Identification of functional domains of influenza C virus CM2 protein involved in viral replication
| Project/Area Number |
23790494
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 病原性 / C 型インフルエンザウイルス / CM2 / C型インフルエンザ / ゲノムパッケージング |
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| Research Abstract |
Influenza C virus CM2 protein is involved in genome packaging and uncoating. To clarify the domains of CM2 participating in these functions, we introduced a series of deletion and substitution mutations in the CM2 cytoplasmic tail. We analyzed these mutants and obtained the following results.
1. C-terminal 53 amino acids of CM2 protein are not essential for its transport to the cell surface.
2. The specific amino acid sequence of cytoplasmic tail of CM2 is required for its intracellular transport.
3. The cytoplasmic tail is required for stable oligomerization (formation of a dimer and a tetramer).
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Report
(3 results)
Research Products
(13 results)
