| Project/Area Number |
23790496
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | Kyoto University |
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Principal Investigator |
HONDA Tomoyuki 京都大学, ウイルス研究所, 助教 (80402676)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細胞 / 核内封入体 / 持続感染 / リン酸化 / ウイルス / 宿主 / 封入体 / 核 / 変異 / モービリウイルス / 宿主-ウイルス間相互作用 |
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| Research Abstract |
The nucleoprotein (N) is the major component of the Morvillivirusribonucleoprotein complex (RNP) and encapsidates their non-segmented negative-stranded viral genome RNA (vRNA). The viral RNA dependent RNA polymerase complex transcribes vRNA. Morbillivirus, such as measles virus (MV), establishes a persistent infection easily and forms intranuclear inclusions. N is also the major component of this viral intranuclear inclusion. We found that fusion activity of MV was reduced during the establishment of MV persistence. Then, we demonstrated that the subcellular localization of N was regulated by its phosphorylation. Finally, we established transgenic mice expressing N by a CAG promoter (N-Tg). N-Tg formed inclusion bodies in cardiac muscle and skeletal muscle. In these muscles, N-Tg showed muscle atrophy.
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