Research for the utilization of strong antibiotic activity of aurachin alkaloid

• Project/Area Number: 23790492
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YASUTAKE Yoshiaki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (20415756)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 抗生物質 / シトクロム P450 / 結晶構造 / オーラシン / シトクロムP450 / 構造生物学 / 放線菌 / アルカロイド

Research Abstract

It is important to search for novel antibiotics in order to prepare for the emergence of multi-drug resistant and/or new pathogens. In particular, antibiotics having a different molecular skeleton are needed to effectively battle such pathogens. In this s

Research Products

• [Journal Article] Cloning and heterologous expression of the aurachin RE biosynthesis gene cluster afford a novel cytochrome P450 for quinoline N-hydroxylation (2013)
  • Author(s): 北川航、尾崎太郎、西岡大樹、安武義晃、波多美弥子、西山 真、葛山智久、田村具博
  • Journal Title: ChemBioChem Volume: Vol. 14(印刷中) Issue: 9 Pages: 1085-1093
  • DOI: 10.1002/cbic.201300167
  • Peer Reviewed
• [Presentation] オーラシン類に強い抗菌活性を付与するシトクロムP450 RauAの構造機能解析 (2013)
  • Author(s): 安武義晃、北川航、西岡大樹、田村具博
  • Organizer: 物構研サイエンスフェスタ
  • Place of Presentation: エポカルつくば(茨城県)
  • Year and Date: 2013-03-14
• [Presentation] 、Structural and functional analysis of a novel cytochrome P450 RauA responsible for conferring antibiotic activity on aurachin alkaloid. A Joint Meeting of the Asian Crystallographic Association (AsCA) (2012)
  • Author(s): 安武義晃、北川航、西岡大樹、尾崎太郎、西山真、葛山智久、田村具博
  • Organizer: Society of Crystallographers in Australia and New Zealand (SCANZ) and the BRAGG Symposium
  • Place of Presentation: アデレードコンベンションセンター(オーストラリア)
  • Year and Date: 2012-12-04
• [Presentation] Intraspecies competition by antibiotics in Rhodococcuserythropolis strains (2012)
  • Author(s): 北川航、安武義晃、田村具博
  • Organizer: International Symposium on Microbial Ecology
  • Place of Presentation: ベラセンター・コペンハーゲン(デンマーク)
  • Year and Date: 2012-08-24
• [Presentation] オーラシンアルカロイドに抗菌活性を付与するシトクロムP450 (2012)
  • Author(s): 安武義晃、北川航、西岡大樹、尾崎太郎、西山真、葛山智久、田村具博
  • Organizer: 日本農芸化学会２０１２年度大会
  • Place of Presentation: 京都府京都市・京都女子大学
• [Presentation] 放線菌シトクロムP450の構造機能解析とその利用に向けた研究 (2012)
  • Author(s): 安武義晃
  • Organizer: Sapporo Symposium on advanced protein crystallography(招待講演)
  • Place of Presentation: 北海道札幌市・北海道大学
• [Presentation] Hot spot on the ferredoxin-binding site of cytochrome P450 for changing overall conformational states
  • Author(s): 安武義晃、西岡大樹、田村具博
  • Organizer: 11th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
  • Place of Presentation: トリノ
• [Presentation] Intraspecies competition by antibiotics in Rhodococcus erythropolis strains
  • Author(s): 北川航、安武義晃、田村具博
  • Organizer: International Symposium on Microbial Ecology
  • Place of Presentation: コペンハーゲン
• [Presentation] Structural and functional analysis of a novel cytochrome P450 RauA responsible for conferring antibiotic activity on aurachin alkaloid
  • Author(s): 安武義晃、北川航、西岡大樹、尾崎太郎、西山 真、葛山智久、田村具博
  • Organizer: A Joint Meeting of the Asian Crystallographic Association (AsCA), Society of Crystallographers in Australia and New Zealand (SCANZ) and the BRAGG Symposium
  • Place of Presentation: アデレード
• [Presentation] オーラシン類に強い抗菌活性を付与するシトクロムP450 RauAの構造機能解析
  • Author(s): 安武義晃、北川航、西岡大樹、田村具博
  • Organizer: 物構研サイエンスフェスタ（PFシンポジウム）
  • Place of Presentation: つくば市