / エフェクター / Vibrio parahaemolyticus / T3SS / effector / enterotoxicity

Research Abstract

Type III secretion system 2 of Vibrio parahaemolyticus is essential for entrotoxicity. However, qualitative and quantitative regulation of T3SS2 is unclear. In this study, I identified two novel genes responsible for secretory regulation of T3SS2. The knock-out strains of these genes caused a dramatic change in a secretory pattern of T3SS2 secreted proteins, indicating that these genes are involved in qualitative and quantitative secretory regulation of Vibrio parahaemolyticus T3SS2.

Research Products

• [Journal Article] Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae (2013)
  • Author(s): Morita M, Yamamoto S, Hiyoshi H, Kodama T,Okura M, Arakawa E, Alam M, Ohnish M,Izumiya H, Watanabe H
  • Journal Title: Microbiol Immunol Volume: 57(5) Issue: 5 Pages: 334-9
  • DOI: 10.1111/1348-0421.12039
  • Peer Reviewed
• [Journal Article] Vibrio parahaemolyticus effector proteins suppress inflammasome activation by interfering with host autophagy signaling (2013)
  • Author(s): Naomi Higa, Claudia Toma, Yukiko Koizumi, Noboru Nakasone, Toshitsugu Nohara and Toshihiko Suzuki
  • Journal Title: PloS Pathogen Volume: 9(1) Issue: 1 Pages: e1003142-e1003142
  • DOI: 10.1371/journal.ppat.1003142
  • Peer Reviewed
• [Journal Article] Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae. (2013)
  • Author(s): Morita M, Yamamoto S, Hiyoshi H, Kodama T, Okura M, Arakawa E, Alam M, Ohnishi M, Izumiya H, Watanabe H.
  • Journal Title: Microbiol Immunol. Volume: Epub ahead of print Pages: n/a-n/a
  • DOI: 10.1111/j.1348-0421.12039
  • Peer Reviewed
• [Journal Article] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in Escherichia coli K-12 strain as a molecular syringe. (2012)
  • Author(s): Akeda Y, Kimura T, Yamasaki A, Kodama T, Iida T, Honda T, Oishi K.
  • Journal Title: Biochem Biophys Res Commun. Volume: 427(2) Issue: 2 Pages: 242-7
  • DOI: 10.1016/j.bbrc.2012.09.018
  • Peer Reviewed
• [Journal Article] A Cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes. (2012)
  • Author(s): Matsuda S, Okada N, Kodama T, Honda T, Iida T.
  • Journal Title: PLoS pathogens. Volume: 8(7) Issue: 7 Pages: e1002803-e1002803
  • DOI: 10.1371/journal.ppat.1002803
  • Peer Reviewed
• [Journal Article] The hydrophilic translocator for Vibrio parahaemolyticus T3SS2 is also translocated. (2012)
  • Author(s): Zhou X, Ritchie MJ, Hiyoshi H, Iida T, Davis NB, Waldor KM, Kodama T.
  • Journal Title: Infect Immun. Volume: 80(8) Issue: 8 Pages: 2940-7
  • DOI: 10.1128/iai.00402-12
  • Peer Reviewed
• [Journal Article] Vibrio parahaemolyticus infection (2012)
  • Author(s): Kodama T
  • Journal Title: Antibiotics and Chemotherapy Volume: 28(6) Pages: 63-70
  • URL: https://www.iyaku-j.com/iyakuj/system/M2-1/summary_viewer.php?trgid=24993
• [Journal Article] Hemolysin E- and enterohemolysin-derived hemolytic activity of O55/O157 strains and other Escherichia coli lineages (2012)
  • Author(s): K.Murase, et al
  • Journal Title: Microbiology Volume: 158 Issue: 3 Pages: 746-758
  • DOI: 10.1099/mic.0.054775-0
  • Peer Reviewed
• [Journal Article] Inflammation and Disintegration of Intestinal Villi in an Experimental Model for Vibrio parahaemolyticus-Induced Diarrhea (2011)
  • Author(s): Ritchie JM, Rui H, Zhou X, Iida T, Kodoma T, et al
  • Journal Title: PLoS Patnogens Volume: 8(3) Issue: 3 Pages: e1002593-e1002593
  • DOI: 10.1371/journal.ppat.1002593
  • Peer Reviewed
• [Journal Article] an F-Actin-Binding Type III secretion effector, is Responsible for Vibrio parahaemolyticus-Induced enterotoxicity (2011)
  • Author(s): Hiyoshi H, Kodama T*, Saito K, Gotoh K, Matsuda S, Akeda Y, Honda T, and Iida T. VopV
  • Journal Title: Cell Host Microbe Volume: 4 Pages: 401-19
  • URL: http://dx.doi.org/10.1016/j.chom.2011.08.014
  • Peer Reviewed
• [Journal Article] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-Specific Effector VopC (2011)
  • Author(s): Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
  • Journal Title: FEMS Microbiology Letters Volume: 324 Issue: 2 Pages: 156-164
  • DOI: 10.1111/j.1574-6968.2011.02399.x
  • Peer Reviewed
• [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to β4-integrin expressing cell lines (2011)
  • Author(s): Abolghait SK, Iida T, Kodama T, Cantar elli VV, Akeda Y, Honda T
  • Journal Title: Microb Pathog Volume: 51 Pages: 454-465
  • Peer Reviewed
• [Journal Article] Relationship between Localization on Cellular Membranes and Cytotoxicity of Vibrio vulnificus Hemolysin (2011)
  • Author(s): Sugiyama H, Kashimoto T, Ueno S, Ehara H, Kodama T, Iida T, Susa N
  • Journal Title: PLoS One Volume: 6(10) Issue: 10 Pages: e26018-e26018
  • DOI: 10.1371/journal.pone.0026018
  • Peer Reviewed
• [Journal Article] Haemolysin E- and enterohaemolysin-derived haemolytic activity of O55/O157 strains and other Escherichia coli lineages (2011)
  • Author(s): Murase K, Ooka T, Iguchi A, Ogura Y, Nakayama K, Asadulghani M, Islam MR, Hiyoshi H, Kodama T, Beutin L, Hayashi T.
  • Journal Title: Microbiology Volume: 158(Pt3) Pages: 746-758
  • Peer Reviewed
• [Journal Article] VopV, an F-Actin-Binding Type III Secretion Effector, Is Required for Vibrio parahaemolyticus-Induced Enterotoxicity (2011)
  • Author(s): Hirotaka Hiyoshi, Toshio Kodama, Kazunobu Saito, Kazuyoshi Gotoh, Shigeaki Matsuda, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
  • Journal Title: Cell Host & Microbe Volume: 10 Issue: 4 Pages: 401-409
  • DOI: 10.1016/j.chom.2011.08.014
  • Peer Reviewed
• [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to・4-integrin expressing cell lines (2011)
  • Author(s): Said Kamal Abolghait, Tetsuya Iida, Toshio Kodama, Vlademir V. Cantarelli, Yukihiro Akeda and Takeshi Honda
  • Journal Title: Microbial Pathogenesis Volume: 51 Issue: 6 Pages: 454-465
  • DOI: 10.1016/j.micpath.2011.09.006
  • Peer Reviewed
• [Presentation] VepA, a cytotoxic type III effector of Vibrio parahaemolyticus, targets V-ATPase subunit c (2013)
  • Author(s): Shigeaki Matsuda
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場(千葉県)
• [Presentation] VopO, a T3SS2 effector of Vibrio, reorganizes actin cytoskeleton via activation of RhoA-ROCK pathway (2013)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場(千葉県)
• [Presentation] VopC, a Vibrio parahaemolyticus T3SS2 effector, mediates invasion and actin stress fiber formation (2013)
  • Author(s): Ryu Okada
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場(千葉県)
• [Presentation] Mechanism of Vibrio parahaemolyticus-induced enterotoxicity (2013)
  • Author(s): Toshio Kodama
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場(千葉県)
• [Presentation] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in E. coli as a tool (2013)
  • Author(s): Yukihiro Akeda
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場(千葉県)
• [Presentation] VepA, a cytotoxic type III effector of Vibrio parahaemolyticus, targets V-ATPase subunit c (2013)
  • Author(s): Shigeaki Matsuda
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場（千葉県）
• [Presentation] VopO, a T3SS2 effector of Vibrio, reorganizes actin cytoskeleton via activation of RhoA-ROCK pathway (2013)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場（千葉県）
• [Presentation] VopC, a Vibrio parahaemolyticus T3SS2 effector, mediates invasion and actin stress fiber formation (2013)
  • Author(s): Ryu Okada
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場（千葉県）
• [Presentation] Mechanism of Vibrio parahaemolyticus-induced enterotoxicity (2013)
  • Author(s): Toshio Kodama
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場（千葉県）
  • Invited
• [Presentation] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in E. coli as a tool (2013)
  • Author(s): Yukihiro Akeda
  • Organizer: 日本細菌学会 総会
  • Place of Presentation: 幕張メッセ 国際会議場（千葉県）
• [Presentation] Relationship between localization and cytotoxicity of Vibrio vulnificus hemolysin (2012)
  • Author(s): 柏本孝茂
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎県)
  • Year and Date: 2012-03-27
• [Presentation] 新規F-アクチン結合T3SSエフェクターVopVは腸炎ビブリオの下痢原性に寄与する (2012)
  • Author(s): 日吉大貴
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎県)
  • Year and Date: 2012-03-27
• [Presentation] A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H-ATPase subunit c and ruptures host cell lysosomes (2012)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: US-Japan cooperative medical science program
  • Place of Presentation: 千葉大学(千葉県)
• [Presentation] 腸炎ビブリオ3型分泌装置1エフェクターVepAによる細胞毒性の解析 (2012)
  • Author(s): 松田重輝
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 日本文理大学 湯布院研修所(大分県)
• [Presentation] VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity (2012)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: Awaji international forum on infection and immunity
  • Place of Presentation: Awaji Yumebutai international conference center (Hyogo)
• [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターVopVの解析 (2012)
  • Author(s): 日吉大貴
  • Organizer: 毒素シンポジウム
  • Place of Presentation: とかちプラザ(北海道)
• [Presentation] Vibrio vulnificus hemolysin associates with detergent-resistant membranes, but this localization is not essential for its cytotoxicity (2012)
  • Author(s): Takashige Kashimoto
  • Organizer: ASM general meeting
  • Place of Presentation: San Francisco (USA)
• [Presentation] An novel F-actin-binding type III secretion effector is required for Vibrio parahaemolyticus-induced enterotoxicity (2012)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: ASM general meeting
  • Place of Presentation: San Francisco (USA)
• [Presentation] 腸炎ビブリオ３型分泌装置1エフェクターVepAによる細胞毒性の解析 (2012)
  • Author(s): 松田重輝
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 日本文理大学 湯布院研修所（大分県）
• [Presentation] A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H-ATPase subunit c and ruptures host cell lysosomes (2012)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: US-Japan cooperative medical science program
  • Place of Presentation: 千葉大学（千葉県）
• [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターVopVの解析 (2012)
  • Author(s): 日吉大貴
  • Organizer: 毒素シンポジウム
  • Place of Presentation: とかちプラザ（北海道）
• [Presentation] Relationship between localization and cytotoxicity of Vibrio vulnificus hemolysin (2012)
  • Author(s): 柏本孝茂、杉山寛幸、上野俊治、江原勇登、児玉年央、飯田哲也、諏佐信行
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール（長崎県）
• [Presentation] 新規F-アクチン結合T3SSエフェクターVopVは腸炎ビブリオの下痢原性に寄与する (2012)
  • Author(s): 日吉大貴、児玉年央、斉藤和伸、後藤和義、松田重輝、明田幸宏、本田武司、飯田哲也
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール（長崎県）
• [Presentation] A novel F-actin-binding T3SS effector is responsible for Vibrio parahaemolyticus- induced diarrhea (2011)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: US-Japan cooperative medical science program
  • Place of Presentation: National Institute of Cholera and Enteric Diseases (India)
  • Year and Date: 2011-12-15
• [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターの解析 (2011)
  • Author(s): 日吉大貴
  • Organizer: 日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス(大阪府)
  • Year and Date: 2011-11-19
• [Presentation] Hemolysin E- and Enterohemolysin-derived hemolytic activities of the O55/0157 and other Escherichia coli lineages (2011)
  • Author(s): Kazunori Murase
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-07
• [Presentation] A novel F-actin binding T3SS effector is responsible for Vibrio parahaemolyticus-induced diarrhea (2011)
  • Author(s): Hirotaka Hiyoshi
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-09-07
• [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターの解析 (2011)
  • Author(s): 日吉大貴、児玉年央、斉藤和伸、後藤和義、松田重輝、明田幸宏、本田武司、飯田哲也
  • Organizer: 日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス（大阪府）
• [Presentation] Hemolysin E- and Enterohemolysin-derived hemolytic activities of the O55/0157 and other Escherichia coli lineages (2011)
  • Author(s): Kazunori Murase, Tadasuke Ooka, Yoshitoshi Ogura, Atsushi Iguchi, Keisuke Nakayama, Md Asadulghani, Md Rakibul Islam, Hirotaka Hiyoshi, Toshio Kodama, Lothar Beutin, Tetsuya Hayashi
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
• [Presentation] A novel F-actin binding T3SS effector is responsible for Vibrio parahaemolyticus-induced diarrhea (2011)
  • Author(s): Hirotaka Hiyoshi, Toshio Kodama, Takeshi Honda, Tetsuya Iida
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 札幌コンベンションセンター（北海道）