Genome analysis of bacteriophage toward botulinum neurotoxin-transfer mechanism

• Project/Area Number: 23790479
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: SAKAGUCHI Yoshihiko 宮崎大学, IR 推進機構, 助教 (70403491)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ボツリヌス菌 / ボツリヌス毒素遺伝子 / バクテリオファージ / プラスミド / ファージ変換 / 接合伝達 / チューブリン / 分配 / 人獣共通感染症 / 伝達 / ゲノム解析 / 構造タンパク質

Research Abstract

Phage c-st is typical Clostridium botulinumtype C neurotoxin-converting phage. Although the phage c-st genome has been sequenced, no much information is available in its biology and genome. To extend our understanding of horizontal gene transfer by this phage, we investigated in the core genome of phage c-st. First, analyzing the phage structural proteins, the genome module for the structural proteins was 98.5-157.5 kbp of phage c-st genome. Second, comparing the genome of phage c-st with that of a related phage c-468, the 43-57 kbp and 116.5-123 kbp of phage c-st genome were very similar to phage 468 genome. Third, the essential genes for phage genome partitioning were revealed to be located 175.5-178.5 kbp of phage c-st genome. According to these results, I speculated that the core genome was the 1-43 kbp, 57-116.5 kbp, and 123-186 kbp regions of phage c-st genome.

Research Products

• [Journal Article] Phospholipase C produced by Clostridium botulinumtypes C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin (2013)
  • Author(s): 2Fatmawati, N. N., Sakaguchi, Y., Suzuki, T., Oda, M., Shimizu, K., Yamamoto, Y., Sakurai, J., Matsushita, O., Oguma, K.
  • Journal Title: Acta Med. Okayama Volume: 67(1) Pages: 9-18
  • URL: http://www.lib.okayama-u.ac.jp/www/acta/pdf/67_1_9.pdf
  • Peer Reviewed
• [Journal Article] Phospholipase C produced by Clostridium botulinum types C and D: comparison of gene, enzymatic, and biological activities with those of Clostridium perfringens alpha-toxin (2013)
  • Author(s): Fatmawati, N.N.D
  • Journal Title: Acta Med. Okayama Volume: 67(1) Pages: 9-18
  • Peer Reviewed
• [Journal Article] 1Oliva, M. A., Martin-Galiano, A. J., Sakaguchi, Y., Andreu, J. M (2012)
  • Author(s): 1Oliva, M. A., Martin-Galiano, A. J., Sakaguchi, Y., Andreu, J. M
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 109(20) Pages: 7711-7716
  • Peer Reviewed
• [Journal Article] Tubulin homolog TubZ in a phage-encoded partition system (2012)
  • Author(s): Oliva, M. A.
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A. Volume: 109(20) Issue: 20 Pages: 7711-7716
  • DOI: 10.1073/pnas.1121546109
  • Peer Reviewed
• [Journal Article] Tubulin homolog TubZ in a phage-encoded partition system (2012)
  • Author(s): Oliva, MA., Martin-Galiano, AJ., Sakaguchi, Y., Andreu, JM.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: In Press
  • Peer Reviewed
• [Journal Article] TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding (2011)
  • Author(s): Sakaguchi M
  • Journal Title: PLoS One Volume: 6 Issue: 8 Pages: e23132-e23132
  • DOI: 10.1371/journal.pone.0023132
  • Peer Reviewed
• [Journal Article] TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding (2011)
  • Author(s): Sakaguchi, M., Murata, H., Yamamoto, K., Ono, T., Sakaguchi, Y., Motoyama, A., Hibino, T., Kataoka, K., Huh, NH.
  • Journal Title: PLoS One Volume: 6(8)
  • Peer Reviewed
• [Presentation] The search for core-genome of Clostridium botulinumbacteriophage converting type C neurotoxin (2013)
  • Author(s): Sakaguchi, Y., Uchiyama, J., Ogura, Y., Yamamoto, Y., Suzuki, T., Orita, K., Tsuda, C., Matsuzaki, S., Hayashi, T., Oguma, K
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] The complete sequence of plasmid encoding neurotoxin gene in Clostridium botulinumtype B strain (2013)
  • Author(s): Hosomi, K., Sakaguchi, Y., Goto, K., Nakamura, K., Kohda, T., Mukamoto, M., Iida, T., Ogura, Y., Hayashi, T., Kozaki, S.
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] Morphological and genomic analysis of Helicobacter pyloribacteriophage KHP30 (2013)
  • Author(s): Uchiyama, J., Takeuchi, H., Sakaguchi, Y., Ujihara, T., Uchiyama, I., Daibata, M., Matsuzaki, S
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] The search for core-genome of Clostridium botulinum bacteriophage converting type C neurotoxin (2013)
  • Author(s): Yoshihiko Sakaguchi
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] The complete sequence of plasmid encoding neurotoxin gene in Clostridium botulinum type B strain (2013)
  • Author(s): Koji Hosomi
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] Morphological and genomic analysis of Helicobacter pylori bacteriophage KHP30 (2013)
  • Author(s): Jumpei Uchiyama
  • Organizer: The 86th Annual Meeting of Japanese Society for Bacteriology
  • Place of Presentation: Chiba
• [Presentation] C型ボツリヌス毒素変換ファージの構造タンパク質の解析 (2012)
  • Author(s): 阪口義彦、内山淳平、小椋義俊、山本由弥子、鈴木智典、阪口政清、織田華絵、津田千秋、松崎茂展、林 哲也、小熊惠二
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
• [Presentation] Improved adsorption of Enterococcus faecalisbacteriophage ΦEF24C caused by a point mutation in a tail fiber gene (2012)
  • Author(s): Uchiyama, J., Sakaguchi, Y., Takemura-Uchiyama, I., Kato, S., Satoh, M., Ujihara, T., Daibata, M., Matsuzaki, S
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
• [Presentation] ピロリ菌バクテリオファージの分離・解析 (2012)
  • Author(s): 内山淳平、竹内 啓晃、加藤伸一郎、阪口義彦、蒲生啓司、氏原隆子、内山伊代、大畑雅典、松崎茂展
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
• [Presentation] Analysis of pseudolysogenic lifecycle of Clostridium botulinumphage c-st, based on comparative genomics of wild-type and long-term-incubated mutants phages (2012)
  • Author(s): 4Sakaguchi, Y., Oliva, M. A., Martin-Galiano, A. J., Andreu, J. M., Sakaguchi, M., Uchiyama, J., Ogura, Y., Yamamoto, Y., Suzuki, T., Orita, K., Tsuda, C., Matsuzaki, S., Hayashi, T., Oguma, K.
  • Organizer: The 35th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
• [Presentation] Improved adsorption of Enterococcus faecalis bacteriophage ΦEF24C caused by a point mutation in a tail fiber gene (2012)
  • Author(s): Jumpei Uchiyama
  • Organizer: 第4回ファージ研究会
  • Place of Presentation: 前橋市
• [Presentation] Analysis of pseudolysogenic lifecycle of Clostridium botulinum phage c-st, based on comparative genomics of wild-type and long-term-incubated mutants phages (2012)
  • Author(s): Yoshihiko Sakaguchi
  • Organizer: The 35th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Fukuoka
• [Book] 医薬ジャーナル社「病原菌の今日的意味 改訂 4 版 (2011)
  • Author(s): 小熊惠二、阪口義彦、西河 淳
  • Publisher: ボツリヌス菌 (Clostridium botulinum)
• [Book] 医薬ジャーナル社「病原菌の今日的意味 改訂4版」 (2011)
  • Author(s): 小熊惠二, 阪口義彦, 西河 淳
  • Publisher: ボツリヌス菌 (Clostridium botulinum)
• [Remarks] 宮崎大学型若手研究リーダー育成モデル
  • URL: http://www.miyazaki-u.ac.jp/ir/?p=92
• [Remarks] 宮崎大学型若手研究リーダー育成モデル＿IRO特任助教の紹介
  • URL: http://www.miyazaki-u.ac.jp/ir/?p=92
• [Remarks]
  • URL: http://www.miyazaki-u.ac.jp/ir/