Genome and virulence mechanism of an emerging intestinal pathogen, Escherichia albertii.

• Project/Area Number: 23790480
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: University of Miyazaki
• Principal Investigator: OOKA Tadasuke 宮崎大学, 医学部, 助教 (50363594)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 遺伝 / ゲノム / Escherichia albertii / ゲノム解析 / 病原性 / 疫学ツール / 細菌 / 病原機構 / III型分泌システム

Research Abstract

Escherichia albertiiis an emerging intestinal pathogen. We determined the complete genome sequences of 4 E. albertiistrains and identified a variety of virulence factors such as LEE-encoded type III secretion system (T3SS), T3SS-secreted effectors, cytolethal distending toxins, and adhesion factors. In addition, the genomic comparison among E. albertiistrains or between E. albertiiand E. colirevealed the genome features of E. albertiispecies.

Research Products

• [Journal Article] A human outbreak case associated with Escherichia albertii (2013)
  • Author(s): T. Ooka, E. Tokuoka, M. Fukuhara, T. Nagamura, Y. Ogura, K. Aisawa, S. Harada, T. Hayashi
  • Journal Title: Emerg. Infect. Dis. Volume: 19 Issue: 1 Pages: 144-146
  • DOI: 10.3201/eid1901.120646
  • URL: http://dx.doi.org/
  • Peer Reviewed
• [Journal Article] Clinical significance of Escherichia albertii.Emerg (2012)
  • Author(s): T.Ooka, et al
  • Journal Title: Emerg.Infect.Dis. Volume: (in press) Issue: 3 Pages: 488-492
  • DOI: 10.3201/eid1803.111401
  • URL: http://dx.doi.org/
  • Peer Reviewed
• [Journal Article] Is bile acid a determinant of the gut microbiota on a high-fat diet? (2012)
  • Author(s): A. Yokota, S. Fukiya, T. Ooka, Y. Ogura, T. Hayashi, S. Ishizuka
  • Journal Title: Gut Microbes Volume: 3(5) Issue: 5 Pages: 455-459
  • DOI: 10.4161/gmic.21216
  • URL: http://dx.doi.org/
  • Peer Reviewed
• [Journal Article] 腸管出血性大腸菌O111の進化と病原性 (2012)
  • Author(s): 小椋義俊, 大岡唯祐, 林哲也
  • Journal Title: 神経内科 Volume: 76(2) Pages: 155-163
• [Presentation] 新規下痢原性病原菌Escherichia albertiiのゲノム解析 (2013)
  • Author(s): 大岡唯祐, 後藤恭宏, 小椋義俊, 林哲也
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] Escherichia属の新菌種E. albertiiのゲノム解析 (2013)
  • Author(s): 大岡唯祐, 桂啓介, 後藤恭宏, 小椋義俊, 林哲也
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 滋賀県長浜市
• [Presentation] 新興病原体Escherichia albertiiのゲノム解析 (2012)
  • Author(s): 大岡唯祐, 勢戸和子, 河野喜美子, 小林秀樹, 江藤良樹, 市原祥子, 瀬戸順次, 磯部順子, 山口敬治, 堀川和美, 小椋義俊, 林哲也
  • Organizer: 第16回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 秋田市
• [Presentation] Significant prevalence of Escherichia albertiiamong the strains identified as enteropathogenic and enterohemorrhagic E. coliand the presence of Shiga toxin-producing E. albertii strains. (2012)
  • Author(s): T. Ooka, K. Seto, K. Kawano, H. Kobayashi, Y. Etoh, S. Ichihara, N. Kaneko, J. Isobe, K. Yamaguchi, K. Horikawa, T.A. Gomes, A. Linden, M. Bardiau, J.G. Mainil, L. Beutin, Y. Ogura, T. Hayashi
  • Organizer: VTEC 2012: 8thInternational Symposium on Shiga Toxin (Verocytotoxin) producing Escherichia coli infections
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] 志賀毒素バリアントStx2fの伝播様式の解析 (2012)
  • Author(s): 大岡唯祐,勢戸和子,磯部順子,山口敬治, 瀬戸順次, 小椋義俊, 林哲也
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎市
• [Presentation] Significant prevalence of Escherichia albertii among the strains identified as enteropathogenic and enterohemorrhagic E. coli and the presence of Shiga toxin-producing E. albertii strains. (2012)
  • Author(s): T. Ooka, K. Seto, K. Kawano, H. Kobayashi, Y. Etoh, S. Ichihara, N. Kaneko, J. Isobe, K. Yamaguchi, K. Horikawa, T.A. Gomes, A. Linden, et al.
  • Organizer: VTEC 2012: 8th International Symposium on Shiga Toxin (Verocytotoxin) producing Escherichia coli infections
  • Place of Presentation: Amsterdam, The Netherlands.
• [Presentation] 志賀毒素バリアントStx2fの伝播様式の解析 (2012)
  • Author(s): 大岡唯祐、勢戸和子、磯部順子、山口敬治、瀬戸順次、小椋義俊、林哲也
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール（長崎）
• [Presentation] Phylogenetic and intimin-subtyping analysis of enteropathogenic and enterohemorrhagic Escherichia colistrains (2011)
  • Author(s): T. Ooka, K. Seto, K. Kawano, H. Kobayashi, Y. Etoh, S. Ichihara, M. Bardiau, N. Kaneko, J. Isobe, K. Yamaguchi, K. Horikawa, Y. Ogura, K. Nakayama, J. Mainil, T. Hayashi
  • Organizer: IUMS 2011(第84回日本細菌学会総会)
  • Place of Presentation: Sapporo.
• [Presentation] 種々のeae遺伝子保有株におけるEscherichia albertiiの分布とその性状解析 (2011)
  • Author(s): 大岡唯祐,勢戸和子,河野喜美子,小林秀樹,江藤良樹,市原祥子,金子紀子,磯部順子,山口敬治,堀川和美,小椋義俊,林哲也
  • Organizer: 第64回日本細菌学会九州支部総会
  • Place of Presentation: 北九州市
• [Presentation] eae遺伝子保有株におけるEscherichia albertiiの分布及びその性状と ゲノム特性 (2011)
  • Author(s): 大岡唯祐、勢戸和子、河野喜美子、小林秀樹、江藤良樹、市原祥子、金子紀子、磯部順子、山口敬治、堀川和美、小椋義俊、林哲也
  • Organizer: 第15回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 北浜フォーラム（大阪）
• [Presentation] Phylogenetic and intimin-subtyping analysis of eae-positive strains initially identified as enteropathogenic and enterohemorrhagic Escherichia coli. (2011)
  • Author(s): Ooka T, Seto K, Kawano K, Kobayashi H, Etoh Y, Ichihara S, Bardiau M, Kaneko A, Isobe J, Yamaguchi K, Horikawa K, Ogura Y, Nakayama K, Mainil J, Hayashi T
  • Organizer: XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: Sapporo Convention Center（札幌）