Transposon mutagenesis of probiotic Lactobacillus casei identifies asnH, an asparagine synthetase gene that contributes to activation of host innate immunity

Research Project

Project/Area Number	23790485
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Bacteriology (including Mycology)
Research Institution	Kitasato University
Principal Investigator	ITO Masahiro 北里大学, 薬学部, 助教 (90596727)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	プロバイオティクス / 自然免疫 / 菌体表層構造 / 自然免疫賦活化
Research Abstract	We demonstrated that AsnH exhibits asparagine synthetase activity when the lysozyme-treated cell wall extract of L. casei was added as substrate. L. caseicarrying mutated asnH lacked its characteristic thick and rigid cell wall structure, indicating that AsnH of L. casei is involved in cell wall biosynthesis. These results indicate that asnH is required for bacterial cell wall biosynthesis and is involved in the probiotic activity of L. casei ATCC 27139.

Report

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