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Analysis of qualitative and quantitative secretory regulation of Vibrio parahaemolyticusT3SS2

Research Project

Project/Area Number 23790474
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

KODAMA Toshio  大阪大学, 微生物病研究所, 助教 (20346133)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords腸炎ビブリオ / 3型分泌装置 / エフェクター / Vibrio parahaemolyticus / T3SS / effector / enterotoxicity
Research Abstract

Type III secretion system 2 of Vibrio parahaemolyticus is essential for entrotoxicity. However, qualitative and quantitative regulation of T3SS2 is unclear. In this study, I identified two novel genes responsible for secretory regulation of T3SS2. The knock-out strains of these genes caused a dramatic change in a secretory pattern of T3SS2 secreted proteins, indicating that these genes are involved in qualitative and quantitative secretory regulation of Vibrio parahaemolyticus T3SS2.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (46 results)

All 2013 2012 2011

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (30 results) (of which Invited: 1 results)

  • [Journal Article] Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae2013

    • Author(s)
      Morita M, Yamamoto S, Hiyoshi H, Kodama T,Okura M, Arakawa E, Alam M, Ohnish M,Izumiya H, Watanabe H
    • Journal Title

      Microbiol Immunol

      Volume: 57(5) Issue: 5 Pages: 334-9

    • DOI

      10.1111/1348-0421.12039

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vibrio parahaemolyticus effector proteins suppress inflammasome activation by interfering with host autophagy signaling2013

    • Author(s)
      Naomi Higa, Claudia Toma, Yukiko Koizumi, Noboru Nakasone, Toshitsugu Nohara and Toshihiko Suzuki
    • Journal Title

      PloS Pathogen

      Volume: 9(1) Issue: 1 Pages: e1003142-e1003142

    • DOI

      10.1371/journal.ppat.1003142

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Horizontal gene transfer of a genetic island encoding a type III secretion system distributed in Vibrio cholerae.2013

    • Author(s)
      Morita M, Yamamoto S, Hiyoshi H, Kodama T, Okura M, Arakawa E, Alam M, Ohnishi M, Izumiya H, Watanabe H.
    • Journal Title

      Microbiol Immunol.

      Volume: Epub ahead of print Pages: n/a-n/a

    • DOI

      10.1111/j.1348-0421.12039

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in Escherichia coli K-12 strain as a molecular syringe.2012

    • Author(s)
      Akeda Y, Kimura T, Yamasaki A, Kodama T, Iida T, Honda T, Oishi K.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 427(2) Issue: 2 Pages: 242-7

    • DOI

      10.1016/j.bbrc.2012.09.018

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes.2012

    • Author(s)
      Matsuda S, Okada N, Kodama T, Honda T, Iida T.
    • Journal Title

      PLoS pathogens.

      Volume: 8(7) Issue: 7 Pages: e1002803-e1002803

    • DOI

      10.1371/journal.ppat.1002803

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hydrophilic translocator for Vibrio parahaemolyticus T3SS2 is also translocated.2012

    • Author(s)
      Zhou X, Ritchie MJ, Hiyoshi H, Iida T, Davis NB, Waldor KM, Kodama T.
    • Journal Title

      Infect Immun.

      Volume: 80(8) Issue: 8 Pages: 2940-7

    • DOI

      10.1128/iai.00402-12

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vibrio parahaemolyticus infection2012

    • Author(s)
      Kodama T
    • Journal Title

      Antibiotics and Chemotherapy

      Volume: 28(6) Pages: 63-70

    • URL

      https://www.iyaku-j.com/iyakuj/system/M2-1/summary_viewer.php?trgid=24993

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Hemolysin E- and enterohemolysin-derived hemolytic activity of O55/O157 strains and other Escherichia coli lineages2012

    • Author(s)
      K.Murase, et al
    • Journal Title

      Microbiology

      Volume: 158 Issue: 3 Pages: 746-758

    • DOI

      10.1099/mic.0.054775-0

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Inflammation and Disintegration of Intestinal Villi in an Experimental Model for Vibrio parahaemolyticus-Induced Diarrhea2011

    • Author(s)
      Ritchie JM, Rui H, Zhou X, Iida T, Kodoma T, et al
    • Journal Title

      PLoS Patnogens

      Volume: 8(3) Issue: 3 Pages: e1002593-e1002593

    • DOI

      10.1371/journal.ppat.1002593

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] an F-Actin-Binding Type III secretion effector, is Responsible for Vibrio parahaemolyticus-Induced enterotoxicity2011

    • Author(s)
      Hiyoshi H, Kodama T*, Saito K, Gotoh K, Matsuda S, Akeda Y, Honda T, and Iida T. VopV
    • Journal Title

      Cell Host Microbe

      Volume: 4 Pages: 401-19

    • URL

      http://dx.doi.org/10.1016/j.chom.2011.08.014

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the Vibrio parahaemolyticus Type III Secretion System 2-Associated Chaperone VocC for the T3SS2-Specific Effector VopC2011

    • Author(s)
      Yukihiro Akeda, Toshio Kodama, Kazunobu Saito, Tetsuya Iida, Kazunori Oishi and Takeshi Honda
    • Journal Title

      FEMS Microbiology Letters

      Volume: 324 Issue: 2 Pages: 156-164

    • DOI

      10.1111/j.1574-6968.2011.02399.x

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to β4-integrin expressing cell lines2011

    • Author(s)
      Abolghait SK, Iida T, Kodama T, Cantar elli VV, Akeda Y, Honda T
    • Journal Title

      Microb Pathog

      Volume: 51 Pages: 454-465

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between Localization on Cellular Membranes and Cytotoxicity of Vibrio vulnificus Hemolysin2011

    • Author(s)
      Sugiyama H, Kashimoto T, Ueno S, Ehara H, Kodama T, Iida T, Susa N
    • Journal Title

      PLoS One

      Volume: 6(10) Issue: 10 Pages: e26018-e26018

    • DOI

      10.1371/journal.pone.0026018

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Haemolysin E- and enterohaemolysin-derived haemolytic activity of O55/O157 strains and other Escherichia coli lineages2011

    • Author(s)
      Murase K, Ooka T, Iguchi A, Ogura Y, Nakayama K, Asadulghani M, Islam MR, Hiyoshi H, Kodama T, Beutin L, Hayashi T.
    • Journal Title

      Microbiology

      Volume: 158(Pt3) Pages: 746-758

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] VopV, an F-Actin-Binding Type III Secretion Effector, Is Required for Vibrio parahaemolyticus-Induced Enterotoxicity2011

    • Author(s)
      Hirotaka Hiyoshi, Toshio Kodama, Kazunobu Saito, Kazuyoshi Gotoh, Shigeaki Matsuda, Yukihiro Akeda, Takeshi Honda and Tetsuya Iida
    • Journal Title

      Cell Host & Microbe

      Volume: 10 Issue: 4 Pages: 401-409

    • DOI

      10.1016/j.chom.2011.08.014

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Recombinant AexU effector protein of Aeromonas veronii bv. sobria disrupts the actin cytoskeleton by downregulation of Rac1 and induces direct cytotoxicity to・4-integrin expressing cell lines2011

    • Author(s)
      Said Kamal Abolghait, Tetsuya Iida, Toshio Kodama, Vlademir V. Cantarelli, Yukihiro Akeda and Takeshi Honda
    • Journal Title

      Microbial Pathogenesis

      Volume: 51 Issue: 6 Pages: 454-465

    • DOI

      10.1016/j.micpath.2011.09.006

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] VepA, a cytotoxic type III effector of Vibrio parahaemolyticus, targets V-ATPase subunit c2013

    • Author(s)
      Shigeaki Matsuda
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] VopO, a T3SS2 effector of Vibrio, reorganizes actin cytoskeleton via activation of RhoA-ROCK pathway2013

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] VopC, a Vibrio parahaemolyticus T3SS2 effector, mediates invasion and actin stress fiber formation2013

    • Author(s)
      Ryu Okada
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Mechanism of Vibrio parahaemolyticus-induced enterotoxicity2013

    • Author(s)
      Toshio Kodama
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in E. coli as a tool2013

    • Author(s)
      Yukihiro Akeda
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] VepA, a cytotoxic type III effector of Vibrio parahaemolyticus, targets V-ATPase subunit c2013

    • Author(s)
      Shigeaki Matsuda
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] VopO, a T3SS2 effector of Vibrio, reorganizes actin cytoskeleton via activation of RhoA-ROCK pathway2013

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] VopC, a Vibrio parahaemolyticus T3SS2 effector, mediates invasion and actin stress fiber formation2013

    • Author(s)
      Ryu Okada
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mechanism of Vibrio parahaemolyticus-induced enterotoxicity2013

    • Author(s)
      Toshio Kodama
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Functional cloning of Vibrio parahaemolyticus type III secretion system 1 in E. coli as a tool2013

    • Author(s)
      Yukihiro Akeda
    • Organizer
      日本細菌学会 総会
    • Place of Presentation
      幕張メッセ 国際会議場(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Relationship between localization and cytotoxicity of Vibrio vulnificus hemolysin2012

    • Author(s)
      柏本孝茂
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Year and Date
      2012-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規F-アクチン結合T3SSエフェクターVopVは腸炎ビブリオの下痢原性に寄与する2012

    • Author(s)
      日吉大貴
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Year and Date
      2012-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H-ATPase subunit c and ruptures host cell lysosomes2012

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      US-Japan cooperative medical science program
    • Place of Presentation
      千葉大学(千葉県)
    • Related Report
      2012 Final Research Report
  • [Presentation] 腸炎ビブリオ3型分泌装置1エフェクターVepAによる細胞毒性の解析2012

    • Author(s)
      松田重輝
    • Organizer
      腸炎ビブリオシンポジウム
    • Place of Presentation
      日本文理大学 湯布院研修所(大分県)
    • Related Report
      2012 Final Research Report
  • [Presentation] VopV, an F-actin-binding type III secretion effector, is required for Vibrio parahaemolyticus-induced enterotoxicity2012

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      Awaji international forum on infection and immunity
    • Place of Presentation
      Awaji Yumebutai international conference center (Hyogo)
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターVopVの解析2012

    • Author(s)
      日吉大貴
    • Organizer
      毒素シンポジウム
    • Place of Presentation
      とかちプラザ(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Vibrio vulnificus hemolysin associates with detergent-resistant membranes, but this localization is not essential for its cytotoxicity2012

    • Author(s)
      Takashige Kashimoto
    • Organizer
      ASM general meeting
    • Place of Presentation
      San Francisco (USA)
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] An novel F-actin-binding type III secretion effector is required for Vibrio parahaemolyticus-induced enterotoxicity2012

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      ASM general meeting
    • Place of Presentation
      San Francisco (USA)
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 腸炎ビブリオ3型分泌装置1エフェクターVepAによる細胞毒性の解析2012

    • Author(s)
      松田重輝
    • Organizer
      腸炎ビブリオシンポジウム
    • Place of Presentation
      日本文理大学 湯布院研修所(大分県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H-ATPase subunit c and ruptures host cell lysosomes2012

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      US-Japan cooperative medical science program
    • Place of Presentation
      千葉大学(千葉県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターVopVの解析2012

    • Author(s)
      日吉大貴
    • Organizer
      毒素シンポジウム
    • Place of Presentation
      とかちプラザ(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Relationship between localization and cytotoxicity of Vibrio vulnificus hemolysin2012

    • Author(s)
      柏本孝茂、杉山寛幸、上野俊治、江原勇登、児玉年央、飯田哲也、諏佐信行
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 新規F-アクチン結合T3SSエフェクターVopVは腸炎ビブリオの下痢原性に寄与する2012

    • Author(s)
      日吉大貴、児玉年央、斉藤和伸、後藤和義、松田重輝、明田幸宏、本田武司、飯田哲也
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Related Report
      2011 Research-status Report
  • [Presentation] A novel F-actin-binding T3SS effector is responsible for Vibrio parahaemolyticus- induced diarrhea2011

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      US-Japan cooperative medical science program
    • Place of Presentation
      National Institute of Cholera and Enteric Diseases (India)
    • Year and Date
      2011-12-15
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターの解析2011

    • Author(s)
      日吉大貴
    • Organizer
      日本細菌学会関西支部総会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府)
    • Year and Date
      2011-11-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Hemolysin E- and Enterohemolysin-derived hemolytic activities of the O55/0157 and other Escherichia coli lineages2011

    • Author(s)
      Kazunori Murase
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel F-actin binding T3SS effector is responsible for Vibrio parahaemolyticus-induced diarrhea2011

    • Author(s)
      Hirotaka Hiyoshi
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 腸炎ビブリオの下痢原性に寄与する新規F-アクチン結合T3SSエフェクターの解析2011

    • Author(s)
      日吉大貴、児玉年央、斉藤和伸、後藤和義、松田重輝、明田幸宏、本田武司、飯田哲也
    • Organizer
      日本細菌学会関西支部総会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hemolysin E- and Enterohemolysin-derived hemolytic activities of the O55/0157 and other Escherichia coli lineages2011

    • Author(s)
      Kazunori Murase, Tadasuke Ooka, Yoshitoshi Ogura, Atsushi Iguchi, Keisuke Nakayama, Md Asadulghani, Md Rakibul Islam, Hirotaka Hiyoshi, Toshio Kodama, Lothar Beutin, Tetsuya Hayashi
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] A novel F-actin binding T3SS effector is responsible for Vibrio parahaemolyticus-induced diarrhea2011

    • Author(s)
      Hirotaka Hiyoshi, Toshio Kodama, Takeshi Honda, Tetsuya Iida
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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