Analysis of the newly identified receptor during VZV entry into hematopoietic cells

• Project/Area Number: 23790504
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: SUENAGA Tadahiro 大阪大学, 微生物病研究所, 助教 (20396675)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 水痘帯状疱疹ウイルス / 膜融合 / エントリー / グリコプロテインB(gB) / グリコプロテインB (gB)

Research Abstract

We have previously reported that myelin-associated glycoprotein (MAG) expressed limitedly in neural tissues is an entry receptor of varicella-zoster virus (VZV). Blood cells are also known as a gate for VZV to infect the host. The purpose of this study is to identify the VZV entry receptor on blood cells and to analyze the mechanism of VZV entry mediated by the newly identified receptor. We found that homologous molecule to MAG, VgBR2, expressed on blood cells binds to VZV glycoprotein B (gB). The association of gB not only with MAG but also with VgBR2 induces fusion between viral envelope and host cell membrane and permits VZV to enter into host blood cells (Suenaga et. al. manuscript in preparation). We also found that glycosylation on gB is involved in VZV infection mediated by MAG and VgBR2. We identified glycosylation sites and glycosylation styles on gB required for fusion mediated by MAG (Suenaga et. al. manuscript in preparation).

Research Products

• [Journal Article] 5.Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility. (2014)
  • Author(s): Jin H, Arase N, Hirayasu K, Kohyama M, Suenaga T, Saito F, Tanimura K, Matsuoka S, Ebina K, Shi K, Toyama-Sorimachi N, Yasuda S, Horita T, Hiwa R, Takasugi K, Ohmura K, Yoshikawa H, Saito T, Atsumi T, Sasazuki T, Katayama I, Lanier LL, Arase H
  • Journal Title: Proc Natl Acad Sci U S A Volume: 111 Pages: 3787-3792
  • DOI: 10.1073/pnas.1401105111
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Herpesvirus 6 Glycoproteins B (gB),gH,gL and gQ are Necessary and Sufficient for Cell-to-Cell Fusion (2013)
  • Author(s): Tanaka Y, Suenaga T, Matsumoto M, Seya T, Arase H.
  • Journal Title: J Virol Volume: 87(19) Pages: 10900-10903
  • DOI: 10.1128/jvi.01427-13
  • Peer Reviewed
• [Journal Article] Transport of misfolded endoplasmic reticulum proteins to the cell surface by MHC class II molecules (2013)
  • Author(s): Jiang, Y., Arase, N., Kohyama, M., Hirayasu, K., Suenaga, T., Jin, H., Matsumoto, M., Shida, K., L. Lanier, L., Saito, T. and Arase, H.
  • Journal Title: Int Immunol Volume: 25 Pages: 235-246
  • DOI: 10.1093/intimm/dxs155
  • Peer Reviewed
• [Presentation] Rheumatoid factor binding to IgG heavy chain presented on HLA-DR is associated with Rheumatoid Arthritis susceptibility (2013)
  • Author(s): Jin Hui,Arase Noriko,Kohayama Masako,Saito Fumiji,Hirayasu Kouyuki,Matsumoto Maki,Shida Kyoko,Suenaga Tadahiro,Saito Takashi,Katayama Ichiro,Lanier Lewis L.,Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2013-12-13
• [Presentation] 2-glycoprotein I presented on MHC class II molecules are recognized by autoantibodies in antiphospholipid syndrome (2013)
  • Author(s): Tanimura Kenji,Suenaga Tadahiro,Jin Hui,Hirayasu Kouyuki,Arase Noriko,Kohayama Masako,Ebina Yasuhiko,Yasuda Shinsuke,Horita Tetsuya,Katayama Ichiro,Atsumi Tatsuya,Yamada Hideo,Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2013-12-13
• [Presentation] PILRαnegatively regulates DSS induced experimental colitis (2013)
  • Author(s): Kishida Kazuki,Kohyama Masako,Kurashima Yosuke,Wang Jing,Hirayasu Kouyuki,Suenaga Tadahiro,Kiyono Hiroshi,Kunisawa Jun,Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ(千葉市)
  • Year and Date: 2013-12-13
• [Presentation] 水痘帯状庖疹ウイルス(VzV)の膜融合メカニズムの解析 (2013)
  • Author(s): 末永忠広、森康子、荒瀬尚
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場(神戸市)
  • Year and Date: 2013-11-10
• [Presentation] Herpesvirus 6 Glycoprotein B (gB),gH,gL and gQ are Necessary and Sufficient for Cell-to-Cell Fusion (2013)
  • Author(s): Tadahiro Suenaga,Yuki Tanaka,Misako Matsumoto,Tsukasa Seya,Hisashi Arase
  • Organizer: 38th International Herpesvirus Workshop
  • Place of Presentation: Grand Rapids,Michigan (USA)
  • Year and Date: 2013-07-21
• [Presentation] 水痘帯状庖疹ウイルス(VzV)glycoprotein H (gH)受容体の解析 (2013)
  • Author(s): 末永忠広、松本麻紀、有津史倫、森康子、荒瀬尚
  • Organizer: 第28回ヘルペスウイルス研究会
  • Place of Presentation: 淡路夢舞台国際会議場(淡路市)
  • Year and Date: 2013-05-31
• [Presentation] Human cytomegalovirus UL10 regulates immune response via inhibitory PILRα (2012)
  • Author(s): Fuminori Arisawa,Jing Wang,Tadahiro Suenaga,Hisashi Arase
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2012-12-06
• [Presentation] Transport of misfolded ER proteins to the cell surface by MHC class II molecules (2012)
  • Author(s): Yan Jiang,Hui Jin,Masako Kohyama,Noriko Arase,Kouyuki Hirayasu,Tadahiro Suenaga,Maki Matsumoto,Kyoko Shida,Lewis L. Lanier,Takashi Saito,Ichiro Katayama and Hisashi Arase
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場(兵庫)
  • Year and Date: 2012-12-06
• [Presentation] ヘルペスウイルス6型の膜融合はgB,gH,gL,gQによって引き起こされる (2012)
  • Author(s): 田中悠喜、末永忠広、松本麻紀、森康子、荒瀬尚
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2012-11-13
• [Presentation] 水痘帯状庖疹ウイルス(VzV)の感染におけるシアル酸の役割 (2012)
  • Author(s): 末永忠広、松本麻紀、有津史倫、森康子、荒瀬尚
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2012-11-13
• [Presentation] 水痘帯状庖疹ウイルス(VzV)の膜融合におけるシアル酸の役割 (2012)
  • Author(s): 末永忠広、松本麻紀、有津史倫、森康子、荒瀬尚
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ健康宿泊館プラザホール(愛知)
  • Year and Date: 2012-06-07
• [Presentation] 神経指向性ヘルペスウイルスに対する糖鎖を利用した新規感染制御法 (2011)
  • Author(s): 末永 忠広
  • Organizer: 第52回日本神経学会学術大会
  • Place of Presentation: 愛知県(名古屋国際会議場)
• [Presentation] 水痘帯状疱疹ウイルス(VZV)の膜融合機序の解析 (2011)
  • Author(s): 末永忠広、松本麻紀、森康子、荒瀬尚
  • Organizer: 第26回ヘルペスウイルス研究会
  • Place of Presentation: 大阪市（大阪アカデミア）
• [Presentation] Newly Identified Varicella-Zoster Virus(VZV) gB Receptor that Mediates Membrane Fusion and VZV Entry into Hematopoetic Cells (2011)
  • Author(s): Tadahiro Suenaga
  • Organizer: 36th International Herpesvirus Workshop
  • Place of Presentation: Gdansk（Poland）
• [Presentation] Newly Identified Varicella-Zoster Virus(VZV) Entry Receptor Expressed in Hematopoietic Cells (2011)
  • Author(s): Tadahiro Suenaga, Fuminori Arisawa, Yasuko Mori, Hisashi Arase
  • Organizer: International Union of Microbiological Societies Congresses 2011
  • Place of Presentation: Sapporo (JAPAN)
• [Presentation] A Varicella-Zoster Virus entry receptor that expresses on hematopoietic cells (2011)
  • Author(s): T.Suenaga, M.Matsumoto, Y.Mori, H.Arase
  • Organizer: 15th International Conference on Immunobiology and Prophylaxis of Human Herpesvirus Infections
  • Place of Presentation: San Servolo,Venice（Italy）
• [Presentation] Rheumatoid factor binding to IgG heavy chain presented on HLA-DR is associated with Rheumatoid Arthritis susceptibility
  • Author(s): Jin Hui, Arase Noriko, Kohayama Masako, Saito Fumiji, Hirayasu Kouyuki, Matsumoto Maki, Shida Kyoko, Suenaga Tadahiro, Saito Takashi, Katayama Ichiro, Lanier Lewis L., Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] β2-glycoprotein I presented on MHC class II molecules are recognized by autoantibodies in antiphospholipid syndrome
  • Author(s): Tanimura Kenji, Suenaga Tadahiro, Jin Hui, Hirayasu Kouyuki, Arase Noriko, Kohayama Masako, Ebina Yasuhiko, Yasuda Shinsuke, Horita Tetsuya, Katayama Ichiro, Atsumi Tatsuya, Yamada Hideo, Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] PILRα negatively regulates DSS induced experimental colitis
  • Author(s): Kishida Kazuki, Kohyama Masako, Kurashima Yosuke, Wang Jing, Hirayasu Kouyuki, Suenaga Tadahiro, Kiyono Hiroshi, Kunisawa Jun, Arase Hisashi
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉市）
• [Presentation] 水痘帯状疱疹ウイルス（VZV）の膜融合メカニズムの解析
  • Author(s): 末永忠広、森康子、荒瀬尚
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸国際会議場（神戸市）
• [Presentation] Herpesvirus 6 Glycoprotein B (gB), gH, gL and gQ are Necessary and Sufficient forCell-to-Cell Fusion
  • Author(s): Tadahiro Suenaga, Yuki Tanaka, Misako Matsumoto, Tsukasa Seya, Hisashi Arase
  • Organizer: 38th International Herpesvirus Workshop
  • Place of Presentation: Grand Rapids, Michigan, USA
• [Presentation] 水痘帯状疱疹ウイルス（VZV）glycoprotein H (gH)受容体の解析
  • Author(s): 末永忠広、松本麻紀、有澤史倫、森康子、荒瀬尚
  • Organizer: 第28回ヘルペスウイルス研究会
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市）
• [Presentation] 水痘帯状疱疹ウイルス(VZV)の膜融合におけるシアル酸の役割
  • Author(s): 末永忠広、松本麻紀、有澤 史倫、森康子、荒瀬尚
  • Organizer: 第27回ヘルペスウイルス研究会
  • Place of Presentation: あいち健康プラザ健康宿泊館プラザホール(愛知県)
• [Presentation] Glycoprotein B (gB), gH, gL, gQ1 and gQ2 of Human Herpesvirus 6 Mediate Membrane Fusion
  • Author(s): Yuki Tanaka, Tadahiro Suenaga, Maki Matsumoto, Yasuko Mori, Hisashi Arase
  • Organizer: 第11回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Presentation] Human cytomegalovirus UL10 regulates immune response via inhibitory PILRα
  • Author(s): Fuminori Arisawa, Jing Wang, Tadahiro Suenaga, Hisashi Arase
  • Organizer: 第11回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県）
• [Presentation] 水痘帯状疱疹ウイルス(VZV)の感染におけるシアル酸の役割
  • Author(s): 末永 忠広、松本 麻紀、有澤 史倫、森 康子、荒瀬 尚
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪国際会議場）（大阪府）
• [Presentation] ヘルペスウイルス6型の膜融合はgB, gH, gL, gQによって引き起こされる
  • Author(s): 田中 悠喜、末永 忠広、松本 麻紀、森 康子、荒瀬 尚
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪国際会議場）（大阪府）
• [Presentation] Transport of misfolded ER proteins to the cell surface by MHC class II molecules
  • Author(s): Jiang, Y., Arase, N., Kohyama, M., Hirayasu, K., Suenaga, T., Jin, H., Matsumoto, M., Shida, K., L. Lanier, L., Saito, T. and Arase, H.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] Human cytomegalovirus UL10 regulates immune response via inhibitory PILRα
  • Author(s): Fuminori Arisawa, Jing Wang, Tadahiro Suenaga, Hisashi Arase
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Remarks]
  • URL: http://immchem.biken.osaka-u.ac.jp/
• [Remarks] 免疫化学分野ホームページ
  • URL: http://immchem.biken.osaka-u.ac.jp/
• [Remarks] 大阪大学微生物病研究所免疫化学分野
  • URL: http://immchem.biken.osaka-u.ac.jp/
• [Remarks]
  • URL: http://immchem.biken.osaka-u.ac.jp/