Molecular mechanism of anti-HIV-1 activity mediated by Rac2

• Project/Area Number: 23790510
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Kinki University
• Principal Investigator: HAKATA Yoshiyuki 近畿大学, 医学部, 助教 (30344500)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ウイルス / HIV-1 / rac2 / 感染抵抗性因子 / 感染制御 / Rac2 / 感染抵抗性遺伝子

Research Abstract

We identified a new function of human Rac2. Rac2 negatively or positively regulates several cytokines production at transcriptional level. The transcriptional regulation by Rac2 involves specific molecules acting in cellular signal transduction pathway. The regulation of inflammatory cytokines would be the one of mechanisms by which Rac2 mediates the anti-HIV-1 activity. We found that Rac2 associates with viral proteins which can control autophagy. These interactions implied that Rac2 suppresses HIV-1 replication thorough modification of cellular autophagy activity controlled by viral proteins.

Research Products

• [Journal Article] Two genetic determinants acquired late in Mus evolution regulate the inclusion of exon 5, which alters mouse APOBEC3 translation efficiency (2012)
  • Author(s): Li, J., Y. Hakata, E. Takeda, Q. Liu, Y. Iwatani, C. A. Kozak, M. Miyazawa
  • Journal Title: PLoS Pathogens Volume: 8 Issue: 1 Pages: e1002478-e1002478
  • DOI: 10.1371/journal.ppat.1002478
  • NAID: 120006985441
  • Peer Reviewed
• [Presentation] Vpr依存的G2期停止におけるVprと宿主因子との分子間相互作用 (2012)
  • Author(s): 博多 義之
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場
• [Presentation] Functional consequences of mouse APOBEC3 gene polymorphisms and multiple genetic factors that influence the production of virus-neutralizing antibodies in Friend virus-infected mice (2011)
  • Author(s): Miyazawa, M., S. Tsuji-Kawahara, Y. Hakata, J. Li, E. Takeda, and C. Ishihara
  • Organizer: The 23rd Workshop on Retroviral Pathogenesis
  • Place of Presentation: Montpellier, France
• [Remarks]
  • URL: http://www.med.kindai.ac.jp/immuno/
• [Remarks] 近畿大学医学部免疫学教室
  • URL: http://www.med.kindai.ac.jp/immuno/