The Role of PdpC in determination of the virulence of Francisella tularensis
| Project/Area Number |
23790489
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
UDA AKIHIKO 国立感染症研究所, 獣医科学部, 主任研究官 (80392322)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Osamu 国立感染症研究所, 獣医科学部, 主任研究官 (20260276)
TANABAYASHI Kiyoshi 国立感染症研究所, 獣医科学部, 室長 (50197505)
SUGIURA Naoko 岐阜大連携大学院
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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|---|
| Keywords | Francisella / Pathogen / 人獣共通感染症 / 感染症 / 病原因子 / 病原性 / 野兎病 / 野兎病菌 / 人畜共通感染症 |
|---|
| Research Abstract |
Role of pathogenicity determinant protein C (PdpC) in determining the virulence of the Francisella tularensis was examined using the pdpC knockout and the complemented strains. These data showed that PdpC was an essential element determining F. tularensis pathogenicity. Especially, three C-terminal lysine residues at amino acid positions 1303, 1309 and 1324 of PdpC molecule were indispensable for the pathogenicity. The lysates of macrophages inoculated with F. tularensis were immunoprecipitated with anti-PdpC antibody to determine the interactive molecules with Franicsella PdpC. The data indicated that Francisella PdpC molecule would bind to nucleic acid. In addition, it was suggested that Francisella PdpC molecule in the macrophages inoculated with virulent Francisella tularensis strain could suppress the gene expression to eliminate the intracellular bacteria by microarray analysis.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Vector-Borne and Zoonotic Diseases2014
Author(s)
Sharma Neekun, Hotta Akitoyo, Yamamoto Yoshie, Uda Akihiko, Fujita Osamu, Mizoguchi Toshio, Shindo Junji, Park Chun-Ho, Kudo Noboru, Hatai Hitoshi, Oyamada Toshifumi, Yamada Akio, Morikawa Shigeru, and Tanabayashi Kiyoshi
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Journal Title
Vector-Borne and Zoonotic Diseases
Volume: 14(4)
Issue: 4
Pages: 234-239
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of the Source of <i>Francisella tularensis</i> Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis2013
Author(s)
Osamu Fujita, Akitoyo Hotta, Akihiko Uda, Yoshie Yamamoto, Hiromi Fujita, Fumiaki Shinya, Shigeyuki Asano, Shigeru Morikawa, Kiyoshi Tanabayashi, Akio Yamada
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Journal Title
Japanese Journal of Infectious Diseases
Volume: 66
Issue: 6
Pages: 543-545
DOI
NAID
ISSN
1344-6304, 1884-2836
Related Report
Peer Reviewed
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[Presentation] 抗野兎病菌抗体検出用競合ELISAの開発および野生動物血清調査への応用2013
Author(s)
Neekun Sharma,堀田明豊,棚林清,山本美江,藤田修,宇田晶彦,溝口俊夫,進藤順治,朴天鎬,畑井仁,工藤上, ,山田敏文,山田章雄,森川茂
Organizer
第156回日本獣医学会学術集会
Place of Presentation
岐阜
Related Report
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[Presentation] Development of competitive ELISA for serosurvey of tularemia among various animal species.2011
Author(s)
Sharma N, Hotta A, Tanabayashi K, Yamamoto Y, Fujita O, Uda A, Mizoguchi T, Shindo J, Park C H, Kudo N, Hatai H, Oyamada T, Yamada A
Organizer
5th Asian Workshop on Zoo and Wildlife Medicine/ Conservation in Nepal 2011
Place of Presentation
ネパール
Related Report
