Characterization of bacterial collagen sensor and adhesin for screening of new matrix anchors

• Project/Area Number: 23790483
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Okayama University (2012-2013); Kitasato University (2011)
• Principal Investigator: MIMA Takehiko 岡山大学, 医歯(薬)学総合研究科, 助教 (80596437)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: コラゲナーゼ / ビブリオ / 発現調節 / 二成分制御系 / 遺伝子発現 / 毒素 / Two-component system / Vibrio / V. alginolyticus / 発現誘導 / コラーゲン / マトリックス・アンカー / alginolyticus

Research Abstract

Vibrio alginolyticus produces collagenase only when collagen is present. This shows that V. alginolyticus possesses the sensor for collagen. Isolating mutants that are deficient in collagenase production using newly invented method identified genes that regulate production of collagenase in V. alginolyticus. As the result, two-component regulatory system was identified to regulate the expression of collagenase in V. alginolyticus.

Research Products

• [Journal Article] Acceleration of periosteal bone formation by human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase. (2014)
  • Author(s): Uchida K, Matsushita O, Naruse K, Mima T, Nishi N, Hattori S, Ogura T, Inoue G, Tanaka K, Takaso M.
  • Journal Title: J Biomed Mater Res A. Volume: 102 Issue: 6 Pages: 1737-1743
  • DOI: 10.1002/jbm.a.34841
  • Peer Reviewed
• [Journal Article] Characterization of d-boroAla as a Novel Broad-Spectrum Antibacterial Agent Targeting d-Ala-d-Ala Ligase (2011)
  • Author(s): Putty S, Rai A, Jamindar D, Pagano P, Quinn CL, Mima T, Schweizer HP, Gutheil WG
  • Journal Title: Chem Biol Drug Des Volume: 78 Issue: 5 Pages: 757-763
  • DOI: 10.1111/j.1747-0285.2011.01210.x
  • Peer Reviewed
• [Journal Article] In vitro activity of cethromycin against Burkholderia pseudomallei and investigation of mechanism of resistance (2011)
  • Author(s): Mima T, Schweizer HP, Xu ZQ
  • Journal Title: J Antimicrob Chemother Volume: 66 Issue: 1 Pages: 73-78
  • DOI: 10.1093/jac/dkq391
  • Peer Reviewed
• [Journal Article] In vitro activity of BAL30072 against Burkholderia pseudomallei (2011)
  • Author(s): Mima T, Kvitko BH, Rholl DA, Page MG, Desarbre E, Schweizer HP
  • Journal Title: Int J Antimicrob Agents Volume: 38 Issue: 2 Pages: 157-159
  • DOI: 10.1016/j.ijantimicag.2011.03.019
  • Peer Reviewed
• [Journal Article] Characterization of dense artificial connective tissues generated in a newly designed bioreactor (2011)
  • Author(s): Iwashiro H, Hosoya S, Hirai K, Mima T, Ohashi S, Aihara T, Ito S, Ohara S, Adachi E
  • Journal Title: Connect Tissue Res Volume: 52 Pages: 340-352
  • Peer Reviewed
• [Journal Article] Characterization of dense artificial connective tissues generated in a newly designed bioreactor (2011)
  • Author(s): H. Iwashiro, S. Hosoya, K. Hirai, T. Mima, S. Ohashi, T. Aihara, S. Ito, S. Ohara, and E. Adachi
  • Journal Title: Connect Tissue Res Volume: 52 (4) Issue: 4 Pages: 340-352
  • DOI: 10.3109/03008207.2010.531801
  • Peer Reviewed
• [Presentation] Drug delivery based on clostridial collagenase showed therapeutic effects in orthopedic surgery (2014)
  • Author(s): 松下治、美間健彦、山本由弥子、鈴木智典、横田憲治
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-26
• [Presentation] ピロリ菌抗体陰性早期胃がん患者における抗体価の検討 (2014)
  • Author(s): 横田憲治、鈴木綾、美間健彦、鈴木智典、山本由弥子、松下治、小熊惠二、林俊治、平井義一
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-26
• [Presentation] 三叉神経におけるA型ボツリヌス神経毒素の取り込みと軸索輸送 (2014)
  • Author(s): 山本由弥子、鈴木智典、美間健彦、横田憲治、松下治、小熊惠二
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-03-26
• [Presentation] Vibrio alginolyticusのVarS/VarAに二成分制御系によるコラゲナーゼ発現調節機構の解析 (2014)
  • Author(s): 美間健彦、鈴木智典、山本由弥子、横田憲治、松下治
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Vibrio alginolyticusのVarS/VarA二成分制御系によるコラゲナーゼ発現調節機構の解析 (2014)
  • Author(s): 美間健彦、前田惠子、鈴木智典、山本由弥子、横田憲治、松下 治
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Vibrio alginolyticusのコラゲナーゼの発現誘導機構の解明 (2013)
  • Author(s): 美間健彦
  • Organizer: 第1回感染研-岡山大連携大学院シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2013-12-18
• [Presentation] Vibrio alginolyticusのコラゲナーゼの発現誘導機構の解明 (2013)
  • Author(s): 美間健彦、前田惠子、白川拓、鈴木智典、山本由弥子、横田憲治、徳光浩、松下治
  • Organizer: 第47回腸炎ビブリオシンポジウム
  • Place of Presentation: 広島
  • Year and Date: 2013-11-15
• [Presentation] Vibrio alginolyticusのコラゲナーゼの発現誘導機構の解明 (2013)
  • Author(s): 前田惠子、美間健彦、鈴木智典、山本由弥子、横田憲治、松下治
  • Organizer: 第66回日本細菌学会中国・四国支部総会
  • Place of Presentation: 広島
  • Year and Date: 2013-10-12
• [Presentation] Helicobacter pylori感染による胃がん検診(ABC検診)の改良 (2013)
  • Author(s): 横田憲治、鈴木綾、小熊惠二、前田惠子、美間健彦、鈴木智典、山本由弥子、松下治
  • Organizer: 第66回日本細菌学会中国・四国支部総会
  • Place of Presentation: 広島
  • Year and Date: 2013-10-12
• [Presentation] Differential targeting mechanism of prototypical collagen binding domains from clostridial collagenase (2013)
  • Author(s): Joshua Sakon、Keisuke Tanaka、Rohana Liyanage、Jackson O. Lay, Jr、Ryan Bauer、Takehiko Mima、Shunji Hattori、Osamu Matsushita
  • Organizer: GRC-collage
  • Place of Presentation: New Hampshire (USA)
• [Presentation] Identification of binding sequence on porcine type I collagen by collagen-binding domain from Clostridial collagenase ColG (2013)
  • Author(s): Keisuke Tanaka、Rohana Liyanage、Jackson O. Lay, Jr、Ryan Bauer、Takehiko Mima、Shunji Hattori、Osamu Matsushita and Joshua Sakon
  • Organizer: GRC-collage
  • Place of Presentation: New Hampshire (USA)
• [Presentation] Vibrio alginolyticusのcollagenase産生誘導機構の解明 (2013)
  • Author(s): 美間健彦、鈴木智典、山本由弥子、横田憲治、松下治
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Structural comparison of collagen-binding domain derived from Clostridium histolyticum collagenases (2013)
  • Author(s): 松下治、美間健彦、鈴木智典、山本由弥子、横田憲治
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] E型ボツリヌス毒素は、A型ボツリヌス毒素と異なるメカニズムによりCaco-2細胞へ結合する (2013)
  • Author(s): 山本由弥子、鈴木智典、美間健彦、藤田久美子、横田憲治、小熊惠二、松下治
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Helicobacter pylori日本人株抗原に対するIgG抗体価の検討 (2013)
  • Author(s): 横田憲治、松下治、山本由弥子、鈴木智典、美間健彦、小熊惠二、林俊治、平井義一
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 東京
• [Presentation] Differential targeting mechanism of prototypical collagen binding domains from clostridial collagenase (2013)
  • Author(s): Joshua Sakon, Keisuke Tanaka, Rohana Liyanage, Jackson O. Lay, Jr, Ryan Bauer, Takehiko Mima, Shunji Hattori, Osamu Matsushita
  • Organizer: Gordon Research Conferences
  • Place of Presentation: New London, NH, USA
• [Presentation] Identification of binding sequence on porcine type I collagen by collagen-binding domain from Clostridial collagenase ColG (2013)
  • Author(s): Keisuke Tanaka, Rohana Liyanage, Jackson O. Lay, Jr, Ryan Bauer, Takehiko Mima, Shunji Hattori, Osamu Matsushita, Joshua Sakon
  • Organizer: Gordon Research Conferences
  • Place of Presentation: New London, NH, USA
• [Presentation] Helicobacter pylori感染による胃がん検診（ABC検診）の改良 (2013)
  • Author(s): 横田憲治、鈴木 綾、小熊惠二、前田惠子、美間健彦、鈴木智典、山本由弥子、松下 治
  • Organizer: 第66回日本細菌学会中国・四国支部総会
  • Place of Presentation: 広島
• [Presentation] Vibrio alginolyticusのcollagenase産生誘導機構の解明 (2013)
  • Author(s): 美間健彦、鈴木智典、山本由弥子、横田健司、小出隆規、松下治
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Structural comparison of collagen-binding domains from Clostridium histolyticum collagenase (2013)
  • Author(s): 松下治、美間健彦、鈴木智典、山本由弥子、横田健司
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] E型ボツリヌス毒素は、A型ボツリヌス毒素と異なるメカニズムによりCaco-2細胞へ結合する (2013)
  • Author(s): 山本由弥子、鈴木智典、美間健彦、藤田久美子、横田健司、小熊惠二、松下治
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Helicobacter pylori日本人株抗原に対するIgG抗体価の検討 (2013)
  • Author(s): 横田健司、松下治、山本由弥子、鈴木智典、美間健彦、小熊惠二、林俊治、平井義一
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Vibrio alginolyticusのcollagenase産生誘導機構の解明 (2012)
  • Author(s): 美間健彦、鈴木智典、山本由弥子、横田憲治、小出隆規、松下治
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-10-21
• [Presentation] ガス壊疽菌群コラゲナーゼの基質認識機構の解明と臨床応用 (2012)
  • Author(s): 松下治、美間健彦、鈴木智典、山本由弥子、横田憲治、小出隆規、安達栄治郎、玉井栄治、宮田茂、南純三郎、岡部昭延
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-10-21
• [Presentation] Bacterial collagen-binding domain–Molecular basics and clinical applications- (2012)
  • Author(s): 松下治、美間健彦、安達栄治郎、小出隆規
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] Bacterial collagen-binding domains -Molecular basics and clinical applications- (2012)
  • Author(s): 松下治、美間健彦、安達栄治郎、小出隆規
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎県長崎市
• [Presentation] Medical Application of Clostridial Toxins; 2)Collagenase (2011)
  • Author(s): Osamu Matsushita、Takehiko Mima、Eijiro Adachi、Joshua Sakon、Nozomu Nishi、Takenori Miyashita、Nozomu Mori、Joe Inoue、Masakazu Hattori、Minekatsu Akimoto、Akira Takeda
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 北海道
  • Year and Date: 2011-09-09
• [Presentation] Medical Application of Clostridial Toxins; 2)Collagenases (2011)
  • Author(s): Osamu Matsushita、Takehiko Mima、Eijiro Adachi、Joshua Sakon、Nozomu Nishi、Takenori Miyashita、Nozomu Mori、Joe Inoue、Masakazu Hattori、Minekatsu Akimoto、Akira Takeda
  • Organizer: 第84回日本細菌学会総会
  • Place of Presentation: 北海道札幌市
• [Presentation] Vibrio alginolyticusのcollagenase産生誘導機構の解明
  • Author(s): 美間健彦、鈴木智典、山本由弥子、横田健司、小出隆規、松下治
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島
• [Presentation] ガス壊疽菌群コラゲナーゼの基質認識機構の解明と臨床応用
  • Author(s): 松下治、美間健彦、鈴木智典、山本由弥子、横田健司、小出隆規、安達栄治郎、玉井英治、宮田茂、南純三郎、岡部昭延
  • Organizer: 第65回日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島
• [Remarks] 岡山大学大学院医歯薬学総合研究科病原細菌学分野ホームページ
  • URL: http://www.okayama-u.ac.jp/user/saikin/Bacteriology/Welcome.html
• [Remarks] 岡山大学大学院医歯薬学総合研究科病原細菌学分野ホームページ
  • URL: http://www.okayama-u.ac.jp/user/saikin/