The Role of PdpC in determination of the virulence of Francisella tularensis

• Project/Area Number: 23790489
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: UDA AKIHIKO 国立感染症研究所, 獣医科学部, 主任研究官 (80392322)
• Co-Investigator(Renkei-kenkyūsha): FUJITA Osamu 国立感染症研究所, 獣医科学部, 主任研究官 (20260276) ; TANABAYASHI Kiyoshi 国立感染症研究所, 獣医科学部, 室長 (50197505) ; SUGIURA Naoko 岐阜大連携大学院
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: Francisella / Pathogen / 人獣共通感染症 / 感染症 / 病原因子 / 病原性 / 野兎病 / 野兎病菌 / 人畜共通感染症

Research Abstract

Role of pathogenicity determinant protein C (PdpC) in determining the virulence of the Francisella tularensis was examined using the pdpC knockout and the complemented strains. These data showed that PdpC was an essential element determining F. tularensis pathogenicity. Especially, three C-terminal lysine residues at amino acid positions 1303, 1309 and 1324 of PdpC molecule were indispensable for the pathogenicity. The lysates of macrophages inoculated with F. tularensis were immunoprecipitated with anti-PdpC antibody to determine the interactive molecules with Franicsella PdpC. The data indicated that Francisella PdpC molecule would bind to nucleic acid. In addition, it was suggested that Francisella PdpC molecule in the macrophages inoculated with virulent Francisella tularensis strain could suppress the gene expression to eliminate the intracellular bacteria by microarray analysis.

Research Products

• [Journal Article] Role of Pathogenicity Determinant Protein C (PdpC) in Determining the Virulence of the Francisella tularensis Subspecies tularensis SCHU (2014)
  • Author(s): Uda A, Sekizuka T, Tanabayashi K, Fujita O, Kuroda M, Hotta A, Sugiura N, Sharma N, Morikawa S, Yamada A
  • Journal Title: PLoS ONE Volume: 9
  • DOI: 10.1371/journal.pone.0089075
  • Peer Reviewed
• [Journal Article] Serosurveillance for Francisella tularensis among wild animals in Japan using a newly developed competitive enzyme-linked immunosorbent assay (2014)
  • Author(s): Sharma Neekun, Hotta Akitoyo, Yamamoto Yoshie, Uda Akihiko, Fujita Osamu, Mizoguchi Toshio, Shindo Junji, Park Chun-Ho, Kudo Noboru, Hatai Hitoshi, Oyamada Toshifumi, Yamada Akio, Morikawa Shigeru, and Tanabayashi Kiyoshi
  • Journal Title: Vector Borne Zoonotic Dis Volume: 14(4) Pages: 234-239
  • DOI: 10.1089/vbz.2013.1349
  • Peer Reviewed
• [Journal Article] In Vitro Antibiotic Susceptibility of <i>Francisella tularensis</i> Isolates from Japan (2013)
  • Author(s): Akitoyo Hotta, Osamu Fujita, Akihiko Uda, Neekun Sharma , Kiyoshi Tanabayashi, Yoshie Yamamoto, Akio Yamada, Shigeru Morikawa
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 66(6) Issue: 6 Pages: 534-536
  • DOI: 10.7883/yoken.66.534
  • NAID: 130003390336
  • Peer Reviewed
• [Journal Article] Detection of Francisella tularensis-Specific Antibodies in Patients with Tularemia by a Novel Competitive Enzyme-Linked Immunosorbent Assay (2013)
  • Author(s): Sharma N, Hotta A, Yamamoto Y, Fujita O, Uda A, Morikawa S, Yamada A, Tanabayashi K.
  • Journal Title: Clin Vaccine Immunol Volume: 20 Pages: 9-16
  • DOI: 10.1128/cvi.00516-12
  • Peer Reviewed
• [Journal Article] Identification of the Source of <i>Francisella tularensis</i> Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis (2013)
  • Author(s): Osamu Fujita, Akitoyo Hotta, Akihiko Uda, Yoshie Yamamoto, Hiromi Fujita, Fumiaki Shinya, Shigeyuki Asano, Shigeru Morikawa, Kiyoshi Tanabayashi, Akio Yamada
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 66(6) Issue: 6 Pages: 543-545
  • DOI: 10.7883/yoken.66.543
  • NAID: 130003390339
  • Peer Reviewed
• [Journal Article] Seroprevalence of tularemia in wild bears and hares in Japan. (2012)
  • Author(s): Hotta A, Tanabayashi K, Yamamoto Y, Fujita O, Uda A, Mizoguchi T, et. Al
  • Journal Title: Zoonoses Public Health. Volume: 59 Pages: 89-95
  • DOI: 10.1111/j.1863-2378.2011.01422.x
  • Peer Reviewed
• [Journal Article] Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in mice (2011)
  • Author(s): Sugiura N, Uda A, Inoue S, Kojima D, Hamamoto N, Kaku Y, et. Al
  • Journal Title: Jpn J Infect Dis Volume: 64 Pages: 463-472
  • NAID: 40019126101
  • Peer Reviewed
• [Journal Article] Gene expression analysis of host innate immune responses in the central nervous system following lethal CVS-11 infection in mice. (2011)
  • Author(s): Sugiura N, Uda A, Inoue S, Kojima D, Hamamoto N, Kaku Y, Okutani A, Noguchi A, Park CH, Yamada A.
  • Journal Title: Jpn J Infect Dis. Volume: 64 Pages: 463-72
  • NAID: 40019126101
  • Peer Reviewed
• [Presentation] 野兎病菌の病原性に関与する新規因子の解析 (2013)
  • Author(s): 宇田晶彦,棚林清,シャルマニークン,堀田明豊,藤田修,森川茂
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] VNTR法を用いたヒトへの野兎病菌の伝播経路の検討 (2013)
  • Author(s): 藤田修,堀田明豊,宇田晶彦,山本美江,藤田博己,新谷史明,浅野重之,森川茂,棚林清,山田章雄
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] 日本分離Francisella tularensisの病原性および感染マウスの免疫応答 (2013)
  • Author(s): 堀田明豊,宇田晶彦,藤田修,山本美江,棚林清, Neekun Sharma,山田章雄,森川茂
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] 抗野兎病菌抗体検出用競合ELISAの開発および野生動物血清調査への応用 (2013)
  • Author(s): Neekun Sharma,堀田明豊,棚林清,山本美江,藤田修,宇田晶彦,溝口俊夫,進藤順治,朴天鎬,畑井仁,工藤上, ,山田敏文,山田章雄,森川茂
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] 日本分離Francisella tularensis の病原性および感染マウスの免疫応答 (2013)
  • Author(s): 堀田明豊, 宇田晶彦, 藤田修, 山本美江, 棚林清, Neekun Sharma, 山田章雄, 森川茂
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 岐阜
• [Presentation] 日本で分離された野兎病菌のパルスフィールドゲル電気泳動法を用いた分子疫学的解析 (2012)
  • Author(s): 藤田修,堀田明豊,宇田晶彦,棚林清,山本美江, Neekun Sharma,山田章雄
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 埼玉
• [Presentation] 日本国内で分離された野兎病菌のパルスフィールドゲル電気泳動法を用いた分子疫学的解析 (2012)
  • Author(s): 藤田修、堀田明豊、宇田晶彦、棚林清、山本美江、Neekun Sharma、山田章雄
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮
• [Presentation] 培養細胞を用いた野兎病菌の病原性比較 (2012)
  • Author(s): 堀田明豊、宇田晶彦、Sharma Neekun、藤田修、棚林清、山本美江、山田章雄
  • Organizer: 第153回日本獣医学会学術集会
  • Place of Presentation: 大宮
• [Presentation] 野兎病菌日本分離株の薬剤感受性 (2011)
  • Author(s): 堀田明豊,藤田修, Neekun Sharma,宇田晶彦,棚林清,山本美江,山田章雄
  • Organizer: 第151回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] Development of competitive ELISA for serosurvey of tularemia among various animal species. (2011)
  • Author(s): Sharma N, Hotta A, Tanabayashi K, Yamamoto Y, Fujita O, Uda A, Mizoguchi T, Shindo J, Park C H, Kudo N, Hatai H, Oyamada T, Yamada A
  • Organizer: 5th Asian Workshop on Zoo and Wildlife Medicine/ Conservation in Nepal 2011
  • Place of Presentation: ネパール