| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The molecular mechanism of Ebolavirus (EBOV) entry remains to be elucidated. The long-term goal of our study is the comprehensive identification of host factors that involve in EBOV entry by siRNA screening. By use of fluorescently-labeled EBOV viral-like particles (VLPs), we succeeded to quantify the efficiency of adsorption of VLPs onto cell membrane, internalization of VLPs, and membrane fusion with endosomes. Currently we are performing siRNA screening with high-content imaging system. Our study may provide a new notion to understand molecular mechanism of EBOV entry and also contribute to the development of therapeutics against EBOV infection.
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